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OfflineR4V4N4
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Spawn bag 0% growth in 22 days
    #18870408 - 09/21/13 12:44 AM (10 years, 4 months ago)

I got Freestone Texas
was very sterile

injected spawn bag with 2.5 cc

kept temp 68-85 (usually 75-82)
poor lighting
_____________________________________

I did not refrigerate my syringe ond put a cap on
black particles are floating in there

should I inject the Orissa now that it looks like I may have had a dud? should I just start injecting everything growbags too now that the spawn may be a failure?


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Edited by R4V4N4 (09/21/13 01:42 AM)


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OfflineMush4Brains
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Re: Spawn bag 0% growth in 22 days [Re: R4V4N4]
    #18870430 - 09/21/13 12:57 AM (10 years, 4 months ago)

It isn't recommended to try to re-inoculate the same bag/jar more than once.  After three weeks it is likely your grains are drying out.  If you're going to do nothing but throw the bag away anyways, I guess it can't hurt, but it is usually just a waste of spores.  Hopefully you have more grain/bags to try again.


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Offlinefatchillin
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Re: Spawn bag 0% growth in 22 days [Re: Mush4Brains]
    #18870447 - 09/21/13 01:09 AM (10 years, 4 months ago)

take out the vendor name in your post  :rules:  But im agreeing with the above post ^

it could be a variety of issues, im not certain which pertains to your situation.


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Offlinekinkaku
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Re: Spawn bag 0% growth in 22 days [Re: Mush4Brains]
    #18870450 - 09/21/13 01:10 AM (10 years, 4 months ago)

Quote:

Mush4Brains said:
It isn't recommended to try to re-inoculate the same bag/jar more than once.  After three weeks it is likely your grains are drying out.  If you're going to do nothing but throw the bag away anyways, I guess it can't hurt, but it is usually just a waste of spores.  Hopefully you have more grain/bags to try again.



what he said:thumbup:


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Offlinehidyn
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Re: Spawn bag 0% growth in 22 days [Re: kinkaku]
    #18870468 - 09/21/13 01:15 AM (10 years, 4 months ago)

Aww man, that's such a shame. I feel your pain. :frown:


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OfflineR4V4N4
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Re: Spawn bag 0% growth in 22 days [Re: hidyn]
    #18870547 - 09/21/13 01:50 AM (10 years, 4 months ago)

Ok it may have been 13-22 days but I just looked at it now and its 1% colonized

saved by hair on teeth?


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OfflineMush4Brains
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Re: Spawn bag 0% growth in 22 days [Re: R4V4N4]
    #18870554 - 09/21/13 01:52 AM (10 years, 4 months ago)

If you insist :shrug:.  Although Psilly will see this and say no (:lol:), colonization can take 3+ weeks to start.


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OfflineR4V4N4
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Re: Spawn bag 0% growth in 22 days [Re: Mush4Brains]
    #18870627 - 09/21/13 02:24 AM (10 years, 4 months ago)



amidoinitrite?


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InvisibleKatuXiK
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Re: Spawn bag 0% growth in 22 days [Re: R4V4N4]
    #18870721 - 09/21/13 03:15 AM (10 years, 4 months ago)

That looks a lot different than what I have in my jars.. I'm a first timer right now (wish me luck) so I don't have much to compare it to, but it'd be awesome if your bag recovers!


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OfflineGoldenArrow
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Re: Spawn bag 0% growth in 22 days [Re: KatuXiK]
    #18870758 - 09/21/13 03:46 AM (10 years, 4 months ago)

that bag's too wet, that's bacteria IMO


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OfflineR4V4N4
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Re: Spawn bag 0% growth in 22 days [Re: KatuXiK]
    #18870826 - 09/21/13 04:38 AM (10 years, 4 months ago)

Thank you KatuXiK

Welcome to Teh Shroomery!
Your first post outside  your thread on my thread!
I hope that this symbolic gesture will lead to us being BFFs Best Fungal Friends

I do not know enough about the appearance of early colonization but I think I have Mycelium or possible bacteria

Also the picture makes the bag somewhat more wet than it is IMO


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Edited by R4V4N4 (09/21/13 06:34 AM)


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InvisibleKatuXiK
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Re: Spawn bag 0% growth in 22 days [Re: R4V4N4]
    #18871703 - 09/21/13 11:30 AM (10 years, 4 months ago)

Maybe BFF's we shall be, time will tell!

And when I noticed the very first mycelium in my jars, it was more of a small foggy spot and it was not immediately obvious whether it was wet BRF, or something actually growing. I'll see if I can get a picture of one that might be starting up now, although 3 of my jars have been dormant for almost 13 days now, while the other 5 have been growing, so I'm really hoping their little foggy spots are mycelium.


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OfflineRauhfasertapete
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Re: Spawn bag 0% growth in 22 days [Re: KatuXiK]
    #18871754 - 09/21/13 11:45 AM (10 years, 4 months ago)

Even if it was well sterilised and you had worked cleanly, your grain won´t be sterile anymore after 3 weeks. one single bacterium is enough, the bubbly spots even indicate something like yeast. Lots of litte creatures producing their own toxins to keep all other organisms (like mushrooms) away. This grain is not edible anyomre for any higher organism, even if you sterilise again and use a healthy LC, a mushroom will have difficulties to cope with the bacterial toxins.

BTW: moisture makes it easier for bacteria, but if they multiply in there anyway, they may also produce thos moist spots by themselves


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OfflineR4V4N4
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Re: Spawn bag 0% growth in 22 days [Re: Rauhfasertapete]
    #18873087 - 09/21/13 05:12 PM (10 years, 4 months ago)

when i press air through the filter patch it smells like cooked rye berries. is that a good sign?


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OfflineMush4Brains
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Re: Spawn bag 0% growth in 22 days [Re: R4V4N4]
    #18873140 - 09/21/13 05:28 PM (10 years, 4 months ago)

It's a sign that you don't have a contamination that produces an odor.


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InvisibleSpitballJedi
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Re: Spawn bag 0% growth in 22 days [Re: Mush4Brains]
    #18873224 - 09/21/13 06:00 PM (10 years, 4 months ago)

Your grains are too wet and I don't see anything that looks like mycelium.

On a side note, I tried bags a few times and did poorly. The moisture was hard to get right. I went back to jars...much easier.


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OfflineInsomnia411
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Re: Spawn bag 0% growth in 22 days [Re: SpitballJedi]
    #18874326 - 09/21/13 11:45 PM (10 years, 4 months ago)

i agree with Spitballjedi.  I have tried bags several times because I thought it would be lower maintenance, but they were always very quick to contam or were just plain duds.  Not really sure why.  Btw, I tried it with both rye and WBS, same result.


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