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OfflineSockadin
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First Clone attempt with Agar (advise)
    #18868821 - 09/20/13 05:45 PM (10 years, 4 months ago)

So I just attempted to clone my big fat KSSS on Potatoe Dex and Agar.

First I boiled a pot with 10g Agar Agar, 10 G Potatoe Dextrose.

Then I put in in a jar and PC'd for 45 mins and let cool for 45 mins.

Then I got my SAB and loaded and cleaned it with all my crap.

  • Agar Jar
  • 4 plastic petri dish's
  • Clone material (stem of fatty)


I poured the dish, and added stem material, covered and taped.


Am I missing anything?

I suck at agar, and only have crappy plastic petri's.

  Any thoughts?


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OfflineMush4Brains
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Registered: 07/31/13
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Re: First Clone attempt with Agar (advise) [Re: Sockadin]
    #18868843 - 09/20/13 05:49 PM (10 years, 4 months ago)

You let the agar solidify first, right?


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OfflineSockadin
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Re: First Clone attempt with Agar (advise) [Re: Mush4Brains]
    #18868850 - 09/20/13 05:51 PM (10 years, 4 months ago)

Yeah cause it does an amazing job when I poor it if has solidified.


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OfflineMush4Brains
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Re: First Clone attempt with Agar (advise) [Re: Sockadin]
    #18868854 - 09/20/13 05:52 PM (10 years, 4 months ago)

No need to be a smartass, I meant before you put the tissue on the agar.


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InvisiblebodhisattaMDiscordReddit
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Re: First Clone attempt with Agar (advise) [Re: Sockadin]
    #18868861 - 09/20/13 05:54 PM (10 years, 4 months ago)

He means did you let it cool down and solidify in the dish before putting your tissue sample in.


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OfflineSockadin
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Re: First Clone attempt with Agar (advise) [Re: bodhisatta]
    #18868887 - 09/20/13 06:01 PM (10 years, 4 months ago)

Oh yeah, nope.  This is why I was asking people smarter than me.

I don't know anything about agar, so I should have let the agar cool before adding in my stem? Let me put it this way, it was not hot to the touch, probably about 80 degrees. I was worried that if I let it cool to much it would be too cool to drop in my stem material.

I could be wrong, and will probably get flamed for this post, but hey, you don't know what you don't know and I am in the process of learning.


Thanks for everyones help and advise.


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Invisiblecynical bastad
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Registered: 08/17/13
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Re: First Clone attempt with Agar (advise) [Re: Sockadin]
    #18868923 - 09/20/13 06:13 PM (10 years, 4 months ago)

Maybe read some more?

:shrug:


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InvisibleSpitballJedi
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Re: First Clone attempt with Agar (advise) [Re: Sockadin]
    #18868987 - 09/20/13 06:31 PM (10 years, 4 months ago)

You'll be fine. You'll need to do a 1 or 2 transfers anyway, to make sure it's not contaminated.

The benefit of letting it cool to solid is so you have a plane for it to grow out on. Unless you buried it or pushed it to the bottom, that's what will likely happen anyway. This makes it easier to spot contams (and sectors when you are isolating)


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OfflineSockadin
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Re: First Clone attempt with Agar (advise) [Re: SpitballJedi]
    #18869466 - 09/20/13 08:30 PM (10 years, 4 months ago)

Thanks for the advise, I see know that it makes more sense to let it solidify so that it does not sink. I however did not do this, so we will have to wait and see what happens.

Thanks Spitball.


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