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Skinty
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Registered: 07/04/13
Posts: 1,150
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Any glaring issues with this syringe tek?
#18821982 - 09/10/13 03:41 AM (10 years, 5 months ago) |
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I've read the teks on the site for making spore syringes but somehow wasn't that satisfied with them so I made my own that seems easier and more sterile 
My plan is to use a very small jar with a self healing port filled with enough water to fill 4 syringes. I plan to sterilise this water in a PC 
In a glove box still air box I plan to quickly open the jar and scrape spores from a print into the sterile water and replace the lid. I reckon a quick swirl will distribute the spores 
After that stick a sterile syringe needle through the port, suck up spore solution and repeat till all syringes done...
This seems really quick and easy with minimal contam risk...any reason why this would not work?! 
Edited by Skinty (09/10/13 03:45 AM)
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Any glaring issues with this syringe tek? [Re: Skinty]
#18822041 - 09/10/13 04:46 AM (10 years, 5 months ago) |
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Not much experience myself, but I don't see any glaring issues with your plan...
What are you scraping the spores with? The reason I ask is because I used a disposable inoculation loop myself, and then I tried using the same loop for stirring... and yeah, the result was basically most of the spores lumping to the loop as i stirred. I had more luck with distributing the spores with the syringe (sucking in and releasing)..
Edit: Now that I think about it, are you sure that your syringes will be able to suck up the bottom remnants from the jar through the self healing port?
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Stromrider
This must be the place


Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
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Re: Any glaring issues with this syringe tek? [Re: Skinty]
#18822047 - 09/10/13 04:51 AM (10 years, 5 months ago) |
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I guess it would work as good as any other spore syringe tek outside of a lab environment.
That is what is so great about agar. It makes you more self sufficient. I mean no matter how sterile you are making the syringe there is no way your print is 100 percent sterile. With agar you can grow your spores out and transfer away from any contams. Then use a wedge to inoculate a grain jar or take a wedge to sterile water and make an Lc syringe to noc up cakes with.
Oh yeah and growing culture on agar is fun too!
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Any glaring issues with this syringe tek? [Re: Stromrider]
#18822051 - 09/10/13 04:54 AM (10 years, 5 months ago) |
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That a good point. OP has a PC, so agar is an option I guess...
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Any glaring issues with this syringe tek? [Re: Stromrider]
#18822091 - 09/10/13 05:40 AM (10 years, 5 months ago) |
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Quote:
psillyshroomer said: I guess it would work as good as any other spore syringe tek outside of a lab environment.
That is what is so great about agar. It makes you more self sufficient. I mean no matter how sterile you are making the syringe there is no way your print is 100 percent sterile. With agar you can grow your spores out and transfer away from any contams. Then use a wedge to inoculate a grain jar or take a wedge to sterile water and make an Lc syringe to noc up cakes with.
Oh yeah and growing culture on agar is fun too!
QFT
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Skinty
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Registered: 07/04/13
Posts: 1,150
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Re: Any glaring issues with this syringe tek? [Re: Pastywhyte]
#18822098 - 09/10/13 05:49 AM (10 years, 5 months ago) |
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Kjetterfaen - I plan on using just some metal wire flame sterilised. I won't need to use it to stir as can just swirl the spores / water solution in the jar. Also no, you a right, the needle doesn't reach the bottom of the jar but a bit of excess water and tilting the jar should work to fill the syringes. There will be a little bit of wasted spore solution but nothing to cry about 
psilly - next on the project list (after knocking up these syringes) is agar...I have not really looked into it too much as yet. My mind tells me it will be daunting although I'm sure it's not at the same time 
When i have a bit more time after Summer I will get going with that 
I was thinking of using a similar tek to do some GLC (yes - I know...agar!) - simply dump sterile water (in SAB) into the colonised grain jar, shake up and suck up through innoc port...simple!
Edited by Skinty (09/10/13 05:50 AM)
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Stromrider
This must be the place


Registered: 06/02/13
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Re: Any glaring issues with this syringe tek? [Re: Skinty]
#18822104 - 09/10/13 05:57 AM (10 years, 5 months ago) |
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Agar is not daunting. It is fun!
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Skinty
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Registered: 07/04/13
Posts: 1,150
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Re: Any glaring issues with this syringe tek? [Re: Stromrider]
#18822107 - 09/10/13 05:58 AM (10 years, 5 months ago) |
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Quote:
psillyshroomer said: Agar is not daunting. It is fun!
I don't have time for fun
No but really - I WILL get round to it eventually
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Stromrider
This must be the place


Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 22 hours, 16 minutes
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Re: Any glaring issues with this syringe tek? [Re: Skinty]
#18822129 - 09/10/13 06:10 AM (10 years, 5 months ago) |
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You better
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shroomdust
Psilovibin



Registered: 09/04/13
Posts: 114
Last seen: 9 years, 5 months
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Re: Any glaring issues with this syringe tek? [Re: Stromrider]
#18822352 - 09/10/13 08:01 AM (10 years, 5 months ago) |
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I think your tek will work. I used a similar tek that is very easy. I took a fresh water bottle and dumped 4/5 of the water out. Under my stove top fh, I unscrewed the cap and scraped my spores into the bottle and quickly screwed the lid back on. Next I shook the bottle up and wiped the outside of the bottle with alcohol where I would stick my needle through and proceed to draw up solution into my sterile syringes, wipe with iso and cap. Does anyone see any problems with this method of mine?
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Skinty
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Registered: 07/04/13
Posts: 1,150
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Re: Any glaring issues with this syringe tek? [Re: shroomdust]
#18822584 - 09/10/13 09:34 AM (10 years, 5 months ago) |
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Quote:
shroomdust said: I think your tek will work. I used a similar tek that is very easy. I took a fresh water bottle and dumped 4/5 of the water out. Under my stove top fh, I unscrewed the cap and scraped my spores into the bottle and quickly screwed the lid back on. Next I shook the bottle up and wiped the outside of the bottle with alcohol where I would stick my needle through and proceed to draw up solution into my sterile syringes, wipe with iso and cap. Does anyone see any problems with this method of mine?
Pretty similar ideas re: containing the spore solution in a sterile environment (well sterile before potential contam from the spores)...and you just reminded me - I am sterilizing tap water but I believe spring water is better?? I have a bottle but forgot :/
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shroomdust
Psilovibin



Registered: 09/04/13
Posts: 114
Last seen: 9 years, 5 months
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Re: Any glaring issues with this syringe tek? [Re: Skinty]
#18822649 - 09/10/13 09:54 AM (10 years, 5 months ago) |
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Yeah, spring water comes pre-sterilized inside the bottle so it is much less hassle than cleaning another vessel and boiling tap water. It also has minerals that I can only imagine would be beneficial to mycelium. No need for silicone either because you can just push the needle right through the plastic to draw up your solution. Of course it's only one time use but that's really all you need!
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Skinty
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Registered: 07/04/13
Posts: 1,150
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Re: Any glaring issues with this syringe tek? [Re: shroomdust]
#18822675 - 09/10/13 10:01 AM (10 years, 5 months ago) |
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Quote:
shroomdust said: Yeah, spring water comes pre-sterilized inside the bottle so it is much less hassle than cleaning another vessel and boiling tap water. It also has minerals that I can only imagine would be beneficial to mycelium. No need for silicone either because you can just push the needle right through the plastic to draw up your solution. Of course it's only one time use but that's really all you need!
I'd be a bit wary of the part where you are tipping water out though?? There would be a lot of air movement and therefore possible contam in contact with the water thats left surely?
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Any glaring issues with this syringe tek? [Re: shroomdust]
#18822686 - 09/10/13 10:04 AM (10 years, 5 months ago) |
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If anything bottled water tends to have less minerals, but in most cases, at the very least around here, bottled water is the same damn stuff as you get from the taps in the area where the tapping plant is located.
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Skinty
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Registered: 07/04/13
Posts: 1,150
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Re: Any glaring issues with this syringe tek? [Re: Kjetterfaen]
#18822706 - 09/10/13 10:09 AM (10 years, 5 months ago) |
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Quote:
Kjetterfaen said: If anything bottled water tends to have less minerals, but in most cases, at the very least around here, bottled water is the same damn stuff as you get from the taps in the area where the tapping plant is located.
Bottled water is bullshit - I hate people who buy a fresh bottle of water like 3 times a day or some shit a water bottle lasts me about 2 months refilling it...
Anyway - spring water SHOULD be from a natural spring but yr probably right
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Any glaring issues with this syringe tek? [Re: Skinty]
#18822720 - 09/10/13 10:13 AM (10 years, 5 months ago) |
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The thing is; our local water is ALSO from a natural spring... but that natural spring has a giant damn pipe and a factory in it. A natural spring just means no drilling and pumping was required to make the water surface... =/
Hell, if you have bad old pipes, or poor water in your area, then by all means buy bottled water. But I tend to agree with you regarding those who chug 2 litres of store-bought water a day... Anywhoooooo... done derailing.
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shroomdust
Psilovibin



Registered: 09/04/13
Posts: 114
Last seen: 9 years, 5 months
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Re: Any glaring issues with this syringe tek? [Re: Skinty]
#18822848 - 09/10/13 10:41 AM (10 years, 5 months ago) |
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I had the same thought afterward. I did do it under my stove top flow hood after lysoling everything but, maybe if I covered the mouth with a clean cloth and then poured it out, it would stop any airborne contams from entering? Or maybe just unscrewing the cap a little to let water out? Doing this in a SAB would prolly be better but, I still think it will work most if not all of the time if sanitary precautions are taken and you do it quickly. There's only one way to get the spores into a container..
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Any glaring issues with this syringe tek? [Re: shroomdust]
#18823203 - 09/10/13 12:15 PM (10 years, 5 months ago) |
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Quote:
shroomdust said: Under my stove top fh
Quote:
shroomdust said: I did do it under my stove top flow hood
I sure hope you aren't talking about the exhaust fan above your stove. The exhaust fan above your stove is NOT A FLOWHOOD! See ceiling fan tek: http://www.shroomery.org/forums/showflat.php/Number/18736785/fpart/1
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shroomdust
Psilovibin



Registered: 09/04/13
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Last seen: 9 years, 5 months
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Re: Any glaring issues with this syringe tek? [Re: Juiceh]
#18823654 - 09/10/13 02:27 PM (10 years, 5 months ago) |
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Well yeah, I know it isn't a true flow hood but, I don't think it's really comparable to the ceiling fan tek. Doing work underneath it seems safer because it sucks air/mold spores up away from my hands and out the front instead of just hitting the ceiling and getting pushed back down. I lysol'd the shit out of the whole thing too. I'm not saying it's right but, I've gotten away with it before..
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Any glaring issues with this syringe tek? [Re: shroomdust]
#18823786 - 09/10/13 03:05 PM (10 years, 5 months ago) |
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Read through that thread I linked to the end. As the exhaust fan pulls the air up, air from the surrounding area gets pulled in to replace that air and get pulled up again. This air is NOT HEPA filtered and is full of contaminants. The air flow is also not Laminar and smooth, it is turbulent swirling air. Swirling contaminants right into your work space. It is absolutely comparable to the ceiling fan tek. The difference between the two is the fan tek circulates shit and the stove top FH tek exhausts the air outside. However, the main problem with both is what I mentioned before, the swirling unclean air currents running right through your work space.
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