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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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SAB procedure and Agar Question
#18859687 - 09/18/13 06:38 PM (10 years, 5 months ago) |
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Three somewhat unrelated questions:
1.) In wiping down the SAB with soapy water, after your done with your SAB work do you need to clean this soapy water out or can you just let the water evaporate? I've heard people say they never clean their SAB, but I don't know if they're using a different procedure than the soapy water or what. I'd rather not if I don't need to since I'd have to take it into the bathtub or use the outside hose because it's so big and then set it somewhere to dry.
2.) Using plastic disposable petris (not sure if glass are any different) should you tape down the lid after inoculating them or just let it rest on top? I'm concerned contaminants can get in without taping it, but I don't know, likewise will taping it prevent/limit GE too much?
3.) How do you store premade agar dishes before using them and how long are they typically good for? I assume they'll be fine unrefrigerated?
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bulkgrownoob
Registered: 03/21/11
Posts: 345
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Re: SAB procedure and Agar Question [Re: krypto2000]
#18859802 - 09/18/13 07:01 PM (10 years, 5 months ago) |
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1. Why not just wipe it out with a towel? It doesn't need to literally be cleaned afterwards but drying it isn't going to hurt anything.
2. Wrap parafilm around the opening to allow GE
3. Depends how deep you pour your agar which will determine how long it will take to dry out. The more agar the longer it should last. And room temp or the fridge is fine, doesn't really matter until you knock it up
Edited by bulkgrownoob (09/18/13 07:01 PM)
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krypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Re: SAB procedure and Agar Question [Re: bulkgrownoob]
#18859810 - 09/18/13 07:04 PM (10 years, 5 months ago) |
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Quote:
bulkgrownoob said: 2. Wrap parafilm around the opening to allow GE
I don't have any for the time being and have already knocked up some dishes. Can you not just use the lid? Is the lid only to keep contaminants out before you use it then?
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bulkgrownoob
Registered: 03/21/11
Posts: 345
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Re: SAB procedure and Agar Question [Re: krypto2000]
#18859858 - 09/18/13 07:13 PM (10 years, 5 months ago) |
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Just found this post by RR a while back
Quote:
RogerRabbit said: Yes, for those of us without sterile, climate controlled labs to store culture dishes in, use parafilm and then insert each petri dish into a brand new ziplock baggie which come sterile out of the box. If you do this in front of a flowhood, you can inflate the baggie with sterile air before sealing.
Not only does the baggie help prevent contamination and drying of the culture, it keeps the mold or whatever contained to the individual dish that turns out to be contaminated. RR
If you don't have parafilm I guess a ziplock alone should be better than nothing.
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TheApprentice
back at it



Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 2 months
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Re: SAB procedure and Agar Question [Re: bulkgrownoob]
#18859874 - 09/18/13 07:17 PM (10 years, 5 months ago) |
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That glad press and seal works in place of parafilm.
op. Put the lid on. Wrap the press and seal around the edge where the top and bottom meet. Around the circumference...
--------------------
RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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coffeehead
Perpetual Learner

Registered: 01/15/13
Posts: 142
Last seen: 5 years, 2 months
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Re: SAB procedure and Agar Question [Re: bulkgrownoob]
#18859948 - 09/18/13 07:34 PM (10 years, 5 months ago) |
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1) I use Lysol wipes on everything. Plus a spritz or three of 10% Clorox water that I just let dry. No contamination problems.
2) There are two dish designs -- one has a flush fitting lid, the other has ridges that create a gap for air exchange. Contaminants will get in with the air on this type. Parafilm is wicked expensive, but if you have dishes designed with a gap it's recommended. If you can keep your dishes in a sealed, sanitized tub you might not need the film. As long as the air is still and relatively clean there's a good chance contaminants won't get in but you'll be taking a risk. Even a small tub will give you plenty of air for FAE. That's what I do and so far I haven't gotten any contaminants. As soon as you start moving them, though, you'll stir up the air so tape them closed if you're going to move them.
3) Store your plates in the refrigerator, upside down.
I bought some pre-poured PDA plates from one of the major lab supply companies. The directions said to store them in a refrigerator upside down. Otherwise you'll get condensation on the agar. I blew it and read the instructions three days after putting them in the fridge and the top four plates had condensation on the agar. Also, keep them in a clean ziploc or tub.
Edit: I just saw the glad press and seal suggestion. That's a great idea I'll be using in the future.
Edited by coffeehead (09/18/13 07:37 PM)
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