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Jw6457
Stranger

Registered: 12/17/11
Posts: 434
Last seen: 9 years, 8 months
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Slow bulk colonization, multiple tubs
#18867404 - 09/20/13 12:11 PM (10 years, 4 months ago) |
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Hey guys, just wondering if using multipspore to many masters to g2g transfers. Can using the g2g jars as spawn for tubs still be slow to colonize tubs considering the mixing of many genetics?
I ask this because a lot of my tubs are taking long periods of time to colonize some as far as 18 days and still not done. Im wondering if i should open up the longest one which will be colonizin for 3 weeks that only has a few spots not colonized and sniff to see if its good.
Can anyone explain if using ms can take a lot lknger to colonize bulk sub?
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Jw6457
Stranger

Registered: 12/17/11
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Last seen: 9 years, 8 months
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Re: Slow bulk colonization, multiple tubs [Re: Jw6457]
#18874031 - 09/21/13 09:56 PM (10 years, 4 months ago) |
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Anybody?
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Slow bulk colonization, multiple tubs [Re: Jw6457]
#18874051 - 09/21/13 10:05 PM (10 years, 4 months ago) |
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could be genetics or even low spawn to bulk ratios. What are your ratios?
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Jw6457
Stranger

Registered: 12/17/11
Posts: 434
Last seen: 9 years, 8 months
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Re: Slow bulk colonization, multiple tubs [Re: mushmagic]
#18877702 - 09/22/13 08:04 PM (10 years, 4 months ago) |
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Ratio is a lil more then 1:1 ratio. 6 qts of spawn to 7 qts of bulk sub so i have no friggin idea why its taking nearly this long. It should colonize in no time ime. So thats why i wonder if its moisture or ge or obviously contams
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Slow bulk colonization, multiple tubs [Re: Jw6457]
#18877824 - 09/22/13 08:34 PM (10 years, 4 months ago) |
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Bacterial contam. Your lid provides plenty of GE for colonizing bulk.
Take some pictures, please. It will help ID your issue.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Slow bulk colonization, multiple tubs [Re: Jw6457]
#18877843 - 09/22/13 08:39 PM (10 years, 4 months ago) |
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could be a few things.
What's the temperature in the room like? What is your exact substrate recipe?
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Jw6457
Stranger

Registered: 12/17/11
Posts: 434
Last seen: 9 years, 8 months
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Re: Slow bulk colonization, multiple tubs [Re: SpitballJedi]
#18880996 - 09/23/13 03:11 PM (10 years, 4 months ago) |
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I think it might be moisture problem since i dont use liners and the sides of the tub are where the spots that are still slow to colonize are. I raised the temps a little bit to around 75 but mainly 68-72 its been. I also think i might have sealed the tubs up to much so its been hard for ge to occur. Seems like some are starting to come around since i dumped the excess water buildup and took some tape off the lids. I dont have latches so figured taping it down would help that.
Or like frank said i have a shit ton of bacterial contams. Thanks guys i really appreciate it! I guess ill just have to wait and see what happens
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HybridprX
Biodegrader of coir



Registered: 01/29/08 
Posts: 2,588
Loc: Canada
Last seen: 6 years, 4 months
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Re: Slow bulk colonization, multiple tubs [Re: Jw6457]
#18881070 - 09/23/13 03:33 PM (10 years, 4 months ago) |
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Sounds to me like a FAE issue more so then humidity. The edges of the tub are the most humid. I get myc crawling the sides all the time.
Or it could have dried out.
If it does not smell sweet or foul after 18 days and strongly colonized, I'm going to put my money on a water content issue.
Sometimes people will get a stubborn patch that refuses to colonize with elevated Co2 present.... If you vent it for a few hours and then let it go for another day the patch will normally colonize before pinning occurs.
I've been waiting for my tubs to colonize, dunking, and then covering with wax paper until primorda formations form. Works like a charm, some cubensis suck at fruiting via MS.
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Jw6457
Stranger

Registered: 12/17/11
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Last seen: 9 years, 8 months
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Re: Slow bulk colonization, multiple tubs [Re: HybridprX]
#18881431 - 09/23/13 05:18 PM (10 years, 4 months ago) |
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Ya i need to just stop being a bitch an work with agar. I got g2g down pretty well so time to move on.
Is there any way my issue could be due to low amounts of verm used per brick of coir? I only eye it out and probably i add maybe 1 quart worth of verm to a brick of coir. I read that not enough verm equals more compacted bulk sub making it harder for myc to colonize?
Could this be possible suspect?
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HybridprX
Biodegrader of coir



Registered: 01/29/08 
Posts: 2,588
Loc: Canada
Last seen: 6 years, 4 months
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Re: Slow bulk colonization, multiple tubs [Re: Jw6457]
#18883691 - 09/24/13 07:00 AM (10 years, 4 months ago) |
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Could be op, compacted subs won't colonize fully.
I've had subs get compacted, fruit get to 2nd and go moudly....usually if it moulds up before 3rd the bulk was not fully colonized from everytime Ive got contams before/during the 3rd.
Try fruiting op, the longest a sub should take is 21 days.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Slow bulk colonization, multiple tubs [Re: HybridprX]
#18884698 - 09/24/13 12:19 PM (10 years, 4 months ago) |
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Quote:
HybridprX said: Could be op, compacted subs won't colonize fully.
I've had subs get compacted, fruit get to 2nd and go moudly....
That sounds like a bacterial contam.
Looks uncolonized, growth stalls, pins form. Then mold comes on the second flush.
That's a textbook bacteria issue, and it comes from either your spawn or poor pasteurization.
Fruit it OP, you may get some food out of the deal.
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Jw6457
Stranger

Registered: 12/17/11
Posts: 434
Last seen: 9 years, 8 months
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Re: Slow bulk colonization, multiple tubs [Re: FrankHorrigan]
#18886138 - 09/24/13 06:23 PM (10 years, 4 months ago) |
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Will do guys thanks, its weird i could be wrong but some parts seem to be finishing colonizing since i stuffed polyfill in the holes. Can anybody tell how to deal with condensation running down the walls of the tub and wetting the bulk sub making it hard to colonize it?
-------------------- WHAT UP GUYS< JUST HERE TRYING TO BECOME A SCIENTIST AND GROW SOME MOOSHIES
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