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OfflineDDAWGG
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Registered: 09/20/13
Posts: 5
Last seen: 10 years, 5 months
dried sclerotia as inocculant
    #18866577 - 09/20/13 08:20 AM (10 years, 6 months ago)

Hey!

I'm not very well versed with mushroom cultivation, but I've recently discovered that dry sclerotia can succesfully be used as spawn. I felt that I should contribute this as I didn't find anything on these forums to suggest that this was possible.

My technique was simple, requiring the following:
- ~30 candles
- sharp blade
- 70% rubbing alcohol
- sterilized grain jars (rye grain tek)
- tinfoil
- dried sclerotia
- cotton balls
- lighter

step 1: clean surface of table with rubbing alcohol (fucked up my varnish, FYI :S)
step 2: make circle of candles around working area on table (heat rises and moves outwards, preventing airborne particles from falling inside the work area; creates a sterile environment). I left a ~20cm "opening" in the circle of candles so that I could work inside of it.
step 3: light candles
step 4: lay down tinfoil, with inside (clean) side facing up. Place jars in back of circle (away frmo entrance).
step 5: clean tinfoil with cotton ball soaked in alcohol
step 6: clean knife with cotton ball soaked in alcohol
step 7: place dried sclerotia at entrance to your working area (keep in mind the outside of sclerotia is coated in contaminats)
step 8: thoroughly clean hands and forearms with rubbing alcohol.
step 9: pick up lighter and flame-sterilized blade.
step 10:cut 1/6 sides of sclerotia off (the goal is to cut a cube, such that no outside edges remain on the sclerotia).
step 11: move sclerotia forward ~1 inch forward into work area (1" farther into the circle)
step 12:unless you have completed your cube of sterile sclerotia, go back to step and, and make the next slice of your sclerotia cube.
step 13: sterilize hands with alcohol
step 14: remove ring from jar
step 15: sterilize hands with alcohol
step 16: pick up sterile sclerotia cube with one of your sterile hand.
step 17: open lid with other sterile hand ~1cm and insert sclerotia cube with other hand.work quickly to minimize time lid is open (half second)
step 18: close jar with ring, store at room temp.

It's important to always keep in mind that the outside edges of  sclerotia are dirty, both with respect to moving it forward onto sterile tinfoil after each slice, and also with respect to handling it between slices.es.

After ~1 day there was mycellium growing from the sclerotia. ~after 2 days there was thick growth coming from the scleorita, spreading to the grains. I used wheat. It's been a week and no signs of contamination so far.

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InvisibleFrankHorrigan
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Registered: 01/04/11
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Re: dried sclerotia as inocculant [Re: DDAWGG] * 1
    #18866617 - 09/20/13 08:30 AM (10 years, 6 months ago)

:smbfacepalm:

Alcohol does not sterilize, it sanitizes.

You should wait to post crappy technique until you learn that it is crappy.

This is misleading to new cultivators and you don't even know if it worked. A week is not long enough to see contams 95% of the time.

Best to not share "experimental" techniques until you get a harvest from it.

Welcome to the shroomery!


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Edited by FrankHorrigan (09/20/13 08:42 AM)

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OfflineGoldenArrow
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Re: dried sclerotia as inocculant [Re: FrankHorrigan]
    #18866730 - 09/20/13 08:56 AM (10 years, 6 months ago)

Wow you didn't even go to agar?

Let's hope that's not trich growing in the jars...what's your backup plan?

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InvisiblebodhisattaMDiscordReddit
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Posts: 61,891
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Re: dried sclerotia as inocculant [Re: FrankHorrigan]
    #18866741 - 09/20/13 09:00 AM (10 years, 6 months ago)

I like the circle of candles though. It's like a contamination séance.

When you cut your "cube" out of the sclerotia the blade drags contamination through the whole slice because it touches the outside first.


Edited by Trusted cuItivator (09/20/13 09:03 AM)

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Invisiblemaddchef
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Re: dried sclerotia as inocculant [Re: bodhisatta]
    #18866797 - 09/20/13 09:19 AM (10 years, 6 months ago)

Ya know the candle idea isn't half bad. Well except for that big wide open part in the middle, ya know, your workspace


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:

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InvisibleFrankHorrigan
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Posts: 10,573
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Re: dried sclerotia as inocculant [Re: maddchef]
    #18866804 - 09/20/13 09:20 AM (10 years, 6 months ago)

It's about as fantastic as the "oven tek" and we all know how well that works :rolleyes:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflineDDAWGG
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Registered: 09/20/13
Posts: 5
Last seen: 10 years, 5 months
Re: dried sclerotia as inocculant [Re: FrankHorrigan] * 1
    #18866805 - 09/20/13 09:20 AM (10 years, 6 months ago)

Quote:

GoldenArrow said:
Wow you didn't even go to agar?

Let's hope that's not trich growing in the jars...what's your backup plan?




Don't have one...I have other jars that were inocculated with LC or spores...this was an experiment
Quote:

FrankHorrigan said:
:smbfacepalm:

Alcohol does not sterilize, it sanitizes.

You should wait to post crappy technique until you learn that it is crappy.

This is misleading to new cultivators and you don't even know if it worked. A week is not long enough to see contams 95% of the time.

Best to not share "experimental" techniques until you get a harvest from it.

Welcome to the shroomery!




Hey buddy, don't be such a dick. I am fully aware that this was a ghetto technique...I expect high contamination rates. The point of the post is to illustrate that dried sclerotia remain viable, information which I could not find on this forum prior to posting this.

Edited by DDAWGG (09/20/13 09:21 AM)

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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: dried sclerotia as inocculant [Re: DDAWGG]
    #18866810 - 09/20/13 09:21 AM (10 years, 6 months ago)

Well don't post misleading information as a "tek" when you are expecting high contam rate.

You haven't been around here much but I have.

New members are going to see this post later on down the road and think it's a great idea. 

So I'm here to let them know it is not.

It's not personal. You just happen to be the one making the post this time.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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InvisiblebodhisattaMDiscordReddit
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Re: dried sclerotia as inocculant [Re: FrankHorrigan]
    #18866833 - 09/20/13 09:27 AM (10 years, 6 months ago)

Quote:

information which I could not find on this forum prior to posting this.




Either you're lying, and never touched the search engine, OR I think we need to make you a search engine TEK

http://www.shroomery.org/forums/showflat.php/Number/17012586#17012586

http://www.shroomery.org/forums/showflat.php/Number/11082150#11082150

http://www.shroomery.org/forums/showflat.php/Number/2700316#2700316

http://www.shroomery.org/forums/showflat.php/Number/12719885#12719885

Edited by Trusted cuItivator (09/20/13 09:28 AM)

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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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OG Cultivator
Re: dried sclerotia as inocculant [Re: DDAWGG]
    #18866834 - 09/20/13 09:27 AM (10 years, 6 months ago)

Quote:

DDAWGG said:

Hey buddy, don't be such a dick. I am fully aware that this was a ghetto technique...I expect high contamination rates. The point of the post is to illustrate that dried sclerotia remain viable, information which I could not find on this forum prior to posting this.




:facepalm:

It's a well-known fact that the evolutionary purpose of sclerotia is to allow the mycelium to dry totally out during adverse weather conditions and then rejuvenate once the weather returns to a more suitable state.

I and others have posted many threads showing how sclerotia can be used as inoculant for agar.  You cut from the center of a freshly broken piece of sclerotia to reduce the ambient contaminant load.  Once transferred, anything you see within 48 to 60 hours is a contaminant, so you're growing 100% contams.

It takes much longer than that for the dry sclerotia to hydrate and become active again.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineDDAWGG
Stranger
Registered: 09/20/13
Posts: 5
Last seen: 10 years, 5 months
Re: dried sclerotia as inocculant [Re: FrankHorrigan]
    #18866849 - 09/20/13 09:31 AM (10 years, 6 months ago)

Quote:

FrankHorrigan said:
Well don't post misleading information as a "tek" when you are expecting high contam rate.

You haven't been around here much but I have.

New members are going to see this post later on down the road and think it's a great idea. 

So I'm here to let them know it is not.

It's not personal. You just happen to be the one making the post this time.




Fair enough, I didn't view your comments from that perspective. I just wanted to provide insight as to how I went about things...

Frankly I'm surprised none of the jars (~15) are showing contamination yet...The mycellium growth from the sclerotias is vigorous. Being that any contams would likely be on the sclerotia itself, would they still pose a contamination risk after that region of the substrate is fully colonized by the culture of interest?

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InvisibleLeopardMan
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Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: dried sclerotia as inocculant [Re: DDAWGG] * 1
    #18866852 - 09/20/13 09:31 AM (10 years, 6 months ago)

A circle of candles?  :knowwhatnevermind:

You've stolen my idea man: sterilizing petri dishes with the help of 66 druids at Stonehenge.



--------------------



You have to die a few times before you can really live.

-Charles Bukowski-

Edited by LeopardMan (09/21/13 11:02 AM)

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OfflineDDAWGG
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Registered: 09/20/13
Posts: 5
Last seen: 10 years, 5 months
Re: dried sclerotia as inocculant [Re: bodhisatta]
    #18866856 - 09/20/13 09:33 AM (10 years, 6 months ago)


Quote:

bodhisatta said:
Quote:

information which I could not find on this forum prior to posting this.




Either you're lying, and never touched the search engine, OR I think we need to make you a search engine TEK

http://www.shroomery.org/forums/showflat.php/Number/17012586#17012586

http://www.shroomery.org/forums/showflat.php/Number/11082150#11082150

http://www.shroomery.org/forums/showflat.php/Number/2700316#2700316

http://www.shroomery.org/forums/showflat.php/Number/12719885#12719885




I used DRIED sclerotia. I found information about using fresh stones, but not dried ones.

Edited by DDAWGG (09/20/13 10:00 AM)

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OfflineRogerRabbitM
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Re: dried sclerotia as inocculant [Re: DDAWGG]
    #18866875 - 09/20/13 09:38 AM (10 years, 6 months ago)

Quote:

I used DRIED sclerotia.




I saw that the first time without the capitalization. 

Always use full sterile procedure in the future to have any chance for success.  You might also look into some antibiotic agar for use with dry cultures since they will naturally have a higher bacterial contaminant load.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineDDAWGG
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Registered: 09/20/13
Posts: 5
Last seen: 10 years, 5 months
Re: dried sclerotia as inocculant [Re: RogerRabbit]
    #18866884 - 09/20/13 09:40 AM (10 years, 6 months ago)

Quote:

RogerRabbit said:
Quote:

DDAWGG said:

Hey buddy, don't be such a dick. I am fully aware that this was a ghetto technique...I expect high contamination rates. The point of the post is to illustrate that dried sclerotia remain viable, information which I could not find on this forum prior to posting this.




:facepalm:

It's a well-known fact that the evolutionary purpose of sclerotia is to allow the mycelium to dry totally out during adverse weather conditions and then rejuvenate once the weather returns to a more suitable state.

I and others have posted many threads showing how sclerotia can be used as inoculant for agar.  You cut from the center of a freshly broken piece of sclerotia to reduce the ambient contaminant load.  Once transferred, anything you see within 48 to 60 hours is a contaminant, so you're growing 100% contams.

It takes much longer than that for the dry sclerotia to hydrate and become active again.
RR




well perhaps I have contaminations then...I'm gonna let it go for a while and keep an eye on it.
Quote:

RogerRabbit said:
Quote:

I used DRIED sclerotia.




I saw that the first time without the capitalization. 

Always use full sterile procedure in the future to have any chance for success.  You might also look into some antibiotic agar for use with dry cultures since they will naturally have a higher bacterial contaminant load.
RR



was replying to bodhisatta...his posts clearly indicate that there was no consensus on whether dried sclerotias are still viable.

I will do that next time and wash the stone with hydrogen peroxide...for now I'll let things rip, perhaps I'll get lucky and some of the jars won't contaminate.

Edited by DDAWGG (09/20/13 10:52 AM)

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