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Offlinemudbutt
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Isolating fluffy mycelium?
    #18832059 - 09/12/13 11:17 AM (10 years, 4 months ago)

Sorry I don't have any pics I am new at agar and isolating a strain so I have a question ......... I have some agar dishes that are completely colonized all aver the agar with multi spore  .....but it is all fluffy mycelium can I transfer a small piece of the  mycelium to another plate and hope that it turns to a  rizarmorphic type mycelium? Is that Possible?





Also an unrelated question.....I have some spore prints that I am pretty sure are contaminated ....If I make a couple of syringes out of it and dilute the crap out of it with a bunch of water do you think I will have a better chance of getting a couple of good jars out of it?  Thanks guys for your help!!  :smile:     






Here is something to listen to while you think about my questions


Edited by mudbutt (09/12/13 11:23 AM)


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Offlinekinkaku
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Re: Isolating fluffy mycelium? [Re: mudbutt]
    #18832124 - 09/12/13 11:33 AM (10 years, 4 months ago)

Quote:

Sorry I don't have any pics I am new at agar and isolating a strain so I have a question ......... I have some agar dishes that are completely colonized all aver the agar with multi spore  .....but it is all fluffy mycelium can I transfer a small piece of the  mycelium to another plate and hope that it turns to a  rizarmorphic type mycelium? Is that Possible?






you may have to do a few transfers before you even start to see rizo growth. so just transfer the strongest growth you see first:thumbup:

Quote:

Also an unrelated question.....I have some spore prints that I am pretty sure are contaminated ....If I make a couple of syringes out of it and dilute the crap out of it with a bunch of water do you think I will have a better chance of getting a couple of good jars out of it?  Thanks guys for your help!!       






no, if you think they are contaminated either throw them out or put them to agar.


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OfflineStromriderM
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Re: Isolating fluffy mycelium? [Re: kinkaku]
    #18832157 - 09/12/13 11:45 AM (10 years, 4 months ago)

Yeah man if you have agar what are you worried about possibly contamed spore prints for? Any spore print taken outside of a lab environment is possibly contaminated


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OfflineLingabhakt
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Re: Isolating fluffy mycelium? [Re: Stromrider]
    #18883957 - 09/24/13 09:00 AM (10 years, 4 months ago)

Contaminated prints, huh? I hope not, lol. :wink:
:pipesmoke:Good luck with your agar, though. I have never tried it, but it sounds like fun.


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Edited by Lingabhakt (09/24/13 09:03 AM)


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Offlinemudbutt
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Re: Isolating fluffy mycelium? [Re: Lingabhakt]
    #18895264 - 09/26/13 06:22 PM (10 years, 4 months ago)

these are some print someone sent me....the ones im sending you are good :smile:


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OfflineLingabhakt
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Re: Isolating fluffy mycelium? [Re: mudbutt]
    #18897941 - 09/27/13 09:49 AM (10 years, 4 months ago)

I have no doubt. I was just being a jackass.  :dumblol:


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OfflineBuzzbee88
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Re: Isolating fluffy mycelium? [Re: kinkaku]
    #27343864 - 06/11/21 02:02 AM (2 years, 7 months ago)

My mycelium shows no rhizomorphic growth so far I'm going to give it about 2 days before transferring... is there any way to promote the growth of rhizomorphic strands?



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OfflineDERRAYLD
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Re: Isolating fluffy mycelium? [Re: Buzzbee88]
    #27343877 - 06/11/21 02:09 AM (2 years, 7 months ago)

Next time focus your search using the advanced searched and search for threads younger than 5 years to get up to date info.
It's not really necessary to isolate rhizo vs tormentose growth, you're after a clean uniform culture, that is all.


Edited by DERRAYLD (06/11/21 02:10 AM)


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