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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: Beppe]
#18865929 - 09/20/13 12:41 AM (10 years, 4 months ago) |
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Anyways those cakes are at 12 days and no white growth spots at all near the injection ports.
I'm gonna do 2 control groups tomorrow. 1 jar steam sterilized and 1 jar unsterilized, and put them in the same room etc. No spores.
I'm curious. Either way I vow to isolate this problem. It's too systematic to occur in all 4 jars
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cynical bastad
another guy
Registered: 08/17/13
Posts: 175
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Re: I discovered new contam?? [Re: Beppe]
#18865960 - 09/20/13 12:52 AM (10 years, 4 months ago) |
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What about the possibility that your syringe is bad? Try a different one since you have it? I don't have answers, i have questions. Verm being wet and not being dry, due to taped up holes may be fighting against you as well?
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Beppe
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Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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I see no reason why the micropore tape would be a bad idea on only my jars. 2 of the 5 jars have no tape on their holes and only have the Verm layer. The Verm isn't the issue as these
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: I discovered new contam?? [Re: kinkaku]
#18866702 - 09/20/13 08:51 AM (10 years, 4 months ago) |
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Quote:
kinkaku said:


complements of beppe
That looks like wet BRF from your inoculant.
If you see slimy wet growth though, that is bacteria. Far from "new."
Quote:
Beppe said: Anyways those cakes are at 12 days and no white growth spots at all near the injection ports.
I'm gonna do 2 control groups tomorrow. 1 jar steam sterilized and 1 jar unsterilized, and put them in the same room etc. No spores.
What kind of test is that? Your unsterilized jar will contam, period. Just steam them both and inoculate one.
The problem sounds like a bad syringe.
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Beppe
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Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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The point of the experiment would be to see the unsterilized test jar contam with 0% chance of it being spore related and comparing the contam growth and distribution with my jars.
It makes plenty of sense if you think about it. If I put a jar with none of the same conditions and it also aquires this growth that is VERY relevant data to isolating the necessary variable.
I knocked up 4 new jars 2 days ago as I said earlier. 2 with the same syringe, and 2 with a new strain and syringe.
Right now I will tell you atleast 1 of the 4 jars has the early signs of this same growth. Wich does seem to be characteristic of the sub looking a little to wet. But it wasn't like that prior to 24 hours post innoc.
The 5 original jars have become colder and like 65% colonized by this opaque growth.
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Beppe
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Re: I discovered new contam?? [Re: Beppe]
#18866748 - 09/20/13 09:03 AM (10 years, 4 months ago) |
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Also the growth is not a slime. It's between cauliflower and over cooked rice. I even mashed some in mygloves one day and it was not slime at all.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: I discovered new contam?? [Re: Beppe]
#18866756 - 09/20/13 09:05 AM (10 years, 4 months ago) |
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I've been doing this for years. Trust me, it's probably a bad syringe.
What are you trying to "isolate" the "variable" for?
If your inoculant is bad, nothing will work.
When you have 100% failure rate with a single syringe, there is a 99% chance you have a contam'd syringe.
You won't be able to get much from it without agar.
What are you trying to accomplish?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: I discovered new contam?? [Re: Beppe]
#18866760 - 09/20/13 09:06 AM (10 years, 4 months ago) |
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Quote:
Beppe said: Also the growth is not a slime. It's between cauliflower and over cooked rice. I even mashed some in mygloves one day and it was not slime at all.
What you describe is either just wet BRF or bacteria.
Quote:


This is what you are talking about, correct?
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Beppe
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Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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I just didn't want to blame the issue being anything but my fault. If it's simply the syringe I will know within a week because 2 new jars are a different syringe/strain. Now if those get this same growth I'm back to attempting to isolate the variable.
But it can't be wet brf because of it's texture when pressed between glover fingers is too firm. It's got characteristics of something that was broken down by a biological process rather then moist grain.
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Beppe
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Re: I discovered new contam?? [Re: Beppe]
#18866788 - 09/20/13 09:17 AM (10 years, 4 months ago) |
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Yeah those are mine!
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: I discovered new contam?? [Re: Beppe]
#18866792 - 09/20/13 09:17 AM (10 years, 4 months ago) |
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Quote:
Beppe said: The point of the experiment would be to see the unsterilized test jar contam with 0% chance of it being spore related and comparing the contam growth and distribution with my jars.
There is zero chance a non-sterilized jar will do anything other than germinate and grow whatever has been in the air and on the grains or rice since before harvest. What grows has nothing to do with what would grow if the jar were sterilized and inoculated. RR
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Beppe
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Re: I discovered new contam?? [Re: RogerRabbit]
#18866813 - 09/20/13 09:22 AM (10 years, 4 months ago) |
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So my answers are: wet brf or unknown? This thread had nothing to do with my recently concocted test jars. It was about identifying the growth in my jars man?
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Beppe
Stranger
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Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: Beppe]
#18866822 - 09/20/13 09:24 AM (10 years, 4 months ago) |
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RR you just proved my point! Nothing will grow in the jars except whatever airborne contams are present and I need to know if there is some airborne variable that can cause this growth that no one has named or identified beyond "wet brf or idk contam"
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: I discovered new contam?? [Re: Beppe]
#18866846 - 09/20/13 09:30 AM (10 years, 4 months ago) |
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I don't think you quite understand the futility of leaving a jar unsterilized. There are millions of different bacteria species and mold spores in your air. What grows on your unsterilized cake will not tell you anything except that you now have a mess of different contams and can't tell them all apart.
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RogerRabbit
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Registered: 03/26/03
Posts: 42,214
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Re: I discovered new contam?? [Re: Beppe]
#18866850 - 09/20/13 09:31 AM (10 years, 4 months ago) |
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It doesn't matter. Nobody cares to even attempt to identify local spore loads. This is the reason we sterilize before starting. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: RogerRabbit]
#18866861 - 09/20/13 09:34 AM (10 years, 4 months ago) |
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Lmao "it doesn't matter, try new syringe and fuck off" alright thanks guys. Appreciate that.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: I discovered new contam?? [Re: RogerRabbit]
#18866878 - 09/20/13 09:39 AM (10 years, 4 months ago) |
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Quote:
RogerRabbit said: It doesn't matter. Nobody cares to even attempt to identify local spore loads. This is the reason we sterilize before starting. RR

contams are contams. If you want to try to grow a "new" contam, you are in the wrong place. We are here to help you grow mushrooms.
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Beppe said: Lmao "it doesn't matter, try new syringe and fuck off" alright thanks guys. Appreciate that.
Really? We're offering you advice that you can't get anywhere but here. Don't get angry because the advice you get is not the advice you want to hear.
Edited by FrankHorrigan (09/20/13 09:44 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: I discovered new contam?? [Re: Beppe]
#18866916 - 09/20/13 09:48 AM (10 years, 4 months ago) |
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Quote:
Beppe said: Lmao "it doesn't matter, try new syringe and fuck off" alright thanks guys. Appreciate that.
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: Pastywhyte]
#18867085 - 09/20/13 10:40 AM (10 years, 4 months ago) |
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Ahh! Im not angry! Sorry for the impression. That absolutely did come off as ungrateful.
All I was interested in was whether this is odd mycelium or an unknown contam If it is an unknown contam it should be documented because it looks mycelium like in some spots (rhizomorphic growth) and has next to no odor. That's all.
I see there has been tons of miscommunication here and no doubt that's partly my fault.
Thank you all for your help. You guys have taught me more then you know already, I was just looking for feedback and to possibly contribute to the contam log.
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: I discovered new contam?? [Re: Beppe]
#18868435 - 09/20/13 04:13 PM (10 years, 4 months ago) |
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Quote:
Beppe said: It makes plenty of sense if you think about it.
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