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OfflineDeadPhan
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Registered: 05/05/04
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Last seen: 4 years, 2 months
Can I move agar to grain earlier than later?
    #18844666 - 09/15/13 01:56 PM (10 years, 4 months ago)

Do I have to wait for it to grow out or is it ok for me to transfer a wedge now.  It's about 50 percent. What's the benefit to letting it grow it if I'm not isolating but only planning on moving to agar?  Ill get some pics soon


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
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Re: Can I move agar to grain earlier than later? [Re: DeadPhan]
    #18844675 - 09/15/13 02:01 PM (10 years, 4 months ago)

Sure you can use it. Just try to take as little bare agar as possible for your wedge.


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OfflineDeadPhan
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Re: Can I move agar to grain earlier than later? [Re: FrankHorrigan]
    #18844688 - 09/15/13 02:06 PM (10 years, 4 months ago)

I meant moving to grain. What do you mean by as little bare agar as possible?


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
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Re: Can I move agar to grain earlier than later? [Re: DeadPhan]
    #18844697 - 09/15/13 02:08 PM (10 years, 4 months ago)

I know you meant move it to grain.

Cut a circle around the mycelium then slice your wedges (if you are making wedges).

Do not use the "bare" agar that is uncolonized when moving wedges to grains.


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Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinefatflash
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Registered: 01/15/13
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Last seen: 21 days, 13 hours
Re: Can I move agar to grain earlier than later? [Re: FrankHorrigan]
    #18844709 - 09/15/13 02:12 PM (10 years, 4 months ago)

Think he means cut away only the mycelium when transferring I.e cut away the bare agar??

Am I right on this? Bare agar in your grains can cause contams? ?


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InvisibleFrankHorrigan
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Re: Can I move agar to grain earlier than later? [Re: fatflash]
    #18844717 - 09/15/13 02:14 PM (10 years, 4 months ago)

It won't cause contams if you are using good sterile procedure...or rather, it won't cause contams if you don't have anything land on it while you're moving the wedge to the jar. But that applies to the whole wedge.

The bare agar serves zero purpose for inoculating grains. It does however provide a bigger "landing pad" for contams that may be in the air when you inoculate.

Does this make sense?


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Can I move agar to grain earlier than later? [Re: FrankHorrigan]
    #18844951 - 09/15/13 03:07 PM (10 years, 4 months ago)

Agar is sticky. Uncolonized agar will easily pull things from the air. Way easier than it'll just fall into your open jar.

Yes, you can use your agar now. You're never supposed to let it run all the way to the edge anyway.


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i recommend common sense and figuring it out.

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