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TheLoopIs
Registered: 05/29/13
Posts: 330
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What to do with syringe after inoculation to ensure future use?
#18840427 - 09/14/13 09:06 AM (10 years, 4 months ago) |
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After I have inoculated my jars should I replace the needle with the blue cap back on the syringe? I feel like doing this might get contaminates in the grooves of the syringe where the needle and the body connect. How do you treat a syringe after you have inoculated with it for future use? (Can't PC it because I'm still saving spore solution inside of the syringe)
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audiophoenix
Find Peace



Registered: 08/28/09
Posts: 4,107
Loc: Upstate NY
Last seen: 2 years, 8 months
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Re: What to do with syringe after inoculation to ensure future use? [Re: TheLoopIs]
#18840445 - 09/14/13 09:11 AM (10 years, 4 months ago) |
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First, I try to always make a full go of my syringe so that I'm not left with a half used syringe. But I've been there.
Basically if I'm planning on using it again I'll take the cap off the needle and place it with the opening down on a paper towel with some alcohol on it to prevent mold spores from getting in. when I'm done with it I'll just sterilize the needle and replace the cap. That's it.
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Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 9 years, 11 months
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Re: What to do with syringe after inoculation to ensure future use? [Re: audiophoenix]
#18840462 - 09/14/13 09:16 AM (10 years, 4 months ago) |
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I just slip the cap back on and put it in a baggy in the fridge...it's fine as long as you flame sterilize your needle properly next time and wipe the outside of it with iso. I do it all the time...no problems ever
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tbagtag
Boomer Barron

Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam
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Re: What to do with syringe after inoculation to ensure future use? [Re: Nakor420]
#18840524 - 09/14/13 09:47 AM (10 years, 4 months ago) |
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Fill it with smack and hotline it!
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Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 9 years, 11 months
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Re: What to do with syringe after inoculation to ensure future use? [Re: tbagtag]
#18840592 - 09/14/13 10:19 AM (10 years, 4 months ago) |
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Quote:
tbagtag said: Fill it with smack and hotline it!
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Nimpo
Big Black


Registered: 05/10/12
Posts: 2,375
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Re: What to do with syringe after inoculation to ensure future use? [Re: Nakor420]
#18840674 - 09/14/13 10:50 AM (10 years, 4 months ago) |
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Ive left half used syringes for later use all the time.
Just reflame the needle before you cap it.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: What to do with syringe after inoculation to ensure future use? [Re: TheLoopIs]
#18840684 - 09/14/13 10:54 AM (10 years, 4 months ago) |
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Flame sterilize the needle until it's glowing then remove it from the flame and immediately press the syringe plunger down slightly until you hear a little sizzle from the spore solution coming up through the needle and cap the needle.
The flame will kill contaminants and the spore solution coming through the needle will immediately cool it. You shouldn't wipe the needle with alcohol after flame sterilizing since the wipe wouldn't be sterile.
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Quick WBS Prep
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TheLoopIs
Registered: 05/29/13
Posts: 330
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Re: What to do with syringe after inoculation to ensure future use? [Re: FooMan]
#18840767 - 09/14/13 11:21 AM (10 years, 4 months ago) |
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too awesome thank u for all the amazing tips earthlings
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Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 9 years, 11 months
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Re: What to do with syringe after inoculation to ensure future use? [Re: TheLoopIs]
#18840877 - 09/14/13 11:57 AM (10 years, 4 months ago) |
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Yeah, I didn't mean flame then wipe the NEEDLE with iso, I meant the outside of the syringe it's self.
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