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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Why did my LC not work?
    #18852992 - 09/17/13 10:21 AM (10 years, 4 months ago)

I made an LC with Mesquite honey (extra light amber grade a honey) in a mason jar 2 weeks ago, injected a ton of pan cyan sperms and all I have are bigger looking spores and a few mycelium strands floating in the juice. Did this in a glovebox and kept things as sanitary as I could. any ideas why im having these results?


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InvisibleOeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon Flag
Re: Why did my LC not work? [Re: TheLoopIs]
    #18853019 - 09/17/13 10:27 AM (10 years, 4 months ago)

At any rate, chances are, your spores aren't clean enough to be multiplied in a nutrient solution without contam issues running wild. Pan spores can be harder to germinate sometimes.
What's your ratio? Too rich of a sugar solution and nothing ideal will grow


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
Re: Why did my LC not work? [Re: Oeric McKenna]
    #18853056 - 09/17/13 10:36 AM (10 years, 4 months ago)

When making an LC you shouldn't be using a "ton" of spores anyways.  You should be using as few spores as possible in almost every situation.


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Why did my LC not work? [Re: Mush4Brains]
    #18853391 - 09/17/13 12:36 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
When making an LC you shouldn't be using a "ton" of spores anyways.  You should be using as few spores as possible in almost every situation.




Exactly this. That could be most of your problem, aside from the fact that you probably do have at least some contams in  syringe that are now in the most ideal environment you can give them.


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: Why did my LC not work? [Re: bodhisatta]
    #18853417 - 09/17/13 12:47 PM (10 years, 4 months ago)

Fuck LCs....Learn agar and G2G transfers....


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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Re: Why did my LC not work? [Re: PussyFart]
    #18854474 - 09/17/13 05:07 PM (10 years, 4 months ago)

lol notahacker. Do u have any easy tek's to suggest for agar?
thank u everyone for the help!


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InvisibleSpitballJedi
Ancient Astronaut
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Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
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Re: Why did my LC not work? [Re: PussyFart]
    #18854514 - 09/17/13 05:19 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
Fuck LCs....Learn agar and G2G transfers....



:solidnod:


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
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Re: Why did my LC not work? [Re: TheLoopIs]
    #18854622 - 09/17/13 05:43 PM (10 years, 4 months ago)



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Offlinemastercultivator
Master Cultivator
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Registered: 08/27/13
Posts: 653
Last seen: 7 months, 2 days
Re: Why did my LC not work? [Re: TheLoopIs]
    #18854638 - 09/17/13 05:48 PM (10 years, 4 months ago)

I need more information to make any statement.

Thank you.

-MC


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