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Novanity1
Student


Registered: 08/29/10
Posts: 326
Last seen: 9 years, 1 month
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Bad luck with Copelandias
#18830729 - 09/11/13 11:58 PM (10 years, 4 months ago) |
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So I bought two different strains from the copelandia genus (pan cambo and pan cyan) from a vendor recently and have had zero luck so far. First I put a drop of spore solution to agar with each syringe. With the pan cambo I saw immediate growth. It was strong and white but very tomentose. It almost had an orange hue to it which I was very suspicious about but just dismissed it as the color of the agar. The cyan dish on the other hand showed no growth whatsoever.
I knocked 3 rye jars up. 1 with cambo MS, 1 cyan ms, and 1 from the cambo agar dish. The cambo agar took of the quickest and showed some rather promising white growth. However when I open the jar to spawn it, it had a very pungent sour/sweet smell. Having no experience with copelandias I spawned it anyways just for the hell of it. It contamed obviously. The Cambo MS took off but then showed lots of funky grey growth so I tossed it. The Cyan showed very little growth then turned into more gray contams.
I wanted to dismiss this failure as bad syringes but I figured there's no way both were contamed especially since this particular vendor has always delivered the goods.
So I tried again...

Here's what I have currently for the Cambo MS (knocked up on 9/6/13). I tried WBS this time thinking maybe my methods were flawed with rye berries.
How does it look? It definitely looks whiter and stronger than the first attempt!

Here's what I have for my second attempt at Cyans. Notice I knocked this one up on 8/1/13? I'm assuming it doesn't take this long to colonize so it must be contamed. However it looks totally fine, just very week.
My 3rd Cyan attempt I did on WBS as opposed to eye as well. However there is no germinaton yet cuz it's only been 5 days. I'll post pics if I see any germination on that jar.
I've researched a lot on copelandias and they seem relatively easy to grow. I'm just not used to seeing cope mycelium let alone how it smells so I'm a tad apprehensive.
All input is welcomed.
Thanks
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MacMerdin
Hunter



Registered: 04/26/12
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Re: Bad luck with Copelandias [Re: Novanity1]
#18831172 - 09/12/13 04:44 AM (10 years, 4 months ago) |
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From what I can see, they look ok.
If you have more spores, you might want to check my signature for a simple way to get pans to fruit in a SGFC.
Good luck to you.
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: Bad luck with Copelandias [Re: MacMerdin]
#18831910 - 09/12/13 10:22 AM (10 years, 4 months ago) |
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I've had that "grey" growth (2nd pic) recently with Sandose; did it colonize the jar really fast? I'm still not sure what it was, perhaps cobweb, but it looked more like light, wispy mycelium 
Pan mycelium does smell different than cube mycelium - more like a sharpie marker in my limited experience.
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Novanity1
Student


Registered: 08/29/10
Posts: 326
Last seen: 9 years, 1 month
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Re: Bad luck with Copelandias [Re: mycomattie]
#18832593 - 09/12/13 02:00 PM (10 years, 4 months ago) |
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The second pic is taking wayyyy too long to colonize. It's been over a month and all I got is this incredibly weak/whispy growth. Sharpie smell is an interesting way to put it. I've heard others say it smells "musky". I know that the sour apple smell could not possibly be a good smell though.
If anyone is feeling generous and has an abundance of pan prints PM me
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Novanity1
Student


Registered: 08/29/10
Posts: 326
Last seen: 9 years, 1 month
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Re: Bad luck with Copelandias [Re: MacMerdin]
#18832608 - 09/12/13 02:03 PM (10 years, 4 months ago) |
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Quote:
MacMerdin said: From what I can see, they look ok.
If you have more spores, you might want to check my signature for a simple way to get pans to fruit in a SGFC.
Good luck to you. 
I saw that thread a while back and favorited it!
Very cool approach to pans. I might have to give that a try if I have any lucky getting some healthy pan mycelium.
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Novanity1
Student


Registered: 08/29/10
Posts: 326
Last seen: 9 years, 1 month
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Re: Bad luck with Copelandias [Re: Novanity1]
#18837679 - 09/13/13 04:40 PM (10 years, 4 months ago) |
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bumb
Anyone else have some input?
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Sagescruffy
CH



Registered: 10/30/09
Posts: 2,011
Loc: PNW
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Re: Bad luck with Copelandias [Re: Novanity1]
#18837906 - 09/13/13 05:44 PM (10 years, 4 months ago) |
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How many transfers did you make on agar? If you got good spore syringes and innoc'd through a self healing injection port, then I don't see why you'd fail. I did however notice that you left the sunflower seeds in your wbs. In my experience they usually come out when the seeds get rinsed. So I'm under the impression that you didn't rinse your seeds. I'm not saying that not rinsing your seeds would cause things to contam, but it reveals a little bit (to me anyway) about how much you care about preparing things etc
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Novanity1
Student


Registered: 08/29/10
Posts: 326
Last seen: 9 years, 1 month
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Re: Bad luck with Copelandias [Re: Sagescruffy]
#18838080 - 09/13/13 06:26 PM (10 years, 4 months ago) |
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Quote:
Sagescruffy said: How many transfers did you make on agar? If you got good spore syringes and innoc'd through a self healing injection port, then I don't see why you'd fail. I did however notice that you left the sunflower seeds in your wbs. In my experience they usually come out when the seeds get rinsed. So I'm under the impression that you didn't rinse your seeds. I'm not saying that not rinsing your seeds would cause things to contam, but it reveals a little bit (to me anyway) about how much you care about preparing things etc
I only did one transfer. And yes I rinsed the crap out of my WBS I just prefer to leave the sunflower seeds in. I've never had an issue with contams because of the sunflower seeds and I've seen TC's leave them in as well. Although the first 2 contams I got were with rye which is Im trying WBS this round. Not that my grain choice has anything to do with it I just figured I'd change some sort of variable to see what results I get.
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SexySmurff
HyperQube



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Re: Bad luck with Copelandias [Re: Novanity1]
#18981522 - 10/15/13 02:11 PM (10 years, 3 months ago) |
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You aren't the only one to have gotten this orange growth.
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Giggle_Grower
A lil less noob each day



Registered: 04/07/10
Posts: 1,598
Loc: Shroomery
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Re: Bad luck with Copelandias [Re: SexySmurff]
#18981883 - 10/15/13 03:42 PM (10 years, 3 months ago) |
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Ive gotten the orange growth twice in a few yrs. Once recently in a BRF jar. And once in a SGFC a while back. No one every had a clue what it was. The one in a SG was like a orange cobweb, that spread over the entire chamber in in a mattet of 12 hrs. I havent seen it since and dont want to. Tho i wish I had taken pics of them. Back to the OP, good luck with your pans. I havent tried them yet but will be shortly. Ill watch and learn from this thread.
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Novanity1
Student


Registered: 08/29/10
Posts: 326
Last seen: 9 years, 1 month
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Sorry guys I haven't had internet for over a month so I've been off the shroomery for a while. Lots of things going on: foreclosure scares, lawyers, bass lights, roommates moving out new one just moved in etc....
I wish I took pictures of the progress but like I said I've been busy 
The Cambo jar actually fully colonized with some healthy looking growth. However at about the 90-100% colonization range it started to get a slight orange hue. When I opened the jar, sure enough, it had the same sour apple smell. In my desperate attempts to get some god damn cope fruits for once in my life I spawned it anyways lol. It colonized the sub in about 10 days and still had that faint orange hue to it but the sour smell was a lot less intense. I applied my casing layer and ended up getting green mold and cobweb on top so I tossed it out 
I wanna say I messed up during the pasteurization of the casing layer but the 5 other trays in my GH have totally contam free casing layers that were pasteurized in the same batch with the same type of jars and everything so It must have been the spawn.
Not really sure what to think since I've never seen or smelled cope myc in person. At this point I'm convinced I simply got some bum syringes.
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