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mrbeanstalk
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Registered: 03/03/13
Posts: 81
Last seen: 2 years, 4 months
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Question about the alcohol sponge inoculation tek
#18851708 - 09/16/13 10:37 PM (10 years, 4 months ago) |
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I was wondering about this tek. I'm very new to cultivating, but I've done a good bit of research. I was thinking a glovebox is the best way as of right now but I just stumbled upon this and was winding why everyone doesn't use this for pf. I would like to know if there any cons like the alcohol affecting the substrate or anything else. Is it a great idea or no? http://www.shroomery.org/11402/Fast-easy-fool-proof-inoculation
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Question about the alcohol sponge inoculation tek [Re: mrbeanstalk]
#18851728 - 09/16/13 10:42 PM (10 years, 4 months ago) |
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Alcohol sanitizes, it doesn't sterilize. Just flame the needle red hot and squirt a drop out to cool it down.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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mrbeanstalk
Starter


Registered: 03/03/13
Posts: 81
Last seen: 2 years, 4 months
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Re: Question about the alcohol sponge inoculation tek [Re: mushmagic]
#18851756 - 09/16/13 10:49 PM (10 years, 4 months ago) |
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But do you thing that the whole alcohol sponge idea is "foolproof" you have your flame sterilized needle going thru the sponge to inoculate. So there's no really chance for airborne spores to get in the jar?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Question about the alcohol sponge inoculation tek [Re: mrbeanstalk]
#18851779 - 09/16/13 10:55 PM (10 years, 4 months ago) |
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Quote:
RogerRabbit said: Wiping a tool with alcohol after flame sterilizing is like wiping shit on your face after taking a shower. RR
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Question about the alcohol sponge inoculation tek [Re: PussyFart]
#18851784 - 09/16/13 10:56 PM (10 years, 4 months ago) |
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Flame it red hot, don't ever wipe it with anything after flaming it red hot.
Nothing is more sterile than red hot.
If "airborne contams" are getting into your jars when you are inoculating, you need to work on your sterile procedures.
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mrbeanstalk
Starter


Registered: 03/03/13
Posts: 81
Last seen: 2 years, 4 months
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Re: Question about the alcohol sponge inoculation tek [Re: FrankHorrigan]
#18851823 - 09/16/13 11:06 PM (10 years, 4 months ago) |
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And another question would be how affective would you say the dry layer of verm is to protect the jar. And how is it protecting it. i inoculated in a box with holes lysoled everything around wore gloves wiped everything down. Flamed the needle and wiped the needle in between jars with a disinfectant wet wipe.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: Question about the alcohol sponge inoculation tek [Re: mrbeanstalk]
#18851831 - 09/16/13 11:09 PM (10 years, 4 months ago) |
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Quote:
mrbeanstalk said: And another question would be how affective would you say the dry layer of verm is to protect the jar. And how is it protecting it.
Very.
It remains dry and contams cannot colonize/make thru it.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
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Re: Question about the alcohol sponge inoculation tek [Re: mrbeanstalk]
#18851832 - 09/16/13 11:09 PM (10 years, 4 months ago) |
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You should be flaming needles before each jar. The vermiculite barrier is extremely effective, as can be seen by the thousands of successful grows using PF Tek.
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