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OfflineNakor420
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Registered: 05/02/13
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Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube.
    #18840240 - 09/14/13 07:16 AM (10 years, 5 months ago)

Mystery because I lost track of which culture this was and the label came off, so this is either Mazatapec, Cambodian, B+, or Golden Teacher



Again...once this is 100% I will transfer to grains...this is the start of another great thread.  Enjoy. :smile:


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OfflineDeadPhan
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Registered: 05/05/04
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Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: Nakor420]
    #18842712 - 09/14/13 10:23 PM (10 years, 5 months ago)

How many transfers is this from?  What method of inoculation?


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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Invisibleprovo
Peace Pilgrim

Registered: 10/18/09
Posts: 189
Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: DeadPhan]
    #18842744 - 09/14/13 10:38 PM (10 years, 5 months ago)

Nice isolate.

Just keep in mind that if this is not from a clone and simply an isolate from ms you may have poor fruiting performance and you truly can't know until you have fruited it. So fruit it and let us all see!

Unlikely though :tongue2:

Following:happyheart:


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OfflineDeadPhan
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Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: provo]
    #18842959 - 09/14/13 11:58 PM (10 years, 5 months ago)

I'm just getting into agar.  I'm confuse as to why anyone would isolate ms on agar. I've got my first run of ms on agar.  I've made one transfer so far. I've been debating on isolating but I don't see the point as per the chance of non fruitiness. Is this to say ms grown on agar, however not yet isolated might have a better chance at fruiting considering multiple strains are at work?


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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Offlineomegafaust
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Registered: 05/29/12
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Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: DeadPhan]
    #18842965 - 09/15/13 12:01 AM (10 years, 5 months ago)

Possibly, but you will not have the consistency that you get from an isolate.


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The Universe has an interesting sense of irony, in that you are the universe experiencing itself.  All you are is a thought.


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OfflineNakor420
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Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: omegafaust]
    #18843402 - 09/15/13 04:44 AM (10 years, 5 months ago)

IT's like 3 transfers .... got up today and noticed my culture had a friend in there......



Which sucks because I wanted to use this culture for inoculating grains...now I have to transfer away from a stupid contaminate....:facepalm3:

Actually now that I look back at the first photo you can see it there too, just barely.


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Edited by Nakor420 (09/15/13 04:52 AM)


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OfflineStromriderM
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Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: Nakor420]
    #18843417 - 09/15/13 05:10 AM (10 years, 5 months ago)

Quit playing with it so much and it wouldn't have got contamed! :lol:


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OfflineNakor420
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Registered: 05/02/13
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Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: Stromrider]
    #18843422 - 09/15/13 05:15 AM (10 years, 5 months ago)

Quote:

psillyshroomer said:
Quit playing with it so much and it wouldn't have got contamed! :lol:







:hehehe:


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: Stromrider]
    #18843429 - 09/15/13 05:23 AM (10 years, 5 months ago)

Looks great thus far.  It looks like you could possibly make a few isolates from this plate, and it may be a good plan to do that.  Test 3-4 isolates for fruits. 

I don't have the link handy, but Notahacker420 has a spectacular write up "The tale of 10 isolates" in his signature.  He did a bang-up job showing how isolates handle themselves, and some of the pitfalls. 

So far so good! 

Quote:

psillyshroomer said:
Quit playing with it so much and it wouldn't have got contamed! :lol:



Contamination is not from playing with it.  Contamination come from sharing it with cheap dates.  You go blind from playing with it;)


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OfflineNakor420
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Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 10 years, 23 days
Re: Still some slight sectoring but I think this is isolated enough for me. This is a mystery cube. [Re: HypnotoadCroaked]
    #18843434 - 09/15/13 05:26 AM (10 years, 5 months ago)

Yeah, I see about 3 good sectors there still...I'm already preparing my petris... :smile:


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