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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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Offlinekinkaku
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Re: Is pouring agar in a SAB safe? Doesn't the heat/steam cause all kins of airflow? [Re: Mush4Brains]
    #18865728 - 09/19/13 11:37 PM (10 years, 5 months ago)

Quote:

Mush4Brains said:
If you get a large 140ml+ syringe + an extra long needle you can do many plates at once.




never knew they made them that big :eek: (thats what she said)


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OfflineMush4Brains
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Re: Is pouring agar in a SAB safe? Doesn't the heat/steam cause all kins of airflow? [Re: kinkaku]
    #18865750 - 09/19/13 11:41 PM (10 years, 5 months ago)

Yeah, not only that, but then you can sterilize in a pint jar with SFD/Tyvek and a silicone port and just suck it all up after sterilizing without having to worry about not being sterile :trippinbawelz:


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Offlinekrypto2000
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Re: Is pouring agar in a SAB safe? Doesn't the heat/steam cause all kins of airflow? [Re: Mush4Brains]
    #18865789 - 09/19/13 11:49 PM (10 years, 5 months ago)

So you use the needle when 'pouring' the petris or do you take the needle off and just use the syringe? I thought it was the later, but now you guys are mentioning the needle.


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Offlinekinkaku
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Re: Is pouring agar in a SAB safe? Doesn't the heat/steam cause all kins of airflow? [Re: krypto2000]
    #18865796 - 09/19/13 11:53 PM (10 years, 5 months ago)

i do the needle because I do what mush4brains said and do my agar in a quart jar with filter patch and SHIP'S that way it stays nice and sterile.


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Offlinekrypto2000
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Re: Is pouring agar in a SAB safe? Doesn't the heat/steam cause all kins of airflow? [Re: kinkaku]
    #18865894 - 09/20/13 12:25 AM (10 years, 5 months ago)

Cool, well I'll try that next time then. I assume doing that I wouldn't really have much steam and thus wouldn't need to raise the lid either.


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Offlinekinkaku
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Re: Is pouring agar in a SAB safe? Doesn't the heat/steam cause all kins of airflow? [Re: krypto2000]
    #18865899 - 09/20/13 12:26 AM (10 years, 5 months ago)

:shrug: I've never had actual steam come off my agar other than the condensation on the lids after its poured.


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Offlinekrypto2000
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Re: Is pouring agar in a SAB safe? Doesn't the heat/steam cause all kins of airflow? [Re: kinkaku]
    #18865912 - 09/20/13 12:34 AM (10 years, 5 months ago)

Maybe I should have let it cool more? How long do you wait after the PCer is off and has released pressure? I took my dish out maybe 10-15 minutes after that and set it on the counter, waited maybe 20 minutes, so that the handle was cool enough to the touch, and then poured it.


Edited by krypto2000 (09/20/13 12:35 AM)


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Offlinekinkaku
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Re: Is pouring agar in a SAB safe? Doesn't the heat/steam cause all kins of airflow? [Re: krypto2000]
    #18865923 - 09/20/13 12:38 AM (10 years, 5 months ago)

I've never really timed it lol I just take the jar out of the pc when I can pick it up with a sock on my hand:shrug: then again I use the cheap one time use dishes so I dont have to sterilize those either.


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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag


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