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North Spore Shop: Cultivation Supplies, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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InvisibleSpitballJedi
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Registered: 10/13/12
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Agar technique
    #18863307 - 09/19/13 02:26 PM (10 years, 4 months ago)

This post is for posterity sake.

Do you tilt your agar dishes sideways when cutting, inoculating, or otherwise transferring?

With the dish laying flat, your hands and non-flame-sterilized part of your tools are over the dish.

Could this be a source of peoples ninja contams?


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Invisibletbagtag
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Re: Agar technique [Re: SpitballJedi]
    #18863564 - 09/19/13 03:46 PM (10 years, 4 months ago)

I tilt, but just because it's easier.


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OfflinePrinceShroom
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Re: Agar technique [Re: tbagtag]
    #18863607 - 09/19/13 03:58 PM (10 years, 4 months ago)

Quote:

tbagtag said:
I tilt, but just because it's easier.



:whathesaid:
Much easier


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WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineHofmann1943
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Re: Agar technique [Re: PrinceShroom]
    #18863630 - 09/19/13 04:04 PM (10 years, 4 months ago)

I do not, i do the last cut at the angle. It is easy to get it out that way and mu tool and specially hand are never over the plate.


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By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.


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OfflineDeadPhan
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Re: Agar technique [Re: Hofmann1943]
    #18864079 - 09/19/13 05:37 PM (10 years, 4 months ago)

I'm new to agar but working with jars.  I tried not to tilt, and didn't at first, but recently doing a transfer of wedges to grain there was no other way.


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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InvisibleSpitballJedi
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Re: Agar technique [Re: DeadPhan]
    #18864274 - 09/19/13 06:11 PM (10 years, 4 months ago)

I'm starting to wonder if there is any point in using IPA on my gloved hands.

I, and others, use soap water inside the SAB. I wonder if just using soap water on my gloves would actually be as good, if not better.

I know my SAB stays damp longer with soap water than with IPA.


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OfflinePocketRevolution
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Re: Agar technique [Re: SpitballJedi]
    #18865540 - 09/19/13 10:40 PM (10 years, 4 months ago)

I think you would find that using soap water on your gloves would make them very slippery, and you might have a hard time holding onto jars, opening them, etc.

I once tried using hand sanitizer gel on the gloves, figuring it would last longer.  Couldn't get a grip on the jar or lid, couldn't open the jars.  Fail.


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North Spore Shop: Cultivation Supplies, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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