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OfflineLTSwoomz
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Registered: 02/16/13
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Contaminated Grain Spawn!
    #18850195 - 09/16/13 05:05 PM (10 years, 4 months ago)

What up thugs? Knocked up a few jars on the 5th and haven't seen any growth till the last two days. I went to shake because I saw growth and noticed that one of them contaminated. I think it's bacteria. It was in a really big clump, pics below, but for sure a contam. Details of the process below. I'm guessing I didn't get the moisture content right with the WBS. This thing was snugly stuck to the side of the jar, I had to beat it (haha) pretty hard to get it loose. Details on my procedure are below the pics.



WBS was done via Frank's guide. Rinsed thoroughly, floaters removed, soaked for ~20 - 24 hours, heated and stirred till it begins to simmer then taken off and dried out over the course of an hour. Paper towel test was successful. Load into the jar, PC'd at 15psi for 90m.

Jars were knocked up in a GB SAB, needle was flame sterilized then poked through an ISO soaked (but squeezed out) sponge (there's a guide around here somewhere for it). This particular jar was shaken right after innoc for testing purposes.

Going to toss the jar, wash it, sterilize it, start another. I still have 3 in there, one was done the same way just not shaken and so far doesn't appear to have contams. The other two were knocked up over the course of this last week and done almost the same but with more attention paid to moisture content after the soak.


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Edited by LTSwoomz (09/16/13 05:06 PM)


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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: Contaminated Grain Spawn! [Re: LTSwoomz]
    #18850232 - 09/16/13 05:14 PM (10 years, 4 months ago)

In light of some recent conversations with trusted members, I'm going to go out on a limb and say it was your spores or inoculation technique.

If you PCed properly, there should be no contams in your jar, even if it's a little too wet.

I think the culprit is sticking your flame sterilized needle in a unsterilized sponge.


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OfflineLTSwoomz
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Re: Contaminated Grain Spawn! [Re: SpitballJedi]
    #18850250 - 09/16/13 05:18 PM (10 years, 4 months ago)

Quote:

SpitballJedi said:
In light of some recent conversations with trusted members, I'm going to go out on a limb and say it was your spores or inoculation technique.

If you PCed properly, there should be no contams in your jar, even if it's a little too wet.

I think the culprit is sticking your flame sterilized needle in a unsterilized sponge.




Ok thanks man, I'll look into PCing my tools before use then, any other takers or is this pretty much for sure what the problem is?

EDIT: Also, the sponge sits in ISO for about 5 min before being used, it's completely submerged, is this not adequate?


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Edited by LTSwoomz (09/16/13 05:19 PM)


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InvisibleSpitballJedi
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Re: Contaminated Grain Spawn! [Re: LTSwoomz]
    #18850274 - 09/16/13 05:25 PM (10 years, 4 months ago)

Not really. ISO only sanitizes. There's no telling what kind of nasties are in that sponge and ISO will not take care of it.

I think it was RR who said something like "sticking your flame sterilized needle in a ISO soaked sponge is like washing you face and then drying it with a shitty diaper" or something like that.

Every jar I ever made using that tek contaminated. I went through 21 jars.

When I used to use SHIPS, I had best results when I stuck my red hot needle right in the ship and flamed between each jar. But my ships did not last very long.


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OfflineLTSwoomz
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Re: Contaminated Grain Spawn! [Re: SpitballJedi]
    #18850391 - 09/16/13 05:54 PM (10 years, 4 months ago)

Quote:

SpitballJedi said:
Not really. ISO only sanitizes. There's no telling what kind of nasties are in that sponge and ISO will not take care of it.

I think it was RR who said something like "sticking your flame sterilized needle in a ISO soaked sponge is like washing you face and then drying it with a shitty diaper" or something like that.

Every jar I ever made using that tek contaminated. I went through 21 jars.

When I used to use SHIPS, I had best results when I stuck my red hot needle right in the ship and flamed between each jar. But my ships did not last very long.




Interesting. Thanks. What do you use instead of SHIPs?


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InvisibleSpitballJedi
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Re: Contaminated Grain Spawn! [Re: LTSwoomz]
    #18850445 - 09/16/13 06:05 PM (10 years, 4 months ago)

Agar. I don't use syringes anymore.


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OfflineLTSwoomz
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Re: Contaminated Grain Spawn! [Re: SpitballJedi]
    #18857150 - 09/18/13 09:12 AM (10 years, 4 months ago)

I was reading through some material on sterile ways to inoculate and randomly came across the post you quoted, how awesome is that? It was by Notahacker though. Just found it too awesome that I randomly found it shortly after you mentioned it.

Quote:

Notahacker420 said:
Quote:

TieACable2aTree said:
Is it possible for the red hot syringe to ignite the alcohol when it comes in contact with the soaked sponge?



I doubt it, but wiping a flame sterilized needle with an unsterile substance like alcohol is like wiping shit on your face after a shower.




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InvisibleDoc_D
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Registered: 11/13/12
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Re: Contaminated Grain Spawn! [Re: LTSwoomz]
    #18857620 - 09/18/13 11:44 AM (10 years, 4 months ago)

Quote:

LTSwoomz said:
I was reading through some material on sterile ways to inoculate and randomly came across the post you quoted, how awesome is that? It was by Notahacker though. Just found it too awesome that I randomly found it shortly after you mentioned it.

Quote:

Notahacker420 said:
Quote:

TieACable2aTree said:
Is it possible for the red hot syringe to ignite the alcohol when it comes in contact with the soaked sponge?



I doubt it, but wiping a flame sterilized needle with an unsterile substance like alcohol is like wiping shit on your face after a shower.








Par for the course...          :wink:


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Offlinelateforthafuture
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Re: Contaminated Grain Spawn! [Re: Doc_D]
    #18861653 - 09/19/13 05:21 AM (10 years, 4 months ago)

Just out of curiosity are you letting your pressure cooker cool naturally and by that I mean letting it cool and not taking the regulator off until all the pressure has released, when I first started grains I made that mistake and had a ton of bacterial contains then someone gave me that advice and all my contains stopped. If you pull the regulator off before all the pressure is released it creates a vacuum and can suck contains into your jars


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