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Invisibletbagtag
Boomer Barron

Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam Flag
Dumb question on agar wedge transfers
    #18863287 - 09/19/13 02:19 PM (10 years, 4 months ago)

What do I cool my scalpel on before transferring the wedge?  Just let it air cool?


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
Re: Dumb question on agar wedge transfers [Re: tbagtag]
    #18863291 - 09/19/13 02:20 PM (10 years, 4 months ago)

You should be able to just cut into the agar and that will cool the scalpel after the first cut.

Edit: Here is RR on the subject:
Quote:

RogerRabbit said:
I prefer to use the needle or scalpel as soon as it's 'cool' without wiping.  Normally, a drop or two of solution can be squeezed out from a red hot needle and it's ready to use.  If it's a scalpel, the fist swipe through the petri dish or mushroom flesh sizzles it enough to cool for a sterile transfer.




http://www.shroomery.org/forums/showflat.php/Number/8608785#8608785


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InvisibleSpitballJedi
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Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Dumb question on agar wedge transfers [Re: tbagtag]
    #18863321 - 09/19/13 02:31 PM (10 years, 4 months ago)

If it's a wedge, like for transferring to grain, there will be plenty of healthy culture for your transfer if you just stick your hot scalpel in.

If you are just trying to get a small sample, like when isolating, I stick the hot scalpel in the edge of the agar where there is no growth.


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Invisibletbagtag
Boomer Barron

Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam Flag
Re: Dumb question on agar wedge transfers [Re: SpitballJedi]
    #18863328 - 09/19/13 02:32 PM (10 years, 4 months ago)

Thank you both, this is only my second agar to grain transfer.  The first one I just transferred the whole sector and waited for the scalpel to cool.  Now I have whole petri's that can be cut.  Thanks again guys!

Mush - 5 shrooms to you.

Spit, since you opted out this is the best I can do: :boobs:


Edited by tbagtag (09/19/13 02:34 PM)


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