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OfflineFarmerwhite
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How do you sterilize syringes and tools in an SAB?
    #18840894 - 09/14/13 12:01 PM (10 years, 5 months ago)

Do you just use a flame of some sort?


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OfflineTrippy_Penguin
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Re: How do you sterilize syringes and tools in an SAB? [Re: Farmerwhite]
    #18840910 - 09/14/13 12:05 PM (10 years, 5 months ago)

Flame them outside of the box. Use an alcohol burner or a cigarette lighter.

Flame needles and tools until they are red hot. You can squirt a few drops of liquid through the needle to cool it prior to inserting it into jars. Flame between every jar.


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Offlinerew736
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Re: How do you sterilize syringes and tools in an SAB? [Re: Trippy_Penguin]
    #18840914 - 09/14/13 12:07 PM (10 years, 5 months ago)

Syringes I use flame.
Tools I use peroxide or bleach solution...


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malicom said:
"Banana's emit radiation. In fact banannas are the standard in detecting solar radiation. The radiation read from a bananna tells scientists how active the sun was during the growing season. Make sure to steer clear of bananas too."
seekaytea said:
"I need to stop spending all my money on plants and take up a cheaper hobby, like heroin."
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InvisibleFrankHorrigan
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Re: How do you sterilize syringes and tools in an SAB? [Re: rew736]
    #18841064 - 09/14/13 01:05 PM (10 years, 5 months ago)

I keep my tools inside of a jar (in the SAB) filled with isopropyl alcohol.

I take them out as needed, remove them from the SAB and hit them with a small blowtorch until red-hot.

Then quickly and carefully move it back inside the SAB and proceed :thumbup:


--------------------

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You should take a look. :hehehe:


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OfflineTheStormsEye
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Re: How do you sterilize syringes and tools in an SAB? [Re: FrankHorrigan]
    #18841118 - 09/14/13 01:27 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
I keep my tools inside of a jar (in the SAB) filled with isopropyl alcohol.

I take them out as needed, remove them from the SAB and hit them with a small blowtorch until red-hot.

Then quickly and carefully move it back inside the SAB and proceed :thumbup:




Are you saying you take a tool covered in alcohol and hit it with a flame,
while holding it with a gloved hand??
I gotta see this, haha

:onfire:


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InvisibleFrankHorrigan
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Re: How do you sterilize syringes and tools in an SAB? [Re: TheStormsEye]
    #18841125 - 09/14/13 01:29 PM (10 years, 5 months ago)

:house:

Obviously the tool is only covered in iso up to a certain point. I use a tall half-pint, not a quart jar :lol:

I've yet to burn myself and I've done a few hundred transfers and a few dozen inoculations since I added the ISO jar a few weeks back :wink:

It is great, it's a double-whammy vs. contams on your tools.


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Offlinerew736
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Re: How do you sterilize syringes and tools in an SAB? [Re: FrankHorrigan]
    #18841171 - 09/14/13 01:48 PM (10 years, 5 months ago)

:ilold:


--------------------
My trade list
malicom said:
"Banana's emit radiation. In fact banannas are the standard in detecting solar radiation. The radiation read from a bananna tells scientists how active the sun was during the growing season. Make sure to steer clear of bananas too."
seekaytea said:
"I need to stop spending all my money on plants and take up a cheaper hobby, like heroin."
Wiccan_Seeker said:
"Your knowledge on male-on-male rape and willingness to engage in fantasy regarding the matter is commendable."
eminemvs.slimshady said:
"I want to know if they are active and magic or not. And if they are not active, can i pick them before they are active and then they will become active or do they have to grow till they activate?"


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OfflineFarmerwhite
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Re: How do you sterilize syringes and tools in an SAB? [Re: rew736]
    #18841180 - 09/14/13 01:53 PM (10 years, 5 months ago)

But do not flame syringes inside the box?


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InvisibleFrankHorrigan
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Re: How do you sterilize syringes and tools in an SAB? [Re: Farmerwhite]
    #18841192 - 09/14/13 01:59 PM (10 years, 5 months ago)

Dont flame anything inside the SAB.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
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InvisibleLeopardMan
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Re: How do you sterilize syringes and tools in an SAB? [Re: FrankHorrigan]
    #18841215 - 09/14/13 02:12 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
I keep my tools inside of a jar (in the SAB) filled with isopropyl alcohol.




No offense but that's what I call going overboard :wink:


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OfflineTheStormsEye
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Re: How do you sterilize syringes and tools in an SAB? [Re: LeopardMan]
    #18841222 - 09/14/13 02:15 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
Dont flame anything inside the SAB.




Yes indeed, this would cause air to be drawn into the box
due to the flame consuming oxygen

Also, you risk burning yourself and/or melting or setting ur SAB on fire.
Haha, none of which is a good thing..

Quote:

LeopardMan said:
Quote:

FrankHorrigan said:
I keep my tools inside of a jar (in the SAB) filled with isopropyl alcohol.




No offense but that's what I call going overboard :wink:




At least tools stay somewhat clean before he is ready to use em..
:shrug:


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OfflineMush4Brains
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Re: How do you sterilize syringes and tools in an SAB? [Re: LeopardMan]
    #18841227 - 09/14/13 02:16 PM (10 years, 5 months ago)

Quote:

LeopardMan said:
Quote:

FrankHorrigan said:
I keep my tools inside of a jar (in the SAB) filled with isopropyl alcohol.




No offense but that's what I call going overboard :wink:




Nice to see you LM.


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InvisibleLeopardMan
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Re: How do you sterilize syringes and tools in an SAB? [Re: TheStormsEye]
    #18841239 - 09/14/13 02:21 PM (10 years, 5 months ago)

If you flame your tools there is no need to use isopropyl alcohol :shrug:

@Mush4Brains :hatsoff:


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InvisibleFrankHorrigan
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Re: How do you sterilize syringes and tools in an SAB? [Re: LeopardMan]
    #18841552 - 09/14/13 04:17 PM (10 years, 5 months ago)

I think it was fastfred who suggested it to me.

It's not like I think it does anything the flame doesn't do :lol:

It does have the nice side effect of getting any crap off my scalpel before flaming.

And since the tools would be sitting in the empty jar anyhow, I figured why not? :shrug:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
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OfflineSockadin
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Re: How do you sterilize syringes and tools in an SAB? [Re: FrankHorrigan]
    #18841725 - 09/14/13 05:27 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
:house:

Obviously the tool is only covered in iso up to a certain point. I use a tall half-pint, not a quart jar :lol:

I've yet to burn myself and I've done a few hundred transfers and a few dozen inoculations since I added the ISO jar a few weeks back :wink:

It is great, it's a double-whammy vs. contams on your tools.



:aweohyou:



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InvisiblebodhisattaMDiscordReddit
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Re: How do you sterilize syringes and tools in an SAB? [Re: Sockadin]
    #18841965 - 09/14/13 06:37 PM (10 years, 5 months ago)

Doesn't pulling your hand in an out of your SAB make more airflow than a torch inside? I only use warm soapy water to spray the inside instead of ISO so that it doesn't go boom. I haven't had a problem with contams flaming inside of a box but if you guys really think I should do it the other way I'll give it a try.


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InvisibleHypnotoadCroaked
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Re: How do you sterilize syringes and tools in an SAB? [Re: bodhisatta]
    #18842002 - 09/14/13 06:46 PM (10 years, 5 months ago)

I've taken to using a pint and a half (3/4th quart) widemouth full of tools (2 knives, half dozen inoculation loops, qtips, aluminum foil) and pressure cooked with grain.  I still flame till red hot, but I don't mind a bit of redundancy. 

On a side note, I am loving fitting 8 of these pint and a half jars full of millet through my PC in contrast to 7 full sized quarts.


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InvisibleFrankHorrigan
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Re: How do you sterilize syringes and tools in an SAB? [Re: bodhisatta]
    #18842255 - 09/14/13 07:58 PM (10 years, 5 months ago)

Quote:

bodhisatta said:
Doesn't pulling your hand in an out of your SAB make more airflow than a torch inside? I only use warm soapy water to spray the inside instead of ISO so that it doesn't go boom. I haven't had a problem with contams flaming inside of a box but if you guys really think I should do it the other way I'll give it a try.




No, not really. But it's not about airflow really. You should let the air settle once you move your tools back inside regardless. On the flipside, you'll need to let the air settle after flaming inside the box.

That heat will create a lot more air movement than you would think.

And if you do a test on a petri dish, you can see that your sterile red-hot blade or needle will still be sterile after being moved back into the SAB :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineFarmerwhite
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Re: How do you sterilize syringes and tools in an SAB? [Re: FrankHorrigan]
    #18843051 - 09/15/13 12:37 AM (10 years, 5 months ago)

So is an SAB wish 4' holes flipped top down with tyvek sleeves attached ( anyone have the best way to attach them?) sufficient for inoculation, g2g, and agar?


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InvisibleHypnotoadCroaked
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Re: How do you sterilize syringes and tools in an SAB? [Re: Farmerwhite]
    #18843316 - 09/15/13 03:33 AM (10 years, 5 months ago)

Quote:

Farmerwhite said:
So is an SAB wish 4' holes flipped top down with tyvek sleeves attached ( anyone have the best way to attach them?) sufficient for inoculation, g2g, and agar?



http://www.shroomery.org/forums/usergallery.php?gallery=273258

Photo courtesy of Notahacker420.  If using tyvek sleeves, some tape them onto the inside of the fitting.  I have seen zip-tied variations as well.  Gloves and holes work perfect for me.

Yes, the Sab is sufficient for all of the work you described.


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