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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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10 days of colonizing PE's. Only 30% so far. Tips?
#18824137 - 09/10/13 04:31 PM (10 years, 4 months ago) |
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HI Everyone! I am new here to posting on shroomery but definitely not new to the site. The information off these forums has helped me grow a few strains in the past with the brown rice flour technique and I have had a lot of luck. I now just moved up to WBS. I did twelve one pint jars(since I didn't have qts) and did not experience one contamination. All jars were nice and white in about two and a half weeks. I have since spawned into a bulk substrate using Damions coir/verm tek. It has been ten days since I spawned. this afternoon I took the trash bag of from around and looked in. There is massive Myc' growth on the sides by where I put my polyphil and in the corners, little spots in the middle that are nice and thick, almost has the appearance of knotting, but other spots there are is no Myc' coming through. All together I would say it's about thirty percent colonized on the top casing layer which i didn't make even an inch thick. I was thinking since I failed to cover the holes with tape and just poly'd them, that the CO2 must be settling close to the outside of the tub and seeping out of the holes causing most of the colonization around the edges. I have since taped the holes and re-covered the tub with a blanket i sprayed with lysol. Going to check in another few days. I also have ALOT of condensation and I dabbed some of it up with a paper towel, just the big drops. So do you guys think its the CO2 not pooling right or just slow colonization because of the strain being Penis Envy genetics? Also the thick myc doesnt appear to be Triche. its not cotony but thick and shiny like the outside layer of a cake looks.
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18824221 - 09/10/13 04:51 PM (10 years, 4 months ago) |
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HI Everyone! I am new here to posting on shroomery but definitely not new to the site. The information off these forums has helped me grow a few strains in the past with the brown rice flour technique and I have had a lot of luck. I now just moved up to WBS. I did twelve one pint jars(since I didn't have qts) and did not experience one contamination. All jars were nice and white in about two and a half weeks. I have since spawned into a bulk substrate using Damions coir/verm tek. It has been ten days since I spawned. this afternoon I took the trash bag of from around and looked in. There is massive Myc' growth on the sides by where I put my polyphil and in the corners, little spots in the middle that are nice and thick, almost has the appearance of knotting, but other spots there are is no Myc' coming through. All together I would say it's about thirty percent colonized on the top casing layer which i didn't make even an inch thick. I was thinking since I failed to cover the holes with tape and just poly'd them, that the CO2 must be settling close to the outside of the tub and seeping out of the holes causing most of the colonization around the edges. I have since taped the holes and re-covered the tub with a blanket i sprayed with lysol. Going to check in another few days. I also have ALOT of condensation and I dabbed some of it up with a paper towel, just the big drops. So do you guys think its the CO2 not pooling right or just slow colonization because of the strain being Penis Envy genetics? Also the thick myc doesnt appear to be Triche. its not cotony but thick and shiny like the outside layer of a cake looks.
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masonwinston
just a guy


Registered: 06/19/13
Posts: 57
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18824959 - 09/10/13 07:44 PM (10 years, 4 months ago) |
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Dude, PE takes for fucking ever. You'll see it posted on the forums, by regulars and TCs, and it's 100% the truth. Be patient, hang tight, and have faith.
If your sub was 100% colonized when you added your casing layer, and the casing layer is now roughly 30% colonized, start fruiting.
Remain calm, smoke a bowl, watch the SoA season premiere this evening. You'll be picking dicks before you know it!
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: masonwinston]
#18825000 - 09/10/13 07:50 PM (10 years, 4 months ago) |
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Yeah Penis Envy can be a BIATCH when using multi-spore bro. But it'll be worth it, you will probably get a bunch of mutants too. Notorious for weird little guys, try cloning a specimen on agar when you get a desirable fruit...
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masonwinston
just a guy


Registered: 06/19/13
Posts: 57
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Quote:
nn-IlliniSpiralDMT said: Yeah Penis Envy can be a BIATCH when using multi-spore bro. But it'll be worth it, you will probably get a bunch of mutants too...
Ah, but he's applied a casing layer! My PE tub just started fruiting, about two weeks after I took off the tape and poly-filled. The cool thing is - no mutants, no blobs, no anything weird. Per Frank's suggestion, I cased my sub, hoping to avoid weird shit. So far, so good.
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Smeagol
Poke my 3rd eye


Registered: 04/14/13
Posts: 319
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Threads clear back to '09 talk aboutPE taking a long time. Also several experienced growers have been posting to correct the idea CO2 settles or drains. It doesn't. Both RR and FH have posted about it. RR even took measurements and the readings were highest from the top holes where the warmer air, warmed by the heat produced by the myc, would exit.
-------------------- The road to hell is paved with good intentions, but it is not paved with clarity.” -Terence "If you're not peaking what the fuck are you doing?" Dude on facebook
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: masonwinston]
#18827188 - 09/11/13 10:36 AM (10 years, 4 months ago) |
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I mixed the spawn directly in the pasteurized substrate evenly then applied roughly 3/4 inch casing layer composed of the coir/verm mix. There is thick myc all the way around the edges and almost a solid 2 inch or more stripe across the center with little thick patches in between. So do you think I should fruit? Looked again today and the center patch has thickened it appears but I have not taken any pictures to reference. In case you're wondering my temp is about 74 degrees ambient temp and very high humidity in my area. I figure it should be around 77 in the tub but I dropped my digital thermometer in the water in the outside tub of my incubator a couple weeks ago and keep forgetting to buy a new one. Temp in the house really doesn't fluctuate so I haven't felt to much the need to buy another yet I guess.
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masonwinston
just a guy


Registered: 06/19/13
Posts: 57
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18828969 - 09/11/13 05:36 PM (10 years, 4 months ago) |
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Yes, I think you should fruit it.
Your casing layer should be as non-nutritive as possible, so coir/verm wasn't the best choice. That's a great bulk substrate combo, it's exactly what I'm using, but you should case with something else. You could very well be looking at a situation where you end up with blobs/mutants, because instead of a casing layer, you just added another 3/4" of substrate.
Related: your casing layer should be about 1/2", so maybe use less next time. For my 66 qt. tub, I used 3 qts. of the Jiffy 50/50+ mentioned below, and that did the trick for me.
When you apply your casing layer, don't press it down or compact it. You want to crumble it over the top of the substrate, so that it's a semi-fine consistency. I wore latex gloves, and scooped handfuls of 50/50+ out of my pasteurization bag. I then moved my hands over my open tub and rubbed them together, finely crumbling the casing mix over my substrate. It's really that easy.
From Frank's "Casing PE" thread:
Quote:
After considering a lot of anecdotal evidence I've seen over the years, I cased my MS penis envy substrates at 100% using pasteurized 50/50+. For this 50/50+ mix I used jiffy mix organic seed starter, right from the bag. It was hydrated to field capacity and pasteurized, allowed to cool and then applied.
Admission of inexperience: I'm not far enough along in my mycological development to do much more than parrot what's said by the Shroomery's top contributors, so I can't fully explain what I'm telling you.
Keep at it, man, you'll get it! Growing mushrooms is definitely NOT rocket surgery (or brain science).
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Novanity1
Student


Registered: 08/29/10
Posts: 326
Last seen: 9 years, 1 month
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18829091 - 09/11/13 06:09 PM (10 years, 4 months ago) |
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Quote:
DaFun.Guy said: HI Everyone! I am new here to posting on shroomery but definitely not new to the site. The information off these forums has helped me grow a few strains in the past with the brown rice flour technique and I have had a lot of luck. I now just moved up to WBS. I did twelve one pint jars(since I didn't have qts) and did not experience one contamination. All jars were nice and white in about two and a half weeks. I have since spawned into a bulk substrate using Damions coir/verm tek. It has been ten days since I spawned. this afternoon I took the trash bag of from around and looked in. There is massive Myc' growth on the sides by where I put my polyphil and in the corners, little spots in the middle that are nice and thick, almost has the appearance of knotting, but other spots there are is no Myc' coming through. All together I would say it's about thirty percent colonized on the top casing layer which i didn't make even an inch thick. I was thinking since I failed to cover the holes with tape and just poly'd them, that the CO2 must be settling close to the outside of the tub and seeping out of the holes causing most of the colonization around the edges. I have since taped the holes and re-covered the tub with a blanket i sprayed with lysol. Going to check in another few days. I also have ALOT of condensation and I dabbed some of it up with a paper towel, just the big drops. So do you guys think its the CO2 not pooling right or just slow colonization because of the strain being Penis Envy genetics? Also the thick myc doesnt appear to be Triche. its not cotony but thick and shiny like the outside layer of a cake looks.
I'm having a little trouble following exactly what you did... Pictures would really really help.
It's good that you covered the holes up with tape because the sub is not supposed to have FAE during colonization. You need GE, there is a big difference between the two. I would ditch the blanket and just put the lid over it. The lid is not air tight so it should provide enough GE. A blanket sounds like it would provide way too much FAE.
What do you mean by casing layer? Did you put a layer of peat/verm over the top? Or is it just a layer of more coir/verm. Either way, you shouldn't have done either.
There is no need to put a layer of more coir/verm. Just mix the substrate and WBS up evenly and don't worry about covering the top because it won't contam. I would even soak your WBS up to 20 minutes before you spawn it. I get 100% colonization in as little as 3 days sometimes by dunking my grains.
If you did use a layer of peat/verm then you put it on too soon. Casing layers are to be applied after 100% colonization then wait for the casing layer to colonize 20%
Also, the damion bucket tek is a little outdated. It's not the best means of pasteurization and could leave parts of your sub unpasteurized which could be a reason for the lack of colonization.
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: Novanity1]
#18832262 - 09/12/13 12:27 PM (10 years, 4 months ago) |
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I didnt know about the casing layer needing to be different then the substrate. Yes the layer is probably around a half inch no more then three quarters. I pretty much put a bottom layer of sub, then broke up my spawn over it. I mixed it together and then added the little top layer/ I do have the lid on it but a blanket over that to help the temp stay up a degree or two and keep and ambient light out because the tub is not completely enclosed but in a closet elevated but light is able to get in the closet cause of a lack of a door and me improvising. Definitely helpful advice. We will see how it does with this top layer I put. should i try and remove any of it or just leave it as is. I am going to check the tub and maybe take a pic later if i can get a camera to show you guys and get the final word on moving to fruiting
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masonwinston
just a guy


Registered: 06/19/13
Posts: 57
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18832433 - 09/12/13 01:15 PM (10 years, 4 months ago) |
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Do not remove the top layer you applied; leave it alone. It'll set you back and retard the growth of your fruits if you damage their food source. You do not want to inflict any unnecessary trauma on your substrate.
If at all possible, get that tub outta your closet! Your tub needs as much FAE as it can get, which means keeping your tub at least 12" away from any walls on all sides. Also make sure your blanket doesn't obstruct any of the holes in your tub.
See if you can get your hands on a couple of 6500K CFLs to put in whatever space you dedicate to mushroom fruiting. I got mine at Lowe's, Sylvania brand. 6500K CFLs are made to mimic the sun; you'll also seem them referred to as "daylight" bulbs. Normal incandescent bulbs won't cut it, and I'd venture a guess that you may not be getting your shrooms adequate light w/them living (at least partially) in a closet.
Pics are always welcome! 
EDIT: Fruit that SOB!
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: masonwinston]
#18836479 - 09/13/13 11:11 AM (10 years, 4 months ago) |
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MORE GOOD NEWS! I have about 80% colonization or more on the top layer. I want to fruit but I feel if I wait till tomorrow I can get near 100%. I have a small touch light that is mostly a blue spectrum. Its just three small LED lights I believe. They appear very bright but puts off soft light. Do you think its necessary to get a different one? And I plan on fanning twice a day and misting when necessary. I hyave nio choice but to have it where it is at
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masonwinston
just a guy


Registered: 06/19/13
Posts: 57
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18836556 - 09/13/13 11:31 AM (10 years, 4 months ago) |
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Honestly man, I'm not sure what the blue spectrum is. 6500K CFL is what you want.
Sylvania bulbs from Lowe's
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: masonwinston]
#18838844 - 09/13/13 09:12 PM (10 years, 4 months ago) |
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Right on. I will grab some tomorrow. Started fruiting this morning. Have a fan blowing into the corner of the closet on low, not directly at the tub. All is looking well.
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18838858 - 09/13/13 09:15 PM (10 years, 4 months ago) |
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Honestly I am going to stay with my soft blue LED. I am reading up on how the light should be low in the red spectrum. I will look into it more. Plan on making a final decision tomorrow
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Skinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18839120 - 09/13/13 10:16 PM (10 years, 4 months ago) |
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My tip of the day is never think about the future as that can hold fear and never dwell on past mistakes. Focus on the amazement around you in each and every moment.
Remember - the future and the past are only hypotheses - all you are is a construct that has an awareness of being which exists in a singular moment of time which is NOW 
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: Skinty]
#18840615 - 09/14/13 10:29 AM (10 years, 4 months ago) |
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I kind of like to think of us as illusions of ourselves and we are but the manifestation of the perception of ourselves relayed from one person to the next to construct the world around us or what we think to be the world, but its just a manifestation of our collective consciousness that forms this reality which connects us to each other through what we see as the physical world since our human condition doesn't allow us to see the whole picture and realize we are one in a continuous perfect fashion. GOOD THOUGHT Skinty. Needed that wholesome brain food for though this morning. It was a good thing to ponder while taking my morning medication lol ON ANOTHER NOTE! Pretty much full colonization now. Hopefully by early next week I can report some pins.
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Skinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18843781 - 09/15/13 09:14 AM (10 years, 4 months ago) |
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Quote:
DaFun.Guy said: I kind of like to think of us as illusions of ourselves and we are but the manifestation of the perception of ourselves relayed from one person to the next to construct the world around us or what we think to be the world, but its just a manifestation of our collective consciousness that forms this reality which connects us to each other through what we see as the physical world since our human condition doesn't allow us to see the whole picture and realize we are one in a continuous perfect fashion. GOOD THOUGHT Skinty. Needed that wholesome brain food for though this morning. It was a good thing to ponder while taking my morning medication lol ON ANOTHER NOTE! Pretty much full colonization now. Hopefully by early next week I can report some pins.
Good work fun guy. We can only hypothesise and realise ey 
Good luck with your grow I just ate all mine I'm really full up. Time to hit the streets! 
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: Skinty]
#18848464 - 09/16/13 10:48 AM (10 years, 4 months ago) |
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Thank you and it seems that luck has not left me yet. MAN THIS MYC IS LOOKING PRETTY! Its very thick around the edges and is developing all these little nipple like bumps in the center. ONE QUESTION. The solid cake that is my substrate has seemed to of popped away from the one side of the tub leaving a small gap between tub and tub sized substrate cake, Will this cause anything unusual to happen? I did not use a trash bag inside my tub, I just used a black tub to block out light.
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DaFun.Guy
Stranger

Registered: 09/10/13
Posts: 22
Last seen: 10 years, 4 months
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Re: 10 days of colonizing PE's. Only 30% so far. Tips? [Re: DaFun.Guy]
#18861979 - 09/19/13 08:36 AM (10 years, 4 months ago) |
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Still no pins.... I am thinking this dinky LED light that I have above it is not putting enough light to go through the glass and layer of water on the inside of it and reach the substrate. The inside of the tub recieves almost no light even thought the light is directly above it. I bought a bigger LED lamp in the blue spectrum and now my substrate is illuminated. I would of bought the light you suggested Mason but I would of had to buy as lamp and figure out how to rig it in my closet. So instead I bought 5000k LED light that has alot of illumination but with soft white blue light
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