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TheGreenBox

Registered: 08/03/13
Posts: 276
Last seen: 4 years, 2 months
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Inoculation: Bathroom or kitchen?
#18843138 - 09/15/13 01:37 AM (10 years, 4 months ago) |
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I've read that a small bathroom with no drafts that can easily be cleaned and sprayed with bleach is best for inoculation. I've also seen sources say you should try to use a fume hood.
I have a small bathroom I can quickly clean, or a kitchen with an overhead suction fan above the oven that acts like a "fume hood". Just wondering what would be best for inoculation, or does it really matter?
I will also be using a glovebox and being very clean in general, of course.
Thanks!
-------------------- And in my darkest moment, fetal and weeping, the moon tells me a secret - my confidant. As full and bright as I am, this light is not my own and a million light reflections pass over me. Its source is bright and endless. - Tool
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: Inoculation: Bathroom or kitchen? [Re: TheGreenBox]
#18843148 - 09/15/13 01:44 AM (10 years, 4 months ago) |
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It won't matter where you are if your using a glovebox or still air box and following proper sterile procedure. I have never heard of a fume hood. Enlighten a nuckah?
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: Inoculation: Bathroom or kitchen? [Re: TheGreenBox]
#18843149 - 09/15/13 01:44 AM (10 years, 4 months ago) |
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The answer is in a SAB.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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TheGreenBox


Registered: 08/03/13
Posts: 276
Last seen: 4 years, 2 months
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Re: Inoculation: Bathroom or kitchen? [Re: PussyFart]
#18843153 - 09/15/13 01:46 AM (10 years, 4 months ago) |
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Quote:
DeadPhan said: It won't matter where you are if your using a glovebox or still air box and following proper sterile procedure. I have never heard of a fume hood. Enlighten a nuckah?
Fume hood used for extracting air from the workspace and filtering it. Seems to be some debate about whether or not this is good.
Quote:
Notahacker420 said: The answer is in a SAB.
I assume SAB = Still Air Box? Yeah, I have that. So it shouldn't make much of a difference?
-------------------- And in my darkest moment, fetal and weeping, the moon tells me a secret - my confidant. As full and bright as I am, this light is not my own and a million light reflections pass over me. Its source is bright and endless. - Tool
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: Inoculation: Bathroom or kitchen? [Re: PussyFart]
#18843154 - 09/15/13 01:46 AM (10 years, 4 months ago) |
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Quote:
Notahacker420 said: The answer is in a SAB.

K. Now were even.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Inoculation: Bathroom or kitchen? [Re: TheGreenBox]
#18843156 - 09/15/13 01:47 AM (10 years, 4 months ago) |
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I think what you're referring to is a flow hood. A SAB (still air box) will work just fine though.
SAB's work much better for this hobby than A GB (glovebox) by the way.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: Inoculation: Bathroom or kitchen? [Re: TheGreenBox]
#18843157 - 09/15/13 01:48 AM (10 years, 4 months ago) |
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This, fume hood is soooo not good. Who told you of this silly method?
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: Inoculation: Bathroom or kitchen? [Re: mushmagic]
#18843159 - 09/15/13 01:49 AM (10 years, 4 months ago) |
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Quote:
mushmagic said: I think what you're referring to is a flow hood. A SAB (still air box) will work just fine though.
SAB's work much better for this hobby than A GB (glovebox) by the way.
I thought they were one in the same. No?
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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TheGreenBox


Registered: 08/03/13
Posts: 276
Last seen: 4 years, 2 months
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Re: Inoculation: Bathroom or kitchen? [Re: DeadPhan]
#18843172 - 09/15/13 01:53 AM (10 years, 4 months ago) |
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Hmm weird. Yeah the fume hood idea seems a bit sketchy due to air currents.
Also, as DeadPhan said, isn't a GB the same as SAB? Basically what I have is a box with small holes for my arms, and it is completely sealed from the outside environment.
-------------------- And in my darkest moment, fetal and weeping, the moon tells me a secret - my confidant. As full and bright as I am, this light is not my own and a million light reflections pass over me. Its source is bright and endless. - Tool
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Inoculation: Bathroom or kitchen? [Re: DeadPhan]
#18843176 - 09/15/13 01:54 AM (10 years, 4 months ago) |
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Quote:
DeadPhan said:
Quote:
mushmagic said: I think what you're referring to is a flow hood. A SAB (still air box) will work just fine though.
SAB's work much better for this hobby than A GB (glovebox) by the way.
I thought they were one in the same. No?
No a GB has gloves and tends to create drafts as you move your arms and you also can't flame your untencils because the gloves are attached while a SAB just has holes so you can take you're hands out to flame your utencils and you don't create drafts from moving your hands with attached gloves nor from the heat of trying to flame inside the box (and possibly causing danger if you use iso to clean it). SAB's a far superior for this purpose.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Re: Inoculation: Bathroom or kitchen? [Re: TheGreenBox]
#18843610 - 09/15/13 07:53 AM (10 years, 4 months ago) |
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Bathrooms and kitchens are the two filthiest rooms in any house. Don't use either for sterile work.
Fume hoods draw air into and through the work space to protect the operator. Flow hoods blow filtered air on the work space to protect the culture. Use the latter or a still air box. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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TheGreenBox


Registered: 08/03/13
Posts: 276
Last seen: 4 years, 2 months
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Re: Inoculation: Bathroom or kitchen? [Re: RogerRabbit]
#18843778 - 09/15/13 09:14 AM (10 years, 4 months ago) |
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Quote:
RogerRabbit said: Bathrooms and kitchens are the two filthiest rooms in any house. Don't use either for sterile work.
RR
Thank you RR. Much appreciated.
So I should instead work in a different room with a SAB? I read that carpeted rooms are bad... this true?
Thanks again!
-------------------- And in my darkest moment, fetal and weeping, the moon tells me a secret - my confidant. As full and bright as I am, this light is not my own and a million light reflections pass over me. Its source is bright and endless. - Tool
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spiz
Stranger

Registered: 05/17/11
Posts: 118
Loc: Australia
Last seen: 2 years, 1 month
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Re: Inoculation: Bathroom or kitchen? [Re: RogerRabbit]
#19559082 - 02/12/14 08:24 PM (9 years, 11 months ago) |
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Quote:
RogerRabbit said: Bathrooms and kitchens are the two filthiest rooms in any house. Don't use either for sterile work.
Fume hoods draw air into and through the work space to protect the operator. Flow hoods blow filtered air on the work space to protect the culture. Use the latter or a still air box. RR
Kitchen bathroom or a carpeted bedroom which would be the best? A SAB will be used. A lot of experienced growers seem to have good results using any of these options
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: Inoculation: Bathroom or kitchen? [Re: spiz]
#19559152 - 02/12/14 08:37 PM (9 years, 11 months ago) |
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Quote:
spiz said: Kitchen bathroom or a carpeted bedroom which would be the best?
Answer.....
Quote:
spiz said: A SAB
Period.
You could be in a mold filled room, but with good sterile technique and a SAB you can come out a winner.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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spiz
Stranger

Registered: 05/17/11
Posts: 118
Loc: Australia
Last seen: 2 years, 1 month
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Re: Inoculation: Bathroom or kitchen? [Re: PussyFart]
#19559161 - 02/12/14 08:39 PM (9 years, 11 months ago) |
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Exactly what I was thinking rr just putting doubts in my head haha
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Re: Inoculation: Bathroom or kitchen? [Re: spiz]
#19559281 - 02/12/14 09:12 PM (9 years, 11 months ago) |
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It's best to work in a clean area away from kitchens or bathrooms. They're the two rooms with the highest mold and bacteria counts of any room in your house. At least start your still air box in a cleaner area since they're not airtight by any means. Clean out a closet in the bedroom and use that. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
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Re: Inoculation: Bathroom or kitchen? [Re: RogerRabbit]
#19559286 - 02/12/14 09:13 PM (9 years, 11 months ago) |
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Bathroom, Kitchen, County Jail Holding Cell..
All the same.
Do it up.
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The ego is nothing other than the focus of conscious attention.
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spiz
Stranger

Registered: 05/17/11
Posts: 118
Loc: Australia
Last seen: 2 years, 1 month
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Re: Inoculation: Bathroom or kitchen? [Re: RogerRabbit]
#19559292 - 02/12/14 09:14 PM (9 years, 11 months ago) |
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Hmm so carpet is better than the other two. Would not have thought that Thank you!
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
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Re: Inoculation: Bathroom or kitchen? [Re: spiz]
#19559316 - 02/12/14 09:19 PM (9 years, 11 months ago) |
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I constructed a stand out of 2x3's and I use it for My SAB and my fruiting chamber. All of my work is done in a closet
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