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Offlinekinkaku
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secon opinion on my agar cultures
    #18859548 - 09/18/13 06:05 PM (10 years, 4 months ago)

ok so I took a look at these the other day and figured it was thin mycelium due to the low amount of agar (about 9ml). So I transfered away onto new dishes. well now today whenever I look at them for some reason it just doesnt seem right to me :shrug:. This isn't my first rodeo but I've never seen it thin like this. any second opinions would be much obliged:thumbup:

p.s: I already know about the bacteria:thumbup:

the agar is just your average run of the mill LME,9ml in each dish.
the tissue itself is cubensis (albino a+).







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OfflinePrinceShroom
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Re: secon opinion on my agar cultures [Re: kinkaku]
    #18859578 - 09/18/13 06:11 PM (10 years, 4 months ago)

There is bac in the middle of those plates too your gonna have to keep transfering til you clean it up


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Offlinekinkaku
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Re: secon opinion on my agar cultures [Re: PrinceShroom]
    #18859598 - 09/18/13 06:14 PM (10 years, 4 months ago)

haha I already stated that I knew about the bacteria:thumbup: but thanks :cool:


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Offlinekinkaku
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Re: secon opinion on my agar cultures [Re: kinkaku]
    #18863459 - 09/19/13 03:16 PM (10 years, 4 months ago)

i know its against the rules to bump before 24 hours but i just want to get this up there before most the knowledgable people go to bed


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Invisibleprovo
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Re: secon opinion on my agar cultures [Re: kinkaku]
    #18863721 - 09/19/13 04:26 PM (10 years, 4 months ago)

I know you have stated you are aware of the bacterial infections present but do keep in mind the mycelium can be affected by this "competition" so that may be why you are seeing poor/strange growth.


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Invisibleanne halonium
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Re: secon opinion on my agar cultures [Re: provo]
    #18863955 - 09/19/13 05:06 PM (10 years, 4 months ago)

hmm, what are we asking here?
are we asking why the mycelia is like a thin cotton candy wisp?




i think , you crave a ravenous agar.

something thick, that climbs the walls ?......




the type of stuff that makes ultra rhizoids?




something that transfers heavy and thick?
with fast recovery response?




i think i know what ya need.
but first, lets see what the others have to say.....











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:aliendance:


Edited by anne halonium (09/19/13 05:16 PM)


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Offlinekinkaku
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Re: secon opinion on my agar cultures [Re: anne halonium]
    #18863985 - 09/19/13 05:15 PM (10 years, 4 months ago)

Quote:

anne halonium said:
hmm, what are we asking here?
are we asking why the mycelia is like a thin cotton candy wisp?







well I geuss I just wanted to see what you guys thought about it:shrug: seems a little off to me.


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Invisibleanne halonium
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Re: secon opinion on my agar cultures [Re: kinkaku]
    #18863997 - 09/19/13 05:17 PM (10 years, 4 months ago)

obviously, ya would want a more responsive agar......
lets see what others suggest first.......


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Offlinekinkaku
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Re: secon opinion on my agar cultures [Re: anne halonium]
    #18864103 - 09/19/13 05:42 PM (10 years, 4 months ago)

you remind me of a venetian plague doctor haha.
what is this magic you speak of?

also are you doing cultures in glass milk bottles?


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OfflineDeadPhan
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Re: secon opinion on my agar cultures [Re: anne halonium]
    #18864119 - 09/19/13 05:45 PM (10 years, 4 months ago)

Quote:

anne halonium said:
hmm, what are we asking here?
are we asking why the mycelia is like a thin cotton candy wisp?








What is that housing the agar?


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InvisibleHypnotoadCroaked
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Re: secon opinion on my agar cultures [Re: DeadPhan]
    #18864157 - 09/19/13 05:52 PM (10 years, 4 months ago)

Quote:

DeadPhan said:
What is that housing the agar?



Culture bottle from the previous mentions.

http://www.carlroth.com/catalogue/catalogue.do;jsessionid=63FEB33B8D4670301CAD1441EDD5961E?favOid=000000000001c10f00020023&act=showBookmark&lang=en-com&market=COM

Something like above would be my best guess.


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Offlinekinkaku
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Re: secon opinion on my agar cultures [Re: anne halonium]
    #18864159 - 09/19/13 05:53 PM (10 years, 4 months ago)



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Offlinekinkaku
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Re: secon opinion on my agar cultures [Re: HypnotoadCroaked]
    #18864168 - 09/19/13 05:54 PM (10 years, 4 months ago)

Quote:

Whippy said:
Quote:

DeadPhan said:
What is that housing the agar?



Culture bottle from the previous mentions.

http://www.carlroth.com/catalogue/catalogue.do;jsessionid=63FEB33B8D4670301CAD1441EDD5961E?favOid=000000000001c10f00020023&act=showBookmark&lang=en-com&market=COM

Something like above would be my best guess.




thos culture bottles are amazing haha to bad they're pretty expensive


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Invisibleabltsandwich
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Re: secon opinion on my agar cultures [Re: kinkaku]
    #18864244 - 09/19/13 06:05 PM (10 years, 4 months ago)

I do like those culture bottles we worked with them in several labs in school, especially Cell Bio.


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InvisibleinskiM
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Re: secon opinion on my agar cultures [Re: abltsandwich]
    #18865849 - 09/20/13 12:09 AM (10 years, 4 months ago)

Those culture bottles are for plant tissue culture!


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Offlinekinkaku
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Re: secon opinion on my agar cultures [Re: inski]
    #18868935 - 09/20/13 06:15 PM (10 years, 4 months ago)

bump ok does it look like a contam to anyone?


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