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OfflineJackOfNoTrades
Person of Interest

Registered: 07/30/13
Posts: 121
Last seen: 10 years, 2 months
Inject Through the Tape Or Pull it back???
    #18841568 - 09/14/13 04:26 PM (10 years, 4 months ago)

Hey, I'm about to make a second run with jars using brf pf tek. I did it both ways last time and it didn't seem to matter. And of course I will re-flame the needle between jars.

My question is do you inject through the micropore tape or do you peel it back then inoculate?

If you inject through the tape do you put more tape over top of it? Is two layers of micropore tape a "bad thing"?


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InvisibleThe Psyentist
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Registered: 07/30/13
Posts: 82
Loc: Central Florida Flag
Re: Inject Through the Tape Or Pull it back??? [Re: JackOfNoTrades]
    #18841571 - 09/14/13 04:28 PM (10 years, 4 months ago)

I don't use any tape and have no issues.


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OfflineNakor420
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Registered: 05/02/13
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Re: Inject Through the Tape Or Pull it back??? [Re: The Psyentist]
    #18841575 - 09/14/13 04:29 PM (10 years, 4 months ago)

The dry verm layer is your filter...so it doesn't matter...:pipesmoke:


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
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Re: Inject Through the Tape Or Pull it back??? [Re: Nakor420]
    #18841580 - 09/14/13 04:31 PM (10 years, 4 months ago)

It does matter.

You can carry contams from the tape into the cake when you use the needle to puncture it.

Remove the tape first.


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Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineNakor420
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Re: Inject Through the Tape Or Pull it back??? [Re: FrankHorrigan]
    #18841582 - 09/14/13 04:32 PM (10 years, 4 months ago)

:whathesaid: When you put it like that...yeah I guess it does matter,lol.


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OfflineOneiricOutlier
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Registered: 09/11/13
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Re: Inject Through the Tape Or Pull it back??? [Re: FrankHorrigan]
    #18841660 - 09/14/13 05:02 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
You can carry contams from the tape into the cake when you use the needle to puncture it.



Isn't the tape already sterilized after being in the PC though?


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OfflineMush4Brains
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Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
Re: Inject Through the Tape Or Pull it back??? [Re: OneiricOutlier]
    #18841668 - 09/14/13 05:04 PM (10 years, 4 months ago)

Quote:

I did it both ways last time and it didn't seem to matter




You have your answer.


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Invisiblecynical bastad
another guy
Registered: 08/17/13
Posts: 175
Re: Inject Through the Tape Or Pull it back??? [Re: Mush4Brains]
    #18841711 - 09/14/13 05:23 PM (10 years, 4 months ago)

I've been trying to find all the little questions too:

Quote:

RogerRabbit said:
You're supposed to remove the foil as soon as you remove the jars from the sterilizer.  The foil does not stop contaminants.  It is only there to prevent the water which 'rains' from the lid of the pot from getting into the jars through the inoculation holes.  If you leave the foil on, the verm layer can't dry out as it's supposed to.  Wet verm is food for fungi, so contaminants will colonize right through it.

Micropore tape gives an extra measure of protection, and since it breathes it can be left on.  Wipe it with alcohol and inject the red-hot needle right through.  Re-flame for each jar.  Watch it all done in the video link below.
RR




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OfflineJackOfNoTrades
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Re: Inject Through the Tape Or Pull it back??? [Re: cynical bastad]
    #18841794 - 09/14/13 05:50 PM (10 years, 4 months ago)

I like having the tape on there. It just makes me feel so much better. Thank you for the advice. I just find these little tiny questions are best to have answered as one tiny microbe or mistake can cost you everything.


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InvisibleFruitOfLife
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Registered: 05/21/12
Posts: 4,832
Re: Inject Through the Tape Or Pull it back??? [Re: JackOfNoTrades]
    #18842005 - 09/14/13 06:48 PM (10 years, 4 months ago)

This is a great question. Never thought about until now, but I have always just left the tape on.

I might try taking it off before inoculating a few times and see if there is any difference


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Invisiblecynical bastad
another guy
Registered: 08/17/13
Posts: 175
Re: Inject Through the Tape Or Pull it back??? [Re: FruitOfLife]
    #18842160 - 09/14/13 07:28 PM (10 years, 4 months ago)

I've only actually did it the way FH mentioned above, but i'm extremely new to it all, and trying to have all my answers before hand this time. I'm just able to relay information i've noticed by pulling up quotes. I am not speaking from experience like any of these guys. It's all making since and leading to more questions...hah If using a thicker gauged needle, heating it red hot and sticking micro spore tape...or any tape...will it cause it to possibly melt a bit and slide? Creating a bigger hole? Or would just putting another piece of tape on top solve that?

Making it more difficult than it actually is should be my quote...


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OfflineJackOfNoTrades
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Posts: 121
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Re: Inject Through the Tape Or Pull it back??? [Re: cynical bastad]
    #18842213 - 09/14/13 07:47 PM (10 years, 4 months ago)

Okay here is the other conundrum that is one of those little questions. I probably should repost this as a separate question but I think I started a good thread here.

Let's say I shoot through the tape. I obviously have punctured the tape if I did this. If a second layer of micropore is put over top the original will that effect FAE in a negative manner or make no difference? Two layer of mircropore tape.

I know the verm is a natural barrier, but I see no drawbacks to using tape to just a little more added protection against contams or dust than may settle into the lid holes. I don't know, i'm new to this, these are my thoughts... I love constructive criticism I learn so much from the kind members on this site everyday.


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Invisiblecynical bastad
another guy
Registered: 08/17/13
Posts: 175
Re: Inject Through the Tape Or Pull it back??? [Re: JackOfNoTrades]
    #18842246 - 09/14/13 07:55 PM (10 years, 4 months ago)

I'm not sure on BRF or PF Tek, are you supposed to have poly fill or a filter disk for GE like grains? I dig these threads and the members too, soooo much knowledge.


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
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Re: Inject Through the Tape Or Pull it back??? [Re: cynical bastad]
    #18842290 - 09/14/13 08:07 PM (10 years, 4 months ago)

Consider the jars to not be sterile the moment they move through open air / you open your PC.

Unless you do so in aseptic conditions like in front of a hood.

So sure, you can wipe it with iso just like a self healing inoc port. But I don't like the sticky crap on the micropore and I don't like any added risk. The needle going through anything but a blank hole can cause contams to "ride" in.

Best to omit the tape completely, trust the dry verm layer and inoculate through the open holes. No need to worry about it then.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineJackOfNoTrades
Person of Interest

Registered: 07/30/13
Posts: 121
Last seen: 10 years, 2 months
Re: Inject Through the Tape Or Pull it back??? [Re: FrankHorrigan]
    #18842420 - 09/14/13 08:45 PM (10 years, 4 months ago)

FH,
If you steam the jars without tape only tin foil wont excess moisture get in the jar?

I understand the verm layer is a barrier but does it hurt to but a little tape over the top for add protection? Does it effect FAE to the point it's not worth it?


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InvisibleFrankHorrigan
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Posts: 10,573
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Re: Inject Through the Tape Or Pull it back??? [Re: JackOfNoTrades]
    #18842496 - 09/14/13 09:09 PM (10 years, 4 months ago)

Excess moisture will get in even with the tape.

The jar will "steam" dry the verm layer on top when you remove them from the PC and remove the foil.

I agree the extra protection will not hurt and it will provide plenty of GE.

But if you're going to put micropore tape over the holes, do it after you inoculate.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisibletbagtag
Boomer Barron

Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam Flag
Re: Inject Through the Tape Or Pull it back??? [Re: JackOfNoTrades]
    #18842507 - 09/14/13 09:12 PM (10 years, 4 months ago)

Unless you're using a tiny needle when you stick it through the tape you're going to make a nice size hole, sometimes too hot of a needle will also melt the tape a bit rendering it rather useless.  The dry verm layer as long as you dont pick up your jar and fuck with it will serve its purpose.  double tinfoil the top if you're that worried.


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OfflineJackOfNoTrades
Person of Interest

Registered: 07/30/13
Posts: 121
Last seen: 10 years, 2 months
Re: Inject Through the Tape Or Pull it back??? [Re: FrankHorrigan]
    #18842523 - 09/14/13 09:17 PM (10 years, 4 months ago)

FC,

So when you steam or PC your jars you just tin foil the top? No tape? You only apply tape after the inn?


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Inject Through the Tape Or Pull it back??? [Re: JackOfNoTrades]
    #18842531 - 09/14/13 09:18 PM (10 years, 4 months ago)

I don't apply the tape at all.

It serves no purpose IMO. The dry verm layer is your filter :wink:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineJackOfNoTrades
Person of Interest

Registered: 07/30/13
Posts: 121
Last seen: 10 years, 2 months
Re: Inject Through the Tape Or Pull it back??? [Re: FrankHorrigan]
    #18842545 - 09/14/13 09:21 PM (10 years, 4 months ago)

FC,

So when you steam or PC your jars you just tin foil the top? No tape? You only apply tape after the inn?


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