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Offlinenaturesrevolt
StrangerOfAwesome
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Registered: 05/27/11
Posts: 681
Loc: Missouri Flag
Last seen: 9 months, 24 days
A bad spore printing job somehow worked, explain?
    #18851014 - 09/16/13 08:12 PM (10 years, 4 months ago)

I took a spore print from 4 mushrooms. I let them sit on aluminum foil and made foil tents for them to stay moist in. Then the unsterilized conditions started. I took a pot of water to 180F (mid-sized bubbles) and turned it off for a few moments. Then I dripped some water onto the foil and lifted the spores into the solution. This solution came out so dark it was black (the spores are violet). After that I used 4 syringes and cleaned them out with the heated water once before sucking up the solution into an unused jar and making 48ml of spore material.
I expected this to fail, but I inoculated 10 jars with a syringe and they all grew fine (quite more than fine).
After reading posts on here, I know sterile conditions must be present to get good spores. So why did it work so well in such filthy conditions?
Also, pic of the syringes.


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
Re: A bad spore printing job somehow worked, explain? [Re: naturesrevolt]
    #18851037 - 09/16/13 08:17 PM (10 years, 4 months ago)

Prints aren't usually going to be sterile since the fruits were grown in open air.  You simply want to be as clean as you possibly can be.  Using an aluminum foil tent simulates an SAB, and although it's not the greatest or cleanest way to possibly take prints, it obviously worked for you.

You can use half pint jars with a filter and silicone port to sterilize water and then sterilize your syringes with the water, and pull sterile water into the syringes so you aren't using 180*F water which could harm the spores.

Now, about those syringes... Those things are dark, when using them, you probably won't need a full mL for a jar, simply drop the smallest amount of solution you can into the jars.


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Offlinenaturesrevolt
StrangerOfAwesome
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Registered: 05/27/11
Posts: 681
Loc: Missouri Flag
Last seen: 9 months, 24 days
Re: A bad spore printing job somehow worked, explain? [Re: Mush4Brains]
    #18851868 - 09/16/13 11:20 PM (10 years, 4 months ago)

yeah, I know I could replicate the syringes with sterile water and things will be fine. I just only had those 4 at the time.


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