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Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineBL00MERS
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First Time LC ?'s based on pictures
    #18849005 - 09/16/13 12:47 PM (10 years, 4 months ago)

Thanks in advance for any and all help I receive. I just completed my flow hood and have begun to expanded my practices to G2G and the use of LC's and agar. This is my first LC, and looking for some insight from mycologists with LC experience. They are QT jars with a .2 micron filtration whatman filter and self healing injection port, attached with High-temp RTV. The medium is ELME boiled into distilled water and then PC'ed at 15PSI for 35 minutes. I followed TL's Simple LC Tek. These were inoculated 6 days ago, with 1cc from a MS syringe I've had stored in the fridge for approximately 7 months. Kept in an incubator at around 82-84 degrees. I am curious if these show healthy mycelium growth and if they are ready to use to inoculate grain. Also, in the picture with less growth, compared to the rest, there is a slightly darker color and I am unsure what this could be. Knocking up some test BRF 1/2 pints today. Thanks!




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Happy growing!


Edited by BL00MERS (09/16/13 01:04 PM)


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OfflinePussyFart
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Re: First Time LC ?'s based on pictures [Re: BL00MERS]
    #18849109 - 09/16/13 01:09 PM (10 years, 4 months ago)

There is no way to visually confirm 100% if an LC is good or not.

Test jars must be shot up and grown out...


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OfflineBL00MERS
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Re: First Time LC ?'s based on pictures [Re: PussyFart]
    #18849122 - 09/16/13 01:12 PM (10 years, 4 months ago)

Understood...thanks for the quick response. I will update with pictures/info from the test jars.


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Happy growing!


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InvisiblebodhisattaMDiscordReddit
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Re: First Time LC ?'s based on pictures [Re: BL00MERS]
    #18849177 - 09/16/13 01:26 PM (10 years, 4 months ago)

You can make mini pf cakes with shotglasses to test your lc and they look cool when fruited.


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InvisiblePestile

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Re: First Time LC ?'s based on pictures [Re: bodhisatta]
    #18849187 - 09/16/13 01:28 PM (10 years, 4 months ago)

Yeah, sorta like a bonsai tree. :smile:


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The Corbett Report
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OfflineBL00MERS
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Re: First Time LC ?'s based on pictures [Re: Pestile]
    #18849345 - 09/16/13 02:07 PM (10 years, 4 months ago)

Never even considered a shot glass, I'll give that a go...thanks for the great idea!


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OfflineBL00MERS
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Re: First Time LC ?'s based on pictures [Re: BL00MERS]
    #18867480 - 09/20/13 12:31 PM (10 years, 4 months ago)

Inoculated some 1/2 pint test jars following the basic PF Tek to see how my LC's are doing. As far as the shot glass/mini pf cakes, how would I go about sterilizing that in a PC? Just a double layer of foil over the top? I usually just work with metal lids, and plastic lids on occasion...starting to move away from plastic lids. I have tyvek, whatman filters, SFD's, self healing injection ports. I have a flowhood and dedicated clean room... pretty much anything I would need, so anybody that has done mini pf cakes please let me know how it should be done. Thanks! Shroomery fucking rocks!!


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Happy growing!


Edited by BL00MERS (09/20/13 12:41 PM)


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InvisibleFooMan
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Re: First Time LC ?'s based on pictures [Re: BL00MERS]
    #18867593 - 09/20/13 12:47 PM (10 years, 4 months ago)

Double foil for lids works. I would still use a dry verm layer to help filter out contaminants.


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Quick WBS Prep


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OfflineBL00MERS
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Re: First Time LC ?'s based on pictures [Re: FooMan]
    #18867649 - 09/20/13 12:59 PM (10 years, 4 months ago)

Right on, thanks FooMan.


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Happy growing!


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