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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Will a bacterial contamination always spread?
#18871917 - 09/21/13 12:24 PM (10 years, 4 months ago) |
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I noced up a few jars about ~6 days ago with some questionable syringes (made from outdoor prints, so who knows how many contaminant spores are in them). After about 24 hours there was a wet spot on the bottom of each point where I inoculated it. It could just be excess water from the inoculate solution or it could be bacterial contamination, it's hard to tell at this point and I don't want to shake them until the mycilium has a good foothold. So what I'm wondering is if it is bacterial will it typically spread or might it look the same up until a shake?
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Will a bacterial contamination always spread? [Re: krypto2000]
#18877265 - 09/22/13 06:27 PM (10 years, 4 months ago) |
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Don't shake if you do have bacteria and yes it will spread. More slowly than the mycelium.
Does it smell?
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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krypto2000
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Re: Will a bacterial contamination always spread? [Re: Ganzig]
#18885472 - 09/24/13 03:40 PM (10 years, 4 months ago) |
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Well I ended up tossing them as they showed signs of other contaminants. I do now have a jar with a single grain at the bottom which I suspect is bacterial though. It's otherwise fully colonized and the mycilium may just be having trouble colonizing that grain in which case it's metabolites. I'm going to keep an eye on it and if the myc overtakes it I'll spawn, otherwise I may try to spawn it anyhow and see how it does, it's only a single grain.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Will a bacterial contamination always spread? [Re: krypto2000]
#18886107 - 09/24/13 06:13 PM (10 years, 4 months ago) |
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What is your set up?
Flow hood? Still air box?
Do you work with agar?
If I were you I would make some 1/2 pint jars with agar and inoculate the agar with one of the grains from the top of the jar that is colonized.
In a still air box. No need to stir up those bacteria.
Get a clean culture and start over.
And PC your jars longer.
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krypto2000
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Re: Will a bacterial contamination always spread? [Re: Ganzig]
#18886184 - 09/24/13 06:39 PM (10 years, 4 months ago) |
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Well I could just not use that one jar, I have extras, but then I'm left with ~6 healthy jars and 1 small possible bacterial contam. Do you think it'd be better to just not use it all together? I figured it's so small what can it hurt?
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Will a bacterial contamination always spread? [Re: krypto2000]
#18886214 - 09/24/13 06:48 PM (10 years, 4 months ago) |
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It can hurt a lot. Getting rid of bacteria and their endospores can be a pain in the arse. I can attest to that. It took me completely bleaching everything in my lab to stop the spread.
It was a bummer.
I say get rid of it if you have healthy jars. Don't clean bacteria laden jars and then work on other myc work on the same day.
New clean clothes after a shower. I wear a full tyvek suit that I sterilize before lab work. I sterilize it in my PC.
It may seem like over kill but my lab had such a bad bacteria problem that I am forever burned by it and afraid of it.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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krypto2000
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Re: Will a bacterial contamination always spread? [Re: Ganzig]
#18886863 - 09/24/13 09:39 PM (10 years, 4 months ago) |
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Well now I can't even find the jar . I'm assuming the myc has already colonized that grain and it was just metabolites. There is one jar with a good bit of metabolites in the bottom (compared to other ones), but I can't find the grain spot in it so not sure if that's it or not.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Will a bacterial contamination always spread? [Re: krypto2000]
#18888339 - 09/25/13 08:53 AM (10 years, 4 months ago) |
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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krypto2000
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Registered: 12/05/06
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Last seen: 4 years, 3 months
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Re: Will a bacterial contamination always spread? [Re: Ganzig]
#18888497 - 09/25/13 09:39 AM (10 years, 4 months ago) |
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It actually was the jar with the metabolites in the bottom upon inspecting them again last night, not sure how I missed it. Still going to wait and see what happens with it, myc can eat some bacteria anyway afaik, so maybe it's just in the process of eating it. The mycilium is still growing either way, just rather slowly. At this rate I'd expect it to be colonized within another couple of days, if not I'll probably spawn it outside.
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Kizzle
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Registered: 08/30/11
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Re: Will a bacterial contamination always spread? [Re: Ganzig]
#18889224 - 09/25/13 01:07 PM (10 years, 4 months ago) |
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Quote:
Ganzig said: It can hurt a lot. Getting rid of bacteria and their endospores can be a pain in the arse. I can attest to that. It took me completely bleaching everything in my lab to stop the spread.
It was a bummer.
I say get rid of it if you have healthy jars. Don't clean bacteria laden jars and then work on other myc work on the same day.
New clean clothes after a shower. I wear a full tyvek suit that I sterilize before lab work. I sterilize it in my PC.
It may seem like over kill but my lab had such a bad bacteria problem that I am forever burned by it and afraid of it.
Keep in mind simply being in a room adds 37 million bacteria to the air in it an hour. You're by far the largest source of bacteria in your lab even larger than contaminated jars since your constantly breathing it into the air.
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krypto2000
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Re: Will a bacterial contamination always spread? [Re: Kizzle]
#18890081 - 09/25/13 04:21 PM (10 years, 4 months ago) |
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Are you saying even if I open a contaminated jar it's roughly the same or not significantly worse than me simply being in the room moving around?
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Will a bacterial contamination always spread? [Re: krypto2000]
#18890712 - 09/25/13 06:47 PM (10 years, 4 months ago) |
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Not exactly. But say, opening a bacteria contaminated jar and dumping it in the toilet isn't going to have a much of different effect than what you normally dump in the toilet.
While mold spores are long lived and can have a lasting affect on concentrations in an environment, bacteria is much is easier to deal with once the source of the bacteria has been removed. In most cases when your substrates get contaminated with bacteria it's the cultivator that is the source usually in combination with flaws in sterile technique.
And in fact the chance of one of the few endospore forming endospore bacteria landing on surface, forming an endospore before desiccation sets in it's disinfected, and most importantly then becoming airborne again to infect a culture is very small. Most bacteria in our air is because we're spewing it out, it doesn't become airborne otherwise that easily.
Edited by Kizzle (09/25/13 06:54 PM)
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