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Shop: Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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Offlinepatate
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Registered: 08/12/13
Posts: 5
Last seen: 10 years, 5 months
Question about inoculation
    #18844506 - 09/15/13 01:06 PM (10 years, 5 months ago)

Hello,

I was wondering if, when sterilizing the needle with a flame until red hot, after that, you have to let a drop of the solution come out of the syringe in order to cool it BEFORE or AFTER inserting the needle into the jar (I am trying WBS jars with polyfill and high temp silicone on the tops, so it was not a problem to insert the needle into the silicone). Since it has been a couple of days since I inoculated my jars and I didn't seen any sign of colonization yet, I thought that could have been where I messed it up (I let the jars cooled to room temp 24 hours after pc them).

Thanks a lot!!


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OfflineStromriderM
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Registered: 06/02/13
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Re: Question about inoculation [Re: patate]
    #18844540 - 09/15/13 01:16 PM (10 years, 5 months ago)

Before...Well it actually doesn't matter

The soonest you will ever see growth is 3 days and that is rare. It is usually 5 to 7 days before you see growth but can take as much as 2 weeks


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OfflinePrinceShroom
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Registered: 04/01/13
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Re: Question about inoculation [Re: patate]
    #18844542 - 09/15/13 01:18 PM (10 years, 5 months ago)

Put it into the jar and jut shoot however much your using into it.  It doesnt really matter which way you do it.  I personally put it in and just inoculate


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OfflineStromriderM
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Registered: 06/02/13
Posts: 7,338
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Last seen: 13 hours, 44 minutes
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Re: Question about inoculation [Re: PrinceShroom]
    #18844550 - 09/15/13 01:20 PM (10 years, 5 months ago)

Quote:

PrinceShroom said:
Put it into the jar and jut shoot however much your using into it.  It doesnt really matter which way you do it.  I personally put it in and just inoculate




Agreed


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