Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 2 years, 8 months
BRF jars doubt
    #18868470 - 09/20/13 04:20 PM (10 years, 4 months ago)

Hey second time grow planning on going from some BRF jars into a few coir monotub probably using Damion5050's Coir Tek but i'll worry about that when the time comes.
  Just wondering, the other day i had to fill up three jars with the standard BRF mix (2:1:1) and then thought if it might be a problem that i was going to boil/sterilize a few days later, and then inoculate, maybe contams will show up in the meantime? I'm planning on doing about 12 jars total and they're just over half a pint by the looks of it. Any advice is welcome.:mushroom2: :peace:


--------------------
:awecid:


Extras: Filter Print Post Top
OfflineStromriderM
This must be the place
Male User Gallery


Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say... Flag
Last seen: 1 hour, 39 minutes
Trusted Cultivator
Re: BRF jars doubt [Re: Madoxx]
    #18868594 - 09/20/13 04:46 PM (10 years, 4 months ago)

I say better safe than sorry. They would probably be ok but I think it is always better to steam right after mixing and filling. It would be incredibly cheap and easy to just re do them so why not :shrug:


Extras: Filter Print Post Top
OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 2 years, 8 months
Re: BRF jars doubt [Re: Stromrider]
    #18870729 - 09/21/13 03:20 AM (10 years, 4 months ago)

Yeah i guess, thanks for the quick response  :irishtoast:


--------------------
:awecid:


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
Trusted Cultivator
OG Cultivator
Re: BRF jars doubt [Re: Madoxx]
    #18871738 - 09/21/13 11:39 AM (10 years, 4 months ago)

Quote:

Madoxx said:

  Just wondering, the other day i had to fill up three jars with the standard BRF mix (2:1:1) and then thought if it might be a problem that i was going to boil/sterilize a few days later,




Don't do this.  Sterilization is rarely perfect, so don't increase the contaminant load unnecessarily.  Fill the jars and then load and sterilize.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 2 years, 8 months
Re: BRF jars doubt [Re: RogerRabbit]
    #18879260 - 09/23/13 05:07 AM (10 years, 4 months ago)

Great going to do the whole process tonight, from some great Z-strain print i got sent to inoculation. Another doubt, watched RR's videos for PF-tek but i'm lacking 20 extra jar lids to put at the bottom of the pan, sensible alternatives? Maybe a layer of rocks, tin foil, repeat?


--------------------
:awecid:


Extras: Filter Print Post Top
OfflineStromriderM
This must be the place
Male User Gallery


Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say... Flag
Last seen: 1 hour, 39 minutes
Trusted Cultivator
Re: BRF jars doubt [Re: Madoxx]
    #18879705 - 09/23/13 09:13 AM (10 years, 4 months ago)

Yeah rocks or gravel will work. Foil balls will work as well.


Extras: Filter Print Post Top
OfflinePrinceShroom
Experienced Mofo
Male User Gallery


Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 9 months
Re: BRF jars doubt [Re: Stromrider]
    #18879769 - 09/23/13 09:28 AM (10 years, 4 months ago)

Plate or a tea towel works too


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


Extras: Filter Print Post Top
OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 2 years, 8 months
Re: BRF jars doubt [Re: Stromrider]
    #18880423 - 09/23/13 12:37 PM (10 years, 4 months ago)

Perfect will report back:mushroom2::peace:


--------------------
:awecid:


Extras: Filter Print Post Top
OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 2 years, 8 months
Re: BRF jars doubt [Re: Stromrider]
    #18976485 - 10/14/13 12:57 PM (10 years, 3 months ago)

Okay se here's the update! Ended up inoculating 8 BRF jars due to lack of more spores (i had 2 good and another shitty spore print, shouldn't i have been able to make more than 5 syringes).
    Out of those 8 5 got contams, one didn't respond and got two successfull jars so i have to look into my sterile technique. Anyway with those two i crumbled them and spawned to Coir/Verm mix using Damion's Elementary Tek.
    I Just put the trays into fruiting conditions in two boxed with a gauze to act as an air filter. Looking like a pretty healthy mycelium which colonized quickly. If i get some good quality pictures i much do a follow-up post with timing and some pinning porn. :peace::peace:





--------------------
:awecid:


Extras: Filter Print Post Top
OfflineSpinScratch
Distrusted Cultivator
Male

Registered: 07/26/13
Posts: 826
Last seen: 29 days, 21 hours
Re: BRF jars doubt [Re: Madoxx]
    #18976587 - 10/14/13 01:20 PM (10 years, 3 months ago)

8 jars inoculated from 5 syringes?? were they 10cc? you could have done at least 25 jars with that. I usually put 1/2 an ML in each hole. The less you put in, the less contamination you will have. Along with proper sterilization and aseptic technique.


Extras: Filter Print Post Top
OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 2 years, 8 months
Re: BRF jars doubt [Re: SpinScratch]
    #18980610 - 10/15/13 09:39 AM (10 years, 3 months ago)

Yeah 8 jars did feel like waaay too little but i knew no better plus i had no internet to check. 0.5mL and you get successful inoculations all the time? I put around 2.5ml in each hole hahaha i still have a 'few' details to polish.
Could you give me and idea of the consistency of the syringe? like does it look completely clear or can you see spores? I had one Ecuador syringe which looked almost clear and would not have been comfortable adding even 1ml


--------------------
:awecid:


Extras: Filter Print Post Top
OfflineSpinScratch
Distrusted Cultivator
Male

Registered: 07/26/13
Posts: 826
Last seen: 29 days, 21 hours
Re: BRF jars doubt [Re: Madoxx]
    #19150650 - 11/17/13 04:47 PM (10 years, 2 months ago)

they say you only need 1 ML per jar. So thats 0.25 ML per hole. Just make sure you shake up the syringe really hard. theres millions of spores in there. You will have spores in every squirt.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: BRF jars doubt [Re: SpinScratch]
    #19150780 - 11/17/13 05:11 PM (10 years, 2 months ago)

Spore prints are not sterile so to cut down on the number of contams that pop up we use as little solution as possible. You could probably get away with a few drops per hole. Next time you will do better, you got the Shroomery on yer side now :awesomenod:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* BRF jar to jar innoculation Ether_Bunny 2,129 1 02/06/02 11:17 AM
by MickyFinn
* Condensation in BRF jars ? Action_Man 2,845 4 02/20/02 12:01 AM
by moxjet20
* birdseed vs. verm/brf jars..whcih is easier4aNEWB? pimp420 2,826 12 06/06/02 02:44 PM
by pimp420
* brf jars Anonymous 1,573 9 10/12/02 07:04 PM
by Anonymous
* Post deleted by Moe Howard Anonymous 619 1 10/16/02 09:23 PM
by Skikid16
* Psilocybe Cyanescens on BRF! I DID IT! Zenmaster6 1,011 9 04/11/18 08:03 PM
by Shrooms4life
* Brf + Wormcastings substrate SlamDog_C 1,837 5 04/13/02 05:14 AM
by SlamDog_C
* rye berry jars no white stuff? ehud 4,872 14 09/30/09 01:54 PM
by khines72

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
867 topic views. 37 members, 217 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.008 seconds on 14 queries.