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OfflineCircle K
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Registered: 05/27/13
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Circle K's WBS Exploits (PICS)
    #18855152 - 09/17/13 07:40 PM (10 years, 4 months ago)

I posted here a while back when cultivation was a pipe dream. I told you guys I was a slave laborer for a small Pomeranian with a coke problem and a handgun. Since then, the only change is we've got our shit together a bit more.

I picked up some 10CC syringes of both Hillbilly and Tasmanian. Followed Doc's WBS Tek, but without a PC, I just boiled 4 jars at a time for 90 mins. Took 'em out, shook 'em, and let them dry. This was all in the morning, so I innoc'd at night. I did twelve jars, with six receiving one CC of each respective strain. Shook those syringes, just to be sure. Innoc'd through a damp iso sponge, with no heat sterilization, into a nail-sized, polyfill filled hole.

I know you guys think the dog thing is a joke, but that son of a bitch shot my boy Robbie in the shoulder when he asked about air exchange, and I'm not about to lose an arm, too. We have our jars sitting on a black garbage bag, with another over them, inside a plastic, unholed, partially translucent Rubbermaid box. We have a small space heater pointed at the box on one end, with a heat lamp overlooking it. The box gets pretty warm, but the jars seem fine. All of this is in a walk-in closet, on the upper shelf. Both doors are closed, with a HEPA running through one of the doors, pointed inside. We know the air exchange works because the room got pretty warm before we vented the rest out. The closet is used for clothing storage, but not laundry.

Does all of that sound a bit right? I'm not about to take any pictures because that fucking dog would blow my brains out, but I'm just trying to make sure everything's fine. When should I first check, if inoculating happened this last Sunday night?


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



Edited by Circle K (09/28/13 06:18 PM)


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Invisibletbagtag
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855163 - 09/17/13 07:43 PM (10 years, 4 months ago)

Man, with that killer setup you should be ready to fruit in days.


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Invisiblemaddchef
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Re: WBS Jars inoculated, now for the meantime.. [Re: tbagtag]
    #18855185 - 09/17/13 07:48 PM (10 years, 4 months ago)

Lol the shit is deep in here tbag


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibletbagtag
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Re: WBS Jars inoculated, now for the meantime.. [Re: maddchef]
    #18855192 - 09/17/13 07:50 PM (10 years, 4 months ago)

Did OP spawn already?  I missed that part.


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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855196 - 09/17/13 07:51 PM (10 years, 4 months ago)

Quote:

Circle K said:
without a PC, I just boiled 4 jars at a time for 90 mins




You'll be getting ounces for sure.

Quote:

We have a small space heater pointed at the box on one end, with a heat lamp overlooking it. The box gets pretty warm, but the jars seem fine.




I know that cubes prefer to colonize in the mid 70s but 90+ should work fine too, I bet they'll go twice as fast.



Edited by Trusted cuItivator (09/17/13 07:54 PM)


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OfflinePrinceShroom
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18855224 - 09/17/13 07:59 PM (10 years, 4 months ago)

Im glad you did your research cause this is an epic grow.  I totally understand why you arent posting pics.
:failboat:

You should use that handgun
:suicide:


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: tbagtag]
    #18855330 - 09/17/13 08:22 PM (10 years, 4 months ago)

I forgot to mention that the endpoint is spawning these into 5050 Monotubs. I've done the research, but I have a game plan for even if one or two come out with no contams. At least one is gonna sit around and become a grain master, and I've got smaller containers for if I only get a few pint jars' worth.

I know that on this site there's really nothing to worry about, but I just want to keep my head down. There's no definite for how many shroomerites get busted out of the whole, but I'm certainly not risking my ass. Not to mention that dog likes watching me use my computer, and while he can read really well, this time I'm just watching him lick his own asshole.

I can give you guys pictures of the dog, at least, just not with his coke or his handgun. That would be illegal. :frown:

Quote:

tbagtag said:
Did OP spawn already?  I missed that part.




Yeah, I've inoculated the jars, and they're sitting in my closet now. While they're innoculating, how much should I worry about FAE? The scientific part of my brain wants to cut off all airflow to the box and let the birdseed get colonized on its own, but I can't be sure, so I didn't tape the box off. I Lysol the FUCK out of that closet a handful of times a day, just to be sure. Blows back into the room and makes it smell like a hospital.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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OfflinePrinceShroom
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855372 - 09/17/13 08:30 PM (10 years, 4 months ago)

If you did your research you missed the part where you cant boil grain jars, only cakes.
Steam sterilization will not work for grain.


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: PrinceShroom]
    #18855486 - 09/17/13 09:00 PM (10 years, 4 months ago)

Quote:

PrinceShroom said:
If you did your research you missed the part where you cant boil grain jars, only cakes.
Steam sterilization will not work for grain.




Yeah, I figured that. I did enough research and read all about endospores, but I've read a few posts that tell me that while there is still a high contam chance, there is still possibility for successful fruiting, and to get to my next step, a couple/few jars is really all I need. If/When the jars contaminate, I'll just resterilize them and wait until my financial situation allows me to buy a pressure cooker. There's a 22 quart one up on ebay for about 110 bucks right now, I'm eyeing up that one.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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Invisiblemaddchef
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855619 - 09/17/13 09:32 PM (10 years, 4 months ago)

Without being a total Dick here......do some more research and keep it so simple you think it ain't work, seriously.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18855991 - 09/17/13 10:58 PM (10 years, 4 months ago)

I DID do my research, you dunces. I've read a few threads where people boiling WBS have had success, so I'm understanding that while the majority of my jars may come out contaminated, I'm still working with the tools at hand to start producing for myself before I get a PC. It's funny that this is the second or third time I've had to bring this up.

I have trouble reading sarcasm through the internet, but thanks to whoever's genuinely advising me, here. My heater got the Rubbermaid box pretty warm, but definitely not 100. It's Tuesday night where I'm at, and figuring I innoc'd on Sunday, I haven't seen any growth, at all. I shook a few jars (Can I do that?) In my incubator, and there's nothing I can see. Again, I've got 6 Hillbilly, and 6 Tasmanian. I like the both so much that I'm gonna try half and half in a few tubs later on down the line, to see if there are any good results.

So let's bear in mind that I know it was a fuck-up to not PC my jars. Whoopee, unless you guys want to take your dickery further and just keep poking at it.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisibleSteyner
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18856649 - 09/18/13 03:12 AM (10 years, 4 months ago)

Too hot will just encourage bacterial growth dude. You want high 70s, 80 max. Which is room temperature in most parts. And you dont need the dark. these babies need gas exchange so don't tape up you box and bags. If you are using the bags to hide them fine, but really you just want them out on the shelf. And no more need for lysol man, they got the polyfil so no germs will get in from outside.


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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Steyner]
    #18857450 - 09/18/13 11:04 AM (10 years, 4 months ago)

I'm only staying for the lulz. There's no hope nor suggestions that will be of help or even listened to for that matter. Between the incubator, lysol, lack of PC, and the 10 other things that CircleK has actually said he did that will fail his grow I personally can't wait.

Where are those dog pictures?


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18859967 - 09/18/13 07:39 PM (10 years, 4 months ago)

Quote:

Steyner said:
Too hot will just encourage bacterial growth dude. You want high 70s, 80 max. Which is room temperature in most parts. And you dont need the dark. these babies need gas exchange so don't tape up you box and bags. If you are using the bags to hide them fine, but really you just want them out on the shelf. And no more need for lysol man, they got the polyfil so no germs will get in from outside.




For being one of the first people to actually make a suggestion, thanks.

I moved them to just being out in my upper closet, with a HEPA running on low, through it. I just had it figured they wouldn't need light nor gas, due to the mycelium needing to spread, but I'll take your word on this. Removed the heater and the lamp. All it really took was someone to tell me what I need to change around, you guys. The last part of Doc's tek said to incubate at 80, so that's what I figured. I tested a jar with a thermometer and had the ballpark of the mid-eighties with the heater and lamp on them (We fine tuned the fuck out of that setup), but if it's gonna fuck up my colonizing, fixing it up is easy.

When my jars colonize, I'll let you guys know.


Quote:

bodhisatta said:
I'm only staying for the lulz. There's no hope nor suggestions that will be of help or even listened to for that matter. Between the incubator, lysol, lack of PC, and the 10 other things that CircleK has actually said he did that will fail his grow I personally can't wait.




So, does most of this board just try to act like smart-asses? Does it make your E-peen feel any bigger? I never said I wasn't gonna change shit around, on account of someone just having made a suggestion, rather than some smart-assed comment.

I'm really trying to do this here, you guys. I'm taking every suggestion I can get. I'm trying to be respectful and if I didn't need help I wouldn't come here, but you guys just seem to be dishing out arrogance. It's not that I can't take it, that's no problem, it's that I'm genuinely trying to get shit done.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisibleSteyner
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18864037 - 09/19/13 05:27 PM (10 years, 4 months ago)

No sweat man. These guys are just giving you a hard time because there is a general concensus of how grains should be done for maximum effeciency, and although you mentioned docs tek you seem to be following none of the rules. :-) anyway i sincerely hope at least one of the dozen make it so you can move on to 1. First make a grain liquid culture to ensure 10 more syringes if that is an option for you then 2. Once recovered transfer to properly sterilized and hydrated grain jars to get to unlimited mycelium for life.

Its been four days now rite, any small white spots on those grains yet? Should be close if not there yet. Although can take up to three weeks depending on the quality of your ms syringes. Expect the jars you shook to to take longer than the others, shaking before you see any strong growth is strongly advised against. You want to shake once you got nice strong growth, size of a golfball maybe. How is the moisture content looking in those babies? If you soaked and dried well they should look damp, not wet. Dont worry about condensation if you have those at the tops, at the heat you had them on i suspect you have a lot. However its just a nuisance, not the end of the world. At normal temps like you have now it should dissappear in a day or so.

Next time flame sterilize the needle before and between each jar. Sure you can get a lighter or a fking match for that matter? And dont try to resterilize jars that have contamed man, chuck the grain and restart. Try having a bigger size poly filled hole per jar too, or if only nails available, make three or four holes in each jar. Will allow for better gas exchange and faster growth. Just dont fuck with the inoculated jars now, have patience and let me know how it turned out!


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Steyner]
    #18867381 - 09/20/13 12:03 PM (10 years, 4 months ago)

Quote:

Steyner said:
No sweat man. These guys are just giving you a hard time because there is a general concensus of how grains should be done for maximum effeciency, and although you mentioned docs tek you seem to be following none of the rules. :-) anyway i sincerely hope at least one of the dozen make it so you can move on to 1. First make a grain liquid culture to ensure 10 more syringes if that is an option for you then 2. Once recovered transfer to properly sterilized and hydrated grain jars to get to unlimited mycelium for life.

Its been four days now rite, any small white spots on those grains yet? Should be close if not there yet. Although can take up to three weeks depending on the quality of your ms syringes. Expect the jars you shook to to take longer than the others, shaking before you see any strong growth is strongly advised against. You want to shake once you got nice strong growth, size of a golfball maybe. How is the moisture content looking in those babies? If you soaked and dried well they should look damp, not wet. Dont worry about condensation if you have those at the tops, at the heat you had them on i suspect you have a lot. However its just a nuisance, not the end of the world. At normal temps like you have now it should dissappear in a day or so.

Next time flame sterilize the needle before and between each jar. Sure you can get a lighter or a fking match for that matter? And dont try to resterilize jars that have contamed man, chuck the grain and restart. Try having a bigger size poly filled hole per jar too, or if only nails available, make three or four holes in each jar. Will allow for better gas exchange and faster growth. Just dont fuck with the inoculated jars now, have patience and let me know how it turned out!




I appreciate all that. I tried to follow it as best as I can, but I know I put a lot of my own spins on it. More than one jar has these beautiful little white spots, and they certainly don't look like trich, so that's a total plus. I will have to look up liquid cultures and how to make them; I've heard the term, but I haven't looked into it much. I had grain masters in my plans, but that sounds a ton more efficient. I know it's a push, but do you know if I would have any luck transferring the birdseed to a more forgiving substrate? Yield doesn't matetr so much to me, as much as making sure I don't have to worry about contamination.

It's been four days, yeah. I inoculated a few jars on Saturday, but those aren't showing anything. I've got spots on more than one jar, and I'm gonna leave those aside. I shook one before reading this, but that was only because I was searching for growth on the inside. As soon as that myc gets nice and fat, we'll facilitate redistribution. I'm actually pretty excited for this! I'm a total noob I know, but getting those spots tells me that I've done right enough.

When I reinnoculated my jars I did it near the stove, and got those syringes red hot. I wanted to make sure the only thing I did wrong that time was boiling - Each jar has a 1/4th inch hole stuffed with tyvek, with two coffee filters rubber banded to the top. We got the holes that wide by being nice and resourceful, (Getting really stoned and using screws to get some girth up in that bitch), but now we're just gonna let them sit for a few days. Seeing these white spots, I know that there's nothing I'll need to add to these jars to keep growth up, and I'm a bit intimidated that anything I try to fix on them will contaminate them.

I really want to upload some pictures you guys, but you have to understand the whole paranoia thing, about putting pictures of that on the internet, and shit. I know tens of dozens of people post them on a daily basis, but is it dangerous here, if at all?


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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OfflinePrinceShroom
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18868288 - 09/20/13 03:42 PM (10 years, 4 months ago)

Id say shroomery is one of the safest places to post TBH.  They strip all EXIF data fr all pics.


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18885250 - 09/24/13 02:40 PM (10 years, 4 months ago)

Hey guys, checking back in.

An EXIF scrubber would be just what I need. I use an older digital camera that probably doesn't even have data like that, but my general interest is covering every base.

Anyway, I'm uploading these couple pictures of my best jars. I'm picking up some verm and coir later on, and when I'm able to, I'm gonna grab some BRF and a couple dozen more jars. The PC comes later on, probably next month, on account of me being done with my hefty bag of dried WBS for the meantime!

Anyway, these jars look great, could someone give me a rule of thumb on shaking them? One's got a bottom that's awesome, and going off the golf ball rule, I'd say at least one's ready. I'm most likely gonna shake it when I get home tonight, to give it that little extra edge.

One of the jars has an icky dark-brown, well, I don't know what it is at the bottom, I'll get a picture later on. Our first jar, colonizing way before the others, developed a nasty clump of mold, but that's what we've been ready for, having not PC'd grains, although I'm still blown away that these jars are doing so good!





--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18886136 - 09/24/13 06:22 PM (10 years, 4 months ago)

I'm no expert but all of those jars look bad maybe 1 of them is good. The coffee filters don't help anything.


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18886322 - 09/24/13 07:18 PM (10 years, 4 months ago)

Quote:

bodhisatta said:
I'm no expert but all of those jars look bad maybe 1 of them is good. The coffee filters don't help anything.




What do you mean? After I noc'd them up I shook each jar, which would explain the erratic growing. My camera may have not gotten them enough, but smell-wise, we're not getting anything nasty off of any of them.

I know I'm probably asking some elementary questions, here, but would it be ill-advised to g2g any one of these jars to a different substrate? I'm making the switch to PF-Style jars and I want to make sure they actually colonize.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18886342 - 09/24/13 07:23 PM (10 years, 4 months ago)

Idk how you would g2g to a PF jar unless it didn't have a verm barrier and then it would just be a brf cake in a half pint with a grain lid. Some of them look bacterial but like I said I'm not expert and have only seen a couple dozen of my own grain jars but none have ever looked like that.


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18886360 - 09/24/13 07:26 PM (10 years, 4 months ago)

What kind of grain are you using, if you don't mind me asking? And what strains are you using?


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18886377 - 09/24/13 07:30 PM (10 years, 4 months ago)

Strain doesn't matter in the slightest the strain is P.Cubenis but to not be a dick I've used GT B+ Creeper AA+ and they all act exactly the same with MS inoculations aside from AA+ maybe being a little bit slow on average to colonize.

http://www.shroomery.org/forums/showflat.php/Number/18836421#18836421
There's a lot of links in that post about strain discussion by RR and other TC's

I always use organic rye.

On second look up close the top middle and bottom right are the only ones that look suspect the others could all be ok for all I know but I've never seen growth look quite like that on grains but you did shake them right away too.


Edited by Trusted cuItivator (09/24/13 07:35 PM)


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18886520 - 09/24/13 08:09 PM (10 years, 4 months ago)

Quote:

bodhisatta said:
Strain doesn't matter in the slightest the strain is P.Cubenis but to not be a dick I've used GT B+ Creeper AA+ and they all act exactly the same with MS inoculations aside from AA+ maybe being a little bit slow on average to colonize.

http://www.shroomery.org/forums/showflat.php/Number/18836421#18836421
There's a lot of links in that post about strain discussion by RR and other TC's

I always use organic rye.

On second look up close the top middle and bottom right are the only ones that look suspect the others could all be ok for all I know but I've never seen growth look quite like that on grains but you did shake them right away too.




Thanks, jefe. I cross posted my pics over in the contam forum. The one red light is one jar (Not pictured) Had a white blob half the size of a thumbprint, above the substrate, growing off of a single seed stuck to a side, which I really can't be sure about.

I appreciate the help, really. As soon as I've got colonization, I'm planning on spawning around half to some trays I picked up at the good ol' mercado. I lost track of my Taz and Hillbilly strains in whichever jar, so that's definitely going to be interesting, when they begin to fruit!


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18886550 - 09/24/13 08:16 PM (10 years, 4 months ago)

Quote:

Thanks, jefe. I cross posted my pics over in the contam forum. The one red light is one jar (Not pictured) Had a white blob half the size of a thumbprint, above the substrate, growing off of a single seed stuck to a side, which I really can't be sure about.




That always happened to me the first time I made grain jars and was to impatient and shook them more than the one time at 30% and then again at 100% prior to spawning. Always having to make my own mistakes to learn why people say the things they do for a reason.


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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18886684 - 09/24/13 08:48 PM (10 years, 4 months ago)

Look good to me, but I am usually wrong anyway so that doesn't mean much


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Re: WBS Jars inoculated, now for the meantime.. [Re: Sockadin]
    #18887457 - 09/25/13 12:10 AM (10 years, 4 months ago)

Thanks, helps out a little.

I shook each jar around 30 mins back, so now it's pretty much a waiting game. Some clumps were pretty tough to bash off the walls, but I gave each a really good shake. It seems I filled a few of them a little too much, which made shaking kind of a hassle, but instead of pouring a bit out, I kept it safe and shook with each jar closed, filters still on.

I'm gonna check on them periodically, and hopefully sometime this weekend I'll see some colonization, and I'll be sure to snap a few pics for you guys. I'm picking some more supplies up pretty soon, and I'm thinking about using the last cc of each of my two syringes to PF tek-style noc up two extra jars with it, and I'll have those isolated from the rest. Here's hoping this starts looking good!


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18887756 - 09/25/13 03:15 AM (10 years, 4 months ago)

they look great, congrats man. in two days' time they will look near fully colonized, but give it another week. use the best one (make sure no contams and colonized 100%) to make grain liquid culture!

what you do is boil some water, and draw it into an empty syringe with needle whilst boiling. empty the syringe wherever just not back in the pot, and repeat about 4 or 5 times. on the last draw do not empty the syringe, but place the cap on (the cap should be in the boiling water as well to sterilize) and put it in the fridge to cool down. Let it cool properly.

now for the fun part. you want to shake your jar, then flame sterilize the syringe needle and inject the now sterilized and cooled water into the jar through the polyfill/tyvek. keep the syringe in the jar and give it a nice shake to get the loose mycelium into the water. then suck out as much water as possible, remove the syringe and tape up the hole you just made if through the tyvek. Voila, mycelium laden water in your syringe, as good as, no even better than a spore syringe. you won't fill it all the way as a lot of the water will be sucked up by the grains but don't worry, a little goes a long way.

you can use this syringe to noc a jar of sterilized water with one of your special lids and a piece of broken glass/shotglass inside. the myc should grow within a week and the solution will be super clear as you don't need any sugars in it since the water picked up nutrients when shaken through the grains. so contams are easy to spot. from here you can get as many syringes as you need, full of active already germinated and already growing mycelium! the glass in the jar is used to break up the clouds of mycelium to make it easier to suck into your syringes whenever you need to draw from it.

use what's left of the syringe in more grain jars, hopefully by then you have the pc to sterilize things properly! if the syringe is left out for about a week the myc water may get very thick, and at some point maybe after 3 to 4 weeks it will clog the needle so before that, you want to move it to the fridge where it will last for months. same with the liquid culture jar once you see it's colonized far enough.

looking forward to see your pic of that beautiful clear jar with white clouds of mycelium growing inside!


Edited by Steyner (09/25/13 03:43 PM)


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Re: WBS Jars inoculated, now for the meantime.. [Re: Steyner]
    #18889238 - 09/25/13 01:11 PM (10 years, 4 months ago)

Thanks! I was planning on making liquid cultures with the remaining 1cc in each of my syringes, but the idea for getting myc straight from the jars never occurred to me!

Some of the myc is actually poking through to the face of my jars, and I noc'd them up at around 10 last night. It's nothing big, I'll snap some pics if I get the chance (Not at my pad now), but I'd measure it as a fifth of a centimeter-sized growths that are poking through. I'm hoping they'll look good after a few days!

I went out this morning and stocked up on cleaning supplies, and picked up a 16oz bottle of clear Karo with Vanilla, and I'm boiling up some LC as I type this. I'm gonna let it cool throughout the day, and use up my jars tonight to 'noc up each jar. As soon as those go good, I'm gonna start transferring them to more and more jars.


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18889515 - 09/25/13 02:08 PM (10 years, 4 months ago)

There's a good thread about the dangers of LC by FrankHarrigan. Grain to grain transfers can expand your spawn supply by  10. 1 agar dish becomes 4 quarts, which can becoe 40 quarts.


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18889694 - 09/25/13 02:59 PM (10 years, 4 months ago)

Quote:

Circle K said:The scientific part of my brain




This was when I knew this had to be trolling as I knew dog would have removed this with a .44 by now :lol:


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Re: WBS Jars inoculated, now for the meantime.. [Re: Skinty]
    #18896841 - 09/27/13 12:13 AM (10 years, 4 months ago)

Quote:

Skinty said:
Quote:

Circle K said:The scientific part of my brain




This was when I knew this had to be trolling as I knew dog would have removed this with a .44 by now :lol:




He actually tried, the other week. We convinced him his gun didn't have any bullets in it, so he shot the TV instead.

Anyway, the jars are really coming along. I'm gonna give them another solid week or two, but the mycelium's just shot out, now that I've shaken them! My liquid culture jars are displaying tiny, tiny little spore/myc particles when I swill the jars, so it's gonna be neat seeing those strand together in a few days!

The awesome thing about liquid culture, I've been reading, is that so long as you either have spores or a healthy substrate, you can always use that method, even without a pressure cooker, to conserve spores in that fashion. It could be that I'm all kinds of stoned now, but the ability to even get healthy myc off of a dried mushroom is just all kinds of awesome.


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18903854 - 09/28/13 06:17 PM (10 years, 4 months ago)

My jars have taken off, for sure. I've got a good amount of pics of the mycelium in them, along with my two liquid culture jars. There's pretty much nothing but a few near-microscopic bits swirling about in the jars, but figuring I noc'd them each up with just 1CC, I figure it'll take a little while to notice any activity.

Anyway, onto the pictures I've snapped. I'm double sure and run mine through an EXIF scanner just to be safe, but most of them look great.

Here's the lineup. You can easily tell which one is doing the best, but it's got me a bit worried.


I've got no clue what this is, and I'm hoping it's something benign, can anyone diagnose it?


Sans that brown shit, these three best jars are my pride and joy of this entire process.


I snapped a few more, each of a different jar.





I'm gonna include pics of my liquid cultures, each in a pint jar with 1 1/2 tsp of Karo light;



I shake my jars once or twice a day to encourage growth, should I be shaking them more/less? I read that you can make a pint of LC in two days with a magnetic stirrer, but I didn't stick an agitator in either jar. Should I give that a shot, at some point?

Let me know how I'm doing, you guys, this is starting to become awesome. I'm gonna make a few more syringes off of the jars before I put them into trays/tubs (We don't need cakes, where we're going.).

I feel like this was a really close save, if at least half of what I have now goes right. Whatever keeps me from needing to buy more spores tells me that I've done something right.


--------------------
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18904456 - 09/28/13 08:41 PM (10 years, 4 months ago)

Wat does dog think :shrug:


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Re: WBS Jars inoculated, now for the meantime.. [Re: Skinty]
    #18904604 - 09/28/13 09:18 PM (10 years, 4 months ago)

Honestly I wouldn't make any Grain LCs off of a non PCed jar. The odds just aren't in your favor. Shake less. Id shake maybe 2 times once at 30% colonization and once at maybe 60%. Shaking too much actually damages them and slows growth


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Re: WBS Jars inoculated, now for the meantime.. [Re: Chaos Creations]
    #18904837 - 09/28/13 10:17 PM (10 years, 4 months ago)

Quote:

Skinty said:
Wat does dog think :shrug:




You wouldn't believe it. Every question we ask him, he answers "Yes". That's it. He's either doing that or yelling at us to "Make the shrooms" already. He tried to send me to get him some more bullets, but I slashed my own tires.

Quote:

Chaos Creations said:
Honestly I wouldn't make any Grain LCs off of a non PCed jar. The odds just aren't in your favor. Shake less. Id shake maybe 2 times once at 30% colonization and once at maybe 60%. Shaking too much actually damages them and slows growth




Alright, I'll hold off of that, and work with the next batch I'm able to PC. I've shaken after inoculation, then when my jars were at
20%. Now that they've really taken off, I'm thinking I'll shake some of the lesser performers, that are still quite performing, hah.


--------------------
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18904876 - 09/28/13 10:27 PM (10 years, 4 months ago)

Those are all screwed
You did almost everything right except the pressure cooker part.
I went to goodwill and found a pressure cooker for like $9. Then ordered the 15psi weight for the lid online for like $6.
Do everything you did again, except do it right, with a pressure cooker. And do some more reading.
I would just use those jars for an outdoor patch. And redo everything the right way with a PC, then G2G, then spawn to bulk/make LC, agar, etc.


Edited by Pirax (09/28/13 10:30 PM)


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Re: WBS Jars inoculated, now for the meantime.. [Re: Pirax]
    #18904881 - 09/28/13 10:28 PM (10 years, 4 months ago)

Quote:

Pirax said:
Those are all screwed
You did almost everything right except the pressure cooker part.
I went to goodwill and found a pressure cooker for like $9. Then ordered the 15psi weight for the lid online for like $6.
Do everything you did again, except do it right, with a pressure cooker. And do some more reading.





Damn, why am I getting all the downers? I'd say things have started looking really good.


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18904888 - 09/28/13 10:32 PM (10 years, 4 months ago)

All of those jars are obviously contaminated. You can visibly see bacteria growing, and possibly mold.
Read the edit I made on my original post, you might be able to make an outdoor patch with those but I would not attempt to fruit those inside your house.


Edited by Pirax (09/28/13 10:32 PM)


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18909095 - 09/29/13 09:24 PM (10 years, 3 months ago)

Quote:

Circle K said:
Quote:

Skinty said:
Wat does dog think :shrug:




You wouldn't believe it. Every question we ask him, he answers "Yes". That's it. He's either doing that or yelling at us to "Make the shrooms" already. He tried to send me to get him some more bullets, but I slashed my own tires.

Quote:

Chaos Creations said:
Honestly I wouldn't make any Grain LCs off of a non PCed jar. The odds just aren't in your favor. Shake less. Id shake maybe 2 times once at 30% colonization and once at maybe 60%. Shaking too much actually damages them and slows growth




Alright, I'll hold off of that, and work with the next batch I'm able to PC. I've shaken after inoculation, then when my jars were at
20%. Now that they've really taken off, I'm thinking I'll shake some of the lesser performers, that are still quite performing, hah.





Don't shake after inoculation. Tell your dog to stop humping the jars there is a little red rocket mark on one of them.


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Re: WBS Jars inoculated, now for the meantime.. [Re: Sockadin]
    #18909192 - 09/29/13 09:48 PM (10 years, 3 months ago)

I shot dog.


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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18909248 - 09/29/13 10:00 PM (10 years, 3 months ago)

Quote:

bodhisatta said:
I shot dog.




You must not have hit him.

He melted down my grandma's holocaust ring and retrieved a spent casing. He's asking us for gunpowder, really nicely. He's so cute too, I almost want to go and pick him up some. Honestly, this little guy needs us to go pick up everything, but we seriously have no idea how he gets all of this heroin.

Does anyone know if dogs can actually OD on heroin, if at all? He just keeps doing it, like, over and over. I took this picture of him while he was all frothed over and just staring out into space, I hope he doesn't come to and remember it.


HOLY SHIT, THIS IS A HUGE PICTURE


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18913190 - 09/30/13 09:04 PM (10 years, 3 months ago)

Hey guys, I don't get paid until late October, but I wanted to do everything I can with these jars. If there's anything cheap I can pick up (Around like, five bucks cheap) I can pick it up, but my dog took my money and tried to spend it on heroin again.

I've got two small bales of straw, and a 24oz bag of Bob's Red Mill BRF. Does anyone know if I can use these to kick off a few trays, for these WBS jars that are finally coming to be mostly white? I was gonna pick up some seed-starting Jiffy mix, but I want to get your guys' input first. I found a few straw teks that I could follow to the T, no sweat.


Edited by Circle K (09/30/13 09:05 PM)


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18913342 - 09/30/13 09:36 PM (10 years, 3 months ago)

Yes you can inoculate 24lbs of straw with 3 lbs of BRF and 1/16 oz of black tar heroin. I don't know what the results would be but I am sure it would be awesome!


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Re: WBS Jars inoculated, now for the meantime.. [Re: Sockadin]
    #18914047 - 10/01/13 12:23 AM (10 years, 3 months ago)

Quote:

Sockadin said:
Yes you can inoculate 24lbs of straw with 3 lbs of BRF and 1/16 oz of black tar heroin. I don't know what the results would be but I am sure it would be awesome!





What the fuck?


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18920837 - 10/02/13 12:28 PM (10 years, 3 months ago)

We're gonna birth out cakes this Saturday, into a few trays we'll cut holes into, for after colonization. We'll crumble 'em up into a mix of verm, coir, and coffee, going off of large_dose's easy coir tek. Gonna be mobbing the Starbucks near me for their ground coffee, although from what I've read, I'm hoping I don't get stuck with 49 more pounds than I need! Either way, our jars look great. Referring to bulk jar pics, they're already ready to fruit, but I want to let them consolidate a little bit more. If I'm spawning to bulk, would it not be smart to wait until I see pins, in the jar?

Again, I've got that Aspen Bedding, which is pretty much shredded woodchips, and a bag of BRF. I pick up my verm and coir this Saturday, and I was wondering if that would be a viable mix. I would be mixing a ratio'd amount of BRF in to accomodate for the fact that P. Cubensis do NOT absorb nutrients from wood.

Does that sound viable? If it is, I've got a jar I could put straight into fruiting tomorrow by the soonest, after pasteurizing my substrates I have now. It would be just a small tray out of a pint jar, so it'd hardly even be called an experiment.


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18920881 - 10/02/13 12:37 PM (10 years, 3 months ago)

Quote:

Circle K said:
We're gonna birth out cakes this Saturday, into a few trays we'll cut holes into, for after colonization. We'll crumble 'em up into a mix of verm, coir, and coffee, going off of large_dose's easy coir tek. Gonna be mobbing the Starbucks near me for their ground coffee, although from what I've read, I'm hoping I don't get stuck with 49 more pounds than I need! Either way, our jars look great. Referring to bulk jar pics, they're already ready to fruit, but I want to let them consolidate a little bit more. If I'm spawning to bulk, would it not be smart to wait until I see pins, in the jar?



Leave out the coffee unless you plan on properly pasteurizing the substrate material.

If spawning to bulk, you do not want the spawn to consolidate at all.

You want to spawn right at 100%, while the mycellium is still "young and vigorous".

Spawning with pins present is ok, but is in no way needed.

The pins will turn back into mycellium.


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Re: WBS Jars inoculated, now for the meantime.. [Re: PussyFart]
    #18922502 - 10/02/13 05:16 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:

Leave out the coffee unless you plan on properly pasteurizing the substrate material.

If spawning to bulk, you do not want the spawn to consolidate at all.

You want to spawn right at 100%, while the mycellium is still "young and vigorous".

Spawning with pins present is ok, but is in no way needed.

The pins will turn back into mycellium.




Thanks, NaH! I figure that the best performing jar I have (Check the pics) is either gonna start pinning, or that nasty bacteria at the bottom will do something to it (That shit looks like it's spreading, eww.). Would my 60/40 Animal Bedding (Wood shreds) and BRF idea do anything, if I mixed it in with that best jar? I've seen success on only woodchips, but I know Cubensis aren't woodlovers, so I wanted to supplement with the BRF. Do you know if it would be worth it, even as a test?

EDIT: It seems like an 80/20 mix would be a lot better, actually, after seeing a post RR made. They're just wood shavings I picked up from a dollar store, so I figure I shouldn't have to soak them as long, but I'll definitely simmer them a bit, before mixing them into the main substrate.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



Edited by Circle K (10/02/13 05:25 PM)


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #19012235 - 10/22/13 05:30 AM (10 years, 3 months ago)

Curious, did you ever get a fruit from those contaminated jars? Probably not i guess.


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Re: WBS Jars inoculated, now for the meantime.. [Re: Steyner]
    #19024921 - 10/24/13 10:48 AM (10 years, 3 months ago)

Quote:

Steyner said:
Curious, did you ever get a fruit from those contaminated jars? Probably not i guess.




I've got a tray with twenty pins, actually!

I fruited two trays, one did just that, the other hasn't shown much other than full colonization, and some condensation. It smells a little sweet in there, but it looks fine. I'm gonna snap a few pictures.

I shook a few jars which revealed contam'd insides, so I tossed them. My grain jar that looked the best was used as a g2g host for seven BRF jars. As silly as that sounds, they're all sitting at 100% right now, and I'm letting them consolidate before I birth them. I'm gonna crumble 'em into a 5050 mix (Still doing my homework on that part), and I'm planning on loading them into some shoebox trays (6QT I believe).

It's a longshot, but I made a syringe with my jar, the one used for the other verm/brf jars. The science here is that if none of them contam, the syringe should be fine. It looks alright as well; I keep it in the fridge, and through the tube, I can see little cords of myc throughout!

For now, my eyes are just on this tray. It's got an alright pinset on it, nothing to write home about, but the fact that I've come this far is awesome for me. I'm working around not having a PC, but I'm getting that fixed pretty soon. I'm gonna dig around for my camera and show you all the glory, hah.

EDIT: Here's some pics.



I'm so proud of these little guys. The caps are pushing up against the lid, which is putting the pressure on me to make a bigger FC where I can leave the lids off.


That earthy, mushroomy smell comes off of this one, but there's an air of sweetness to it.

I'm due to birth nine trays' worth, and I'm considering tossing that last one. The smell just isn't a good sign.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



Edited by Circle K (10/24/13 11:08 AM)


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InvisibleSkinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #19046585 - 10/28/13 03:23 PM (10 years, 3 months ago)

Quote:

Circle K said:
Quote:

Steyner said:
Curious, did you ever get a fruit from those contaminated jars? Probably not i guess.




I've got a tray with twenty pins, actually!

I fruited two trays, one did just that, the other hasn't shown much other than full colonization, and some condensation. It smells a little sweet in there, but it looks fine. I'm gonna snap a few pictures.

I shook a few jars which revealed contam'd insides, so I tossed them. My grain jar that looked the best was used as a g2g host for seven BRF jars. As silly as that sounds, they're all sitting at 100% right now, and I'm letting them consolidate before I birth them. I'm gonna crumble 'em into a 5050 mix (Still doing my homework on that part), and I'm planning on loading them into some shoebox trays (6QT I believe).

It's a longshot, but I made a syringe with my jar, the one used for the other verm/brf jars. The science here is that if none of them contam, the syringe should be fine. It looks alright as well; I keep it in the fridge, and through the tube, I can see little cords of myc throughout!

For now, my eyes are just on this tray. It's got an alright pinset on it, nothing to write home about, but the fact that I've come this far is awesome for me. I'm working around not having a PC, but I'm getting that fixed pretty soon. I'm gonna dig around for my camera and show you all the glory, hah.

EDIT: Here's some pics.



I'm so proud of these little guys. The caps are pushing up against the lid, which is putting the pressure on me to make a bigger FC where I can leave the lids off.


That earthy, mushroomy smell comes off of this one, but there's an air of sweetness to it.

I'm due to birth nine trays' worth, and I'm considering tossing that last one. The smell just isn't a good sign.




Why did you use whipped cream as a bulk material?


--------------------


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OfflineKiya_Star427
Live FREE or be a SLAVE!


Registered: 10/14/13
Posts: 355
Loc: New York Flag
Last seen: 5 years, 1 month
Re: WBS Jars inoculated, now for the meantime.. [Re: Skinty]
    #19046750 - 10/28/13 03:48 PM (10 years, 3 months ago)

Quote:

Skinty said:
Quote:

Circle K said:
Quote:

Steyner said:
Curious, did you ever get a fruit from those contaminated jars? Probably not i guess.




I've got a tray with twenty pins, actually!

I fruited two trays, one did just that, the other hasn't shown much other than full colonization, and some condensation. It smells a little sweet in there, but it looks fine. I'm gonna snap a few pictures.

I shook a few jars which revealed contam'd insides, so I tossed them. My grain jar that looked the best was used as a g2g host for seven BRF jars. As silly as that sounds, they're all sitting at 100% right now, and I'm letting them consolidate before I birth them. I'm gonna crumble 'em into a 5050 mix (Still doing my homework on that part), and I'm planning on loading them into some shoebox trays (6QT I believe).

It's a longshot, but I made a syringe with my jar, the one used for the other verm/brf jars. The science here is that if none of them contam, the syringe should be fine. It looks alright as well; I keep it in the fridge, and through the tube, I can see little cords of myc throughout!

For now, my eyes are just on this tray. It's got an alright pinset on it, nothing to write home about, but the fact that I've come this far is awesome for me. I'm working around not having a PC, but I'm getting that fixed pretty soon. I'm gonna dig around for my camera and show you all the glory, hah.

EDIT: Here's some pics.



I'm so proud of these little guys. The caps are pushing up against the lid, which is putting the pressure on me to make a bigger FC where I can leave the lids off.


That earthy, mushroomy smell comes off of this one, but there's an air of sweetness to it.

I'm due to birth nine trays' worth, and I'm considering tossing that last one. The smell just isn't a good sign.




Why did you use whipped cream as a bulk material?




that shit looks whiter then marshmallows, damn... LOL it just makes you smile!!


--------------------


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