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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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I discovered new contam??
#18865225 - 09/19/13 09:17 PM (10 years, 4 months ago) |
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So I followed the PF tek perfect. Used a sanitized room with no air flow for over 5 hours. Then innoc'd and covered with micro tape (even though I had a dry Verm layer).
Within 24 hours my jars grew little gray white globs that were not cobweb at all. And looked nothing like bocculism. Within 5 days the jars were 25% colonized by these white cum looking blobs. Not creamy, not gooey. A consistency between jizz and over cooked rice spherical objects.
There was no foul odor and even a light mycelium smell. But those jars stalled and I tossed em. Using new jars I did 4 more and 1 already looks to be going this route.
What's going on I have researched this for months man. It's not any of the known contams, and I have had 0 mycelium appearance in 2 weeks of failed jars. I tried a different syringe, and doubled sanitation efforts. Now what?
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SgtGanja
CEO


Registered: 09/03/13
Posts: 134
Loc: United States
Last seen: 8 years, 10 months
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Re: I discovered new contam?? [Re: Beppe]
#18865234 - 09/19/13 09:19 PM (10 years, 4 months ago) |
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A picture is worth a thousand words
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: I discovered new contam?? [Re: SgtGanja]
#18865252 - 09/19/13 09:22 PM (10 years, 4 months ago) |
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you do know there are a couple different ways mycelium grows right? are you sure what you where seeing was indeed a contam?
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cgsjames
I eat dumbbells



Registered: 05/18/13
Posts: 178
Last seen: 12 days, 17 minutes
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Re: I discovered new contam?? [Re: SgtGanja]
#18865361 - 09/19/13 09:53 PM (10 years, 4 months ago) |
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Quote:
SgtGanja said: A picture is worth a thousand words 
Picture please?...
-------------------- What you eat don't make me shit and who you fuck don't make me come.
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: cgsjames]
#18865543 - 09/19/13 10:40 PM (10 years, 4 months ago) |
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I have pics but no way to upload them off iPhone. Anyone I can email and they'll post them? The lighting/camera enhance browns and yellows so the subs not as pale looking as it should be compared to the gnarly photos. But it captures the grey well.
Think if my substrate hade overcooked cauliflower in it. Very over cooked white circular membranes akin to the opaque cauliflowerettes that disconnect and overcook in the pan.
I took 1 jar and opened it outside. It's made colonized 25% of the sub so it's a cake now but very lacking in white hot spots typical in everyone's growth pics. This gray/white stuff is all Over and around the sub, not just localized to injection locations.
At first I thought I overfilled the Verm barrier and the spores were leaking down it on the sides and that could justify such non localized growth, but this stuffs grayish white and just appeared way too sudden in such spreaded patches in-between airier parts in the substrate.
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: Beppe]
#18865599 - 09/19/13 10:59 PM (10 years, 4 months ago) |
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Can some one please help me upload these pics since I'm on iPhone and can't upload
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: I discovered new contam?? [Re: Beppe]
#18865620 - 09/19/13 11:05 PM (10 years, 4 months ago) |
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you're new so I'm not sure if its a good idea asking that.
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myplague240


Registered: 03/17/13
Posts: 127
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: kinkaku]
#18865633 - 09/19/13 11:10 PM (10 years, 4 months ago) |
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Beppe, how are you sterilizing these jars? Also, you didn't tell us if you are using a S.A.B (Still air box) or a Gb (Glove box).
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: kinkaku]
#18865644 - 09/19/13 11:15 PM (10 years, 4 months ago) |
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Yeah I understand the implications but I'm pretty sure if there's a cauliflower contam that doesn't match any other white/grey contams this photo should be added to the common contam FAQ pics even if no one knows wtf it is or how I got it in 5 jars.
I'm telling you it's not any of the grey/white contams. This stuff looks like the white tissue that grows on wounds in your mouth. Not quite a cream, not quite a water logged rice grain.
No glove box yet! I just closed off air in a bathroom and had all fans off for hours, then slowly shifted into the room- slowly shut the door- and then waited a bit for air to slow down from the door closing. Then I knocked them jars up with Accidently too much MS syringe. My novice hands dosed out 1-1.5cc a jar
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: Beppe]
#18865656 - 09/19/13 11:18 PM (10 years, 4 months ago) |
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Oh I peroxide tested the one I opened to check for cobweb. Nada. It just got wet. Cut that cake up into pieces to investigate the interior and left them on the grass and nature ate it in 1 day
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: I discovered new contam?? [Re: Beppe]
#18865663 - 09/19/13 11:19 PM (10 years, 4 months ago) |
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eh, what the hell I'll pm you my email if no one else has yet
but....if the cops show up at my door I'll kill everyone with my fist and teeth.
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myplague240


Registered: 03/17/13
Posts: 127
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: Beppe]
#18865668 - 09/19/13 11:20 PM (10 years, 4 months ago) |
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Well before you proceed any further in this hobby with time and money I'd recommend investing in a SAB/GB and a pressure cooker. Also, BRF cakes are so prone to contamination issues that if you ever step up to grain you're going to wonder why you even messed with em'.
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: kinkaku]
#18865674 - 09/19/13 11:21 PM (10 years, 4 months ago) |
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*your going to jail now ad lib* thanks man. Keep in mind these pics are drier then they look.
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: Beppe]
#18865693 - 09/19/13 11:27 PM (10 years, 4 months ago) |
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I wish I could just do the PF tek with cior sub and nothing else. Would be so easy. Idk but the basic half pint pf resonates with me as the ultimate user experience for home lab cultivation of mushrooms. Speaking in quantum psychological terms, it gets me hard!
I want to master pf so I can move up to Spongis Pf Block tek. It just seems so easy when your procedures tight.
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myplague240


Registered: 03/17/13
Posts: 127
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: Beppe]
#18865732 - 09/19/13 11:37 PM (10 years, 4 months ago) |
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Well, Beppe, I'll tell you this.. when you invest in a pressure cooker and move up to quart jars with grains.. while using proper sterile techniques you will see little to no contam problems.
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: myplague240]
#18865763 - 09/19/13 11:43 PM (10 years, 4 months ago) |
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Look while their are fine techniques out there that one day I'll try out- I need to make these cakes work. Now I got plenty of Verm and BRF. and 2 and 1/2 syringes left. Which will for sure be made into liquid cultures also to keep the movement moving. This is too important to me. I need to make these cakes happen.
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: I discovered new contam?? [Re: Beppe]
#18865865 - 09/20/13 12:16 AM (10 years, 4 months ago) |
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complements of beppe
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: kinkaku]
#18865872 - 09/20/13 12:18 AM (10 years, 4 months ago) |
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Thanks man. Figures it would be you to help. I been laughing at that signature for days. Godamnit Carl Jung synchronistic occurrences.
But yeah the jars aren't wet or discolored in anyway beyond what the camera added
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: I discovered new contam?? [Re: Beppe]
#18865890 - 09/20/13 12:24 AM (10 years, 4 months ago) |
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Quote:
Beppe said: Thanks man. Figures it would be you to help. I been laughing at that signature for days. Godamnit Carl Jung synchronistic occurrences.
But yeah the jars aren't wet or discolored in anyway beyond what the camera added
haha no problem
where they like that out of the pc? a long time ago I did rye flour cakes and they turned out like bricks and looked like that kinda.
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Beppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
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Re: I discovered new contam?? [Re: kinkaku]
#18865908 - 09/20/13 12:30 AM (10 years, 4 months ago) |
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Nah man. The growth essentially began as singular blobs within 48 hours of knock up
My hypothesis are: A) bizarre mycelium B) unknown fungus infecting my air of the entire downstairs C) improper steam levels (too much escaping from the lid) and partial heat encouraging a fast unknown agent
Idk man. I'm pretty smart for a bohemian and I absolutely can rock out boomers. I oversaw a bag grow in Denmark once and only my bag colonized white (everyone else got tryc). So I def got the potential and ability to learn from Internet sources
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