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drek
Stranger


Registered: 09/14/13
Posts: 2
Last seen: 10 years, 4 months
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What is this golden brown liquid in my jars?
#18841190 - 09/14/13 01:57 PM (10 years, 4 months ago) |
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I got a lot of information from reading this forum, and I feel it's time to ask for some advice. Growing these things is a like having a puppy for the first time, it's easy until yellow and brown stuff comes of of it.
In my little kitchen table operation I have 4 rye grain jars, inoculated with a spore syringe, that I planned to grow for a few months for the stones. Each jar has a 1/2 inch hole in the lid filled with synthetic fiber for air exchange.
The jars are ~2 months old and not fully colonized yet.
In the beginning it was summer, 80 F in the daytime and about 68 F at night, so I kept the jars outside for 1 month. Then the temperature dropped and condensation started to form inside the jars, so I put them in a kind of incubator with constant 79 F.
The incubator accelerated growth a lot and condensation dropped after increasing temperature.
A few days ago I noticed some golden, brown, yellow (amber colored) droplets on the surface of the substrate. I had previously seen a pale yellowish discoloration of the white mycelium, but I read on the forums that it was normal and just the "metabolism" of the fungus.
I then lowered the temperature to 75 F, but that made condensation increase. And it seems that more and more of the brown, yellow liquid is forming around certain areas in the jars. Yesterday I raised the temperature back to 79 F.
Where I live the temperature can change a lot and in the winter I have about 60-65 F indoors, so I imagine that I need the incubator. The spore vendor also recommended 79 F.
When I pick up the jars they are hot (hotter than 79 F), they are like little animals, sweating and excreting weird stuff.
The question I am hoping to have answered is this: How are they doing? Is the liquid forming inside my jars a problem? What are golden droplets on the mycelium? Is the fried-egg-like clumps normal? Is there something I can or should do to decrease the formation of brown liquid?
When I inoculated the jars, I believe the grains had a little bit too much moisture in them. Next time I will dry them out some more before cooking them.
The quality of the pictures is not the finest, but they are taken with my only and best digital camera. The pictures that are mostly cloudy white, is supposed to show the golden droplets on top of the substrate. I hope I am not the only one who can see them.
Thanks in advance! And thank you for all the helpful material on this site.
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Mr. Wilson
experienced weiner rubber



Registered: 12/19/11
Posts: 1,977
Loc: Weinerville,
Last seen: 3 years, 1 month
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Re: What is this golden brown liquid in my jars? [Re: drek]
#18841203 - 09/14/13 02:06 PM (10 years, 4 months ago) |
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in pcs 6 10 and 11 you seem to have a little sclerotia action going on--that liquid is metabolites due to a bacterial contam though---and in the future you should try not fucking with em so much--i.e. leave em in the dark and leave em alone---just my 2 cents---im sure other more experienced growers will chime in and id listen to them more than me---what are they BTW (strainwise)---and what vendor actually discussed cultivation with you---if they are from here disregard that ???
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spore baby



Registered: 07/30/13
Posts: 4,918
Last seen: 8 years, 7 months
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Re: What is this golden brown liquid in my jars? [Re: drek]
#18841238 - 09/14/13 02:21 PM (10 years, 4 months ago) |
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.
Edited by spore baby (12/27/14 01:46 AM)
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drek
Stranger


Registered: 09/14/13
Posts: 2
Last seen: 10 years, 4 months
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Re: What is this golden brown liquid in my jars? [Re: Mr. Wilson]
#18841247 - 09/14/13 02:23 PM (10 years, 4 months ago) |
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Mexicana A.
The yellow stuff has shown up recently, about the time I put them in the incubator.
So, do I toss them and start over?
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Mr. Wilson
experienced weiner rubber



Registered: 12/19/11
Posts: 1,977
Loc: Weinerville,
Last seen: 3 years, 1 month
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Re: What is this golden brown liquid in my jars? [Re: drek]
#18841280 - 09/14/13 02:36 PM (10 years, 4 months ago) |
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an "incubator" has no use in this hobby and its very existence is born from impatientness---when you put your jars in that high temp environment you made a perfect situation for bacteria to thrive----BUT you are getting SOME action---don't know if I would throw them away---well unless you wanted to grow anything in the future---then I would have got rid of em like yesterday--lol
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Trippy_Penguin



Registered: 03/18/12
Posts: 624
Last seen: 3 years, 5 months
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Re: What is this golden brown liquid in my jars? [Re: Mr. Wilson]
#18841376 - 09/14/13 03:10 PM (10 years, 4 months ago) |
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Quote:
Mr. Wilson said: an "incubator" has no use in this hobby and its very existence is born from impatientness
Untrue. A well built incubator can be very useful and really speed up colonization times.
If a well built incubator causes contams, you need to look at your sterile procedure.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 13 hours, 15 minutes
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Re: What is this golden brown liquid in my jars? [Re: Trippy_Penguin]
#18841669 - 09/14/13 05:06 PM (10 years, 4 months ago) |
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Quote:
If a well built incubator causes contams, you need to look at your sterile procedure.
Sterilization time more than procedure if it seems to be strictly temperature related contamination.
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