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OfflineCircle K
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Registered: 05/27/13
Posts: 127
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Circle K's WBS Exploits (PICS)
    #18855152 - 09/17/13 07:40 PM (10 years, 5 months ago)

I posted here a while back when cultivation was a pipe dream. I told you guys I was a slave laborer for a small Pomeranian with a coke problem and a handgun. Since then, the only change is we've got our shit together a bit more.

I picked up some 10CC syringes of both Hillbilly and Tasmanian. Followed Doc's WBS Tek, but without a PC, I just boiled 4 jars at a time for 90 mins. Took 'em out, shook 'em, and let them dry. This was all in the morning, so I innoc'd at night. I did twelve jars, with six receiving one CC of each respective strain. Shook those syringes, just to be sure. Innoc'd through a damp iso sponge, with no heat sterilization, into a nail-sized, polyfill filled hole.

I know you guys think the dog thing is a joke, but that son of a bitch shot my boy Robbie in the shoulder when he asked about air exchange, and I'm not about to lose an arm, too. We have our jars sitting on a black garbage bag, with another over them, inside a plastic, unholed, partially translucent Rubbermaid box. We have a small space heater pointed at the box on one end, with a heat lamp overlooking it. The box gets pretty warm, but the jars seem fine. All of this is in a walk-in closet, on the upper shelf. Both doors are closed, with a HEPA running through one of the doors, pointed inside. We know the air exchange works because the room got pretty warm before we vented the rest out. The closet is used for clothing storage, but not laundry.

Does all of that sound a bit right? I'm not about to take any pictures because that fucking dog would blow my brains out, but I'm just trying to make sure everything's fine. When should I first check, if inoculating happened this last Sunday night?


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



Edited by Circle K (09/28/13 06:18 PM)


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Invisibletbagtag
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Registered: 01/16/13
Posts: 1,432
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855163 - 09/17/13 07:43 PM (10 years, 5 months ago)

Man, with that killer setup you should be ready to fruit in days.


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Invisiblemaddchef
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Re: WBS Jars inoculated, now for the meantime.. [Re: tbagtag]
    #18855185 - 09/17/13 07:48 PM (10 years, 5 months ago)

Lol the shit is deep in here tbag


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibletbagtag
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Posts: 1,432
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Re: WBS Jars inoculated, now for the meantime.. [Re: maddchef]
    #18855192 - 09/17/13 07:50 PM (10 years, 5 months ago)

Did OP spawn already?  I missed that part.


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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855196 - 09/17/13 07:51 PM (10 years, 5 months ago)

Quote:

Circle K said:
without a PC, I just boiled 4 jars at a time for 90 mins




You'll be getting ounces for sure.

Quote:

We have a small space heater pointed at the box on one end, with a heat lamp overlooking it. The box gets pretty warm, but the jars seem fine.




I know that cubes prefer to colonize in the mid 70s but 90+ should work fine too, I bet they'll go twice as fast.



Edited by Trusted cuItivator (09/17/13 07:54 PM)


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OfflinePrinceShroom
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18855224 - 09/17/13 07:59 PM (10 years, 5 months ago)

Im glad you did your research cause this is an epic grow.  I totally understand why you arent posting pics.
:failboat:

You should use that handgun
:suicide:


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineCircle K
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Registered: 05/27/13
Posts: 127
Loc: Israeli Province of Detro...
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Re: WBS Jars inoculated, now for the meantime.. [Re: tbagtag]
    #18855330 - 09/17/13 08:22 PM (10 years, 5 months ago)

I forgot to mention that the endpoint is spawning these into 5050 Monotubs. I've done the research, but I have a game plan for even if one or two come out with no contams. At least one is gonna sit around and become a grain master, and I've got smaller containers for if I only get a few pint jars' worth.

I know that on this site there's really nothing to worry about, but I just want to keep my head down. There's no definite for how many shroomerites get busted out of the whole, but I'm certainly not risking my ass. Not to mention that dog likes watching me use my computer, and while he can read really well, this time I'm just watching him lick his own asshole.

I can give you guys pictures of the dog, at least, just not with his coke or his handgun. That would be illegal. :frown:

Quote:

tbagtag said:
Did OP spawn already?  I missed that part.




Yeah, I've inoculated the jars, and they're sitting in my closet now. While they're innoculating, how much should I worry about FAE? The scientific part of my brain wants to cut off all airflow to the box and let the birdseed get colonized on its own, but I can't be sure, so I didn't tape the box off. I Lysol the FUCK out of that closet a handful of times a day, just to be sure. Blows back into the room and makes it smell like a hospital.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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OfflinePrinceShroom
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855372 - 09/17/13 08:30 PM (10 years, 5 months ago)

If you did your research you missed the part where you cant boil grain jars, only cakes.
Steam sterilization will not work for grain.


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineCircle K
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Registered: 05/27/13
Posts: 127
Loc: Israeli Province of Detro...
Last seen: 10 months, 14 days
Re: WBS Jars inoculated, now for the meantime.. [Re: PrinceShroom]
    #18855486 - 09/17/13 09:00 PM (10 years, 5 months ago)

Quote:

PrinceShroom said:
If you did your research you missed the part where you cant boil grain jars, only cakes.
Steam sterilization will not work for grain.




Yeah, I figured that. I did enough research and read all about endospores, but I've read a few posts that tell me that while there is still a high contam chance, there is still possibility for successful fruiting, and to get to my next step, a couple/few jars is really all I need. If/When the jars contaminate, I'll just resterilize them and wait until my financial situation allows me to buy a pressure cooker. There's a 22 quart one up on ebay for about 110 bucks right now, I'm eyeing up that one.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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Invisiblemaddchef
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Posts: 5,602
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855619 - 09/17/13 09:32 PM (10 years, 5 months ago)

Without being a total Dick here......do some more research and keep it so simple you think it ain't work, seriously.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineCircle K
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Registered: 05/27/13
Posts: 127
Loc: Israeli Province of Detro...
Last seen: 10 months, 14 days
Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855991 - 09/17/13 10:58 PM (10 years, 5 months ago)

I DID do my research, you dunces. I've read a few threads where people boiling WBS have had success, so I'm understanding that while the majority of my jars may come out contaminated, I'm still working with the tools at hand to start producing for myself before I get a PC. It's funny that this is the second or third time I've had to bring this up.

I have trouble reading sarcasm through the internet, but thanks to whoever's genuinely advising me, here. My heater got the Rubbermaid box pretty warm, but definitely not 100. It's Tuesday night where I'm at, and figuring I innoc'd on Sunday, I haven't seen any growth, at all. I shook a few jars (Can I do that?) In my incubator, and there's nothing I can see. Again, I've got 6 Hillbilly, and 6 Tasmanian. I like the both so much that I'm gonna try half and half in a few tubs later on down the line, to see if there are any good results.

So let's bear in mind that I know it was a fuck-up to not PC my jars. Whoopee, unless you guys want to take your dickery further and just keep poking at it.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisibleSteyner
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Registered: 08/19/13
Posts: 85
Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18856649 - 09/18/13 03:12 AM (10 years, 5 months ago)

Too hot will just encourage bacterial growth dude. You want high 70s, 80 max. Which is room temperature in most parts. And you dont need the dark. these babies need gas exchange so don't tape up you box and bags. If you are using the bags to hide them fine, but really you just want them out on the shelf. And no more need for lysol man, they got the polyfil so no germs will get in from outside.


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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Steyner]
    #18857450 - 09/18/13 11:04 AM (10 years, 5 months ago)

I'm only staying for the lulz. There's no hope nor suggestions that will be of help or even listened to for that matter. Between the incubator, lysol, lack of PC, and the 10 other things that CircleK has actually said he did that will fail his grow I personally can't wait.

Where are those dog pictures?


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OfflineCircle K
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Registered: 05/27/13
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18859967 - 09/18/13 07:39 PM (10 years, 5 months ago)

Quote:

Steyner said:
Too hot will just encourage bacterial growth dude. You want high 70s, 80 max. Which is room temperature in most parts. And you dont need the dark. these babies need gas exchange so don't tape up you box and bags. If you are using the bags to hide them fine, but really you just want them out on the shelf. And no more need for lysol man, they got the polyfil so no germs will get in from outside.




For being one of the first people to actually make a suggestion, thanks.

I moved them to just being out in my upper closet, with a HEPA running on low, through it. I just had it figured they wouldn't need light nor gas, due to the mycelium needing to spread, but I'll take your word on this. Removed the heater and the lamp. All it really took was someone to tell me what I need to change around, you guys. The last part of Doc's tek said to incubate at 80, so that's what I figured. I tested a jar with a thermometer and had the ballpark of the mid-eighties with the heater and lamp on them (We fine tuned the fuck out of that setup), but if it's gonna fuck up my colonizing, fixing it up is easy.

When my jars colonize, I'll let you guys know.


Quote:

bodhisatta said:
I'm only staying for the lulz. There's no hope nor suggestions that will be of help or even listened to for that matter. Between the incubator, lysol, lack of PC, and the 10 other things that CircleK has actually said he did that will fail his grow I personally can't wait.




So, does most of this board just try to act like smart-asses? Does it make your E-peen feel any bigger? I never said I wasn't gonna change shit around, on account of someone just having made a suggestion, rather than some smart-assed comment.

I'm really trying to do this here, you guys. I'm taking every suggestion I can get. I'm trying to be respectful and if I didn't need help I wouldn't come here, but you guys just seem to be dishing out arrogance. It's not that I can't take it, that's no problem, it's that I'm genuinely trying to get shit done.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisibleSteyner
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18864037 - 09/19/13 05:27 PM (10 years, 5 months ago)

No sweat man. These guys are just giving you a hard time because there is a general concensus of how grains should be done for maximum effeciency, and although you mentioned docs tek you seem to be following none of the rules. :-) anyway i sincerely hope at least one of the dozen make it so you can move on to 1. First make a grain liquid culture to ensure 10 more syringes if that is an option for you then 2. Once recovered transfer to properly sterilized and hydrated grain jars to get to unlimited mycelium for life.

Its been four days now rite, any small white spots on those grains yet? Should be close if not there yet. Although can take up to three weeks depending on the quality of your ms syringes. Expect the jars you shook to to take longer than the others, shaking before you see any strong growth is strongly advised against. You want to shake once you got nice strong growth, size of a golfball maybe. How is the moisture content looking in those babies? If you soaked and dried well they should look damp, not wet. Dont worry about condensation if you have those at the tops, at the heat you had them on i suspect you have a lot. However its just a nuisance, not the end of the world. At normal temps like you have now it should dissappear in a day or so.

Next time flame sterilize the needle before and between each jar. Sure you can get a lighter or a fking match for that matter? And dont try to resterilize jars that have contamed man, chuck the grain and restart. Try having a bigger size poly filled hole per jar too, or if only nails available, make three or four holes in each jar. Will allow for better gas exchange and faster growth. Just dont fuck with the inoculated jars now, have patience and let me know how it turned out!


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Steyner]
    #18867381 - 09/20/13 12:03 PM (10 years, 5 months ago)

Quote:

Steyner said:
No sweat man. These guys are just giving you a hard time because there is a general concensus of how grains should be done for maximum effeciency, and although you mentioned docs tek you seem to be following none of the rules. :-) anyway i sincerely hope at least one of the dozen make it so you can move on to 1. First make a grain liquid culture to ensure 10 more syringes if that is an option for you then 2. Once recovered transfer to properly sterilized and hydrated grain jars to get to unlimited mycelium for life.

Its been four days now rite, any small white spots on those grains yet? Should be close if not there yet. Although can take up to three weeks depending on the quality of your ms syringes. Expect the jars you shook to to take longer than the others, shaking before you see any strong growth is strongly advised against. You want to shake once you got nice strong growth, size of a golfball maybe. How is the moisture content looking in those babies? If you soaked and dried well they should look damp, not wet. Dont worry about condensation if you have those at the tops, at the heat you had them on i suspect you have a lot. However its just a nuisance, not the end of the world. At normal temps like you have now it should dissappear in a day or so.

Next time flame sterilize the needle before and between each jar. Sure you can get a lighter or a fking match for that matter? And dont try to resterilize jars that have contamed man, chuck the grain and restart. Try having a bigger size poly filled hole per jar too, or if only nails available, make three or four holes in each jar. Will allow for better gas exchange and faster growth. Just dont fuck with the inoculated jars now, have patience and let me know how it turned out!




I appreciate all that. I tried to follow it as best as I can, but I know I put a lot of my own spins on it. More than one jar has these beautiful little white spots, and they certainly don't look like trich, so that's a total plus. I will have to look up liquid cultures and how to make them; I've heard the term, but I haven't looked into it much. I had grain masters in my plans, but that sounds a ton more efficient. I know it's a push, but do you know if I would have any luck transferring the birdseed to a more forgiving substrate? Yield doesn't matetr so much to me, as much as making sure I don't have to worry about contamination.

It's been four days, yeah. I inoculated a few jars on Saturday, but those aren't showing anything. I've got spots on more than one jar, and I'm gonna leave those aside. I shook one before reading this, but that was only because I was searching for growth on the inside. As soon as that myc gets nice and fat, we'll facilitate redistribution. I'm actually pretty excited for this! I'm a total noob I know, but getting those spots tells me that I've done right enough.

When I reinnoculated my jars I did it near the stove, and got those syringes red hot. I wanted to make sure the only thing I did wrong that time was boiling - Each jar has a 1/4th inch hole stuffed with tyvek, with two coffee filters rubber banded to the top. We got the holes that wide by being nice and resourceful, (Getting really stoned and using screws to get some girth up in that bitch), but now we're just gonna let them sit for a few days. Seeing these white spots, I know that there's nothing I'll need to add to these jars to keep growth up, and I'm a bit intimidated that anything I try to fix on them will contaminate them.

I really want to upload some pictures you guys, but you have to understand the whole paranoia thing, about putting pictures of that on the internet, and shit. I know tens of dozens of people post them on a daily basis, but is it dangerous here, if at all?


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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OfflinePrinceShroom
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18868288 - 09/20/13 03:42 PM (10 years, 5 months ago)

Id say shroomery is one of the safest places to post TBH.  They strip all EXIF data fr all pics.


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineCircle K
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Registered: 05/27/13
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18885250 - 09/24/13 02:40 PM (10 years, 4 months ago)

Hey guys, checking back in.

An EXIF scrubber would be just what I need. I use an older digital camera that probably doesn't even have data like that, but my general interest is covering every base.

Anyway, I'm uploading these couple pictures of my best jars. I'm picking up some verm and coir later on, and when I'm able to, I'm gonna grab some BRF and a couple dozen more jars. The PC comes later on, probably next month, on account of me being done with my hefty bag of dried WBS for the meantime!

Anyway, these jars look great, could someone give me a rule of thumb on shaking them? One's got a bottom that's awesome, and going off the golf ball rule, I'd say at least one's ready. I'm most likely gonna shake it when I get home tonight, to give it that little extra edge.

One of the jars has an icky dark-brown, well, I don't know what it is at the bottom, I'll get a picture later on. Our first jar, colonizing way before the others, developed a nasty clump of mold, but that's what we've been ready for, having not PC'd grains, although I'm still blown away that these jars are doing so good!





--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18886136 - 09/24/13 06:22 PM (10 years, 4 months ago)

I'm no expert but all of those jars look bad maybe 1 of them is good. The coffee filters don't help anything.


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OfflineCircle K
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Registered: 05/27/13
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18886322 - 09/24/13 07:18 PM (10 years, 4 months ago)

Quote:

bodhisatta said:
I'm no expert but all of those jars look bad maybe 1 of them is good. The coffee filters don't help anything.




What do you mean? After I noc'd them up I shook each jar, which would explain the erratic growing. My camera may have not gotten them enough, but smell-wise, we're not getting anything nasty off of any of them.

I know I'm probably asking some elementary questions, here, but would it be ill-advised to g2g any one of these jars to a different substrate? I'm making the switch to PF-Style jars and I want to make sure they actually colonize.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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