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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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secon opinion on my agar cultures
#18859548 - 09/18/13 06:05 PM (10 years, 4 months ago) |
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ok so I took a look at these the other day and figured it was thin mycelium due to the low amount of agar (about 9ml). So I transfered away onto new dishes. well now today whenever I look at them for some reason it just doesnt seem right to me . This isn't my first rodeo but I've never seen it thin like this. any second opinions would be much obliged
p.s: I already know about the bacteria
the agar is just your average run of the mill LME,9ml in each dish. the tissue itself is cubensis (albino a+).


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PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
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Re: secon opinion on my agar cultures [Re: kinkaku]
#18859578 - 09/18/13 06:11 PM (10 years, 4 months ago) |
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There is bac in the middle of those plates too your gonna have to keep transfering til you clean it up
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  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: secon opinion on my agar cultures [Re: PrinceShroom]
#18859598 - 09/18/13 06:14 PM (10 years, 4 months ago) |
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haha I already stated that I knew about the bacteria but thanks
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: secon opinion on my agar cultures [Re: kinkaku]
#18863459 - 09/19/13 03:16 PM (10 years, 4 months ago) |
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i know its against the rules to bump before 24 hours but i just want to get this up there before most the knowledgable people go to bed
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provo
Peace Pilgrim

Registered: 10/18/09
Posts: 189
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Re: secon opinion on my agar cultures [Re: kinkaku]
#18863721 - 09/19/13 04:26 PM (10 years, 4 months ago) |
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I know you have stated you are aware of the bacterial infections present but do keep in mind the mycelium can be affected by this "competition" so that may be why you are seeing poor/strange growth.
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: secon opinion on my agar cultures [Re: provo]
#18863955 - 09/19/13 05:06 PM (10 years, 4 months ago) |
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hmm, what are we asking here? are we asking why the mycelia is like a thin cotton candy wisp?

i think , you crave a ravenous agar.
something thick, that climbs the walls ?......

the type of stuff that makes ultra rhizoids?

something that transfers heavy and thick? with fast recovery response?

i think i know what ya need. but first, lets see what the others have to say.....

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Edited by anne halonium (09/19/13 05:16 PM)
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: secon opinion on my agar cultures [Re: anne halonium]
#18863985 - 09/19/13 05:15 PM (10 years, 4 months ago) |
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Quote:
anne halonium said: hmm, what are we asking here? are we asking why the mycelia is like a thin cotton candy wisp?

well I geuss I just wanted to see what you guys thought about it seems a little off to me.
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: secon opinion on my agar cultures [Re: kinkaku]
#18863997 - 09/19/13 05:17 PM (10 years, 4 months ago) |
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obviously, ya would want a more responsive agar...... lets see what others suggest first.......
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: secon opinion on my agar cultures [Re: anne halonium]
#18864103 - 09/19/13 05:42 PM (10 years, 4 months ago) |
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you remind me of a venetian plague doctor haha. what is this magic you speak of?
also are you doing cultures in glass milk bottles?
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: secon opinion on my agar cultures [Re: anne halonium]
#18864119 - 09/19/13 05:45 PM (10 years, 4 months ago) |
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Quote:
anne halonium said: hmm, what are we asking here? are we asking why the mycelia is like a thin cotton candy wisp?

What is that housing the agar?
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: secon opinion on my agar cultures [Re: DeadPhan]
#18864157 - 09/19/13 05:52 PM (10 years, 4 months ago) |
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: secon opinion on my agar cultures [Re: anne halonium]
#18864159 - 09/19/13 05:53 PM (10 years, 4 months ago) |
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Quote:
Whippy said:
Quote:
DeadPhan said: What is that housing the agar?
Culture bottle from the previous mentions.
http://www.carlroth.com/catalogue/catalogue.do;jsessionid=63FEB33B8D4670301CAD1441EDD5961E?favOid=000000000001c10f00020023&act=showBookmark&lang=en-com&market=COM
Something like above would be my best guess.
thos culture bottles are amazing haha to bad they're pretty expensive
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abltsandwich
JFK = Jelly Donut




Registered: 06/16/09
Posts: 11,537
Loc: Dildoville
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Re: secon opinion on my agar cultures [Re: kinkaku]
#18864244 - 09/19/13 06:05 PM (10 years, 4 months ago) |
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I do like those culture bottles we worked with them in several labs in school, especially Cell Bio.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: secon opinion on my agar cultures [Re: abltsandwich]
#18865849 - 09/20/13 12:09 AM (10 years, 4 months ago) |
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Those culture bottles are for plant tissue culture!
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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Re: secon opinion on my agar cultures [Re: inski]
#18868935 - 09/20/13 06:15 PM (10 years, 4 months ago) |
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bump ok does it look like a contam to anyone?
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