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N2ocean
Picture-er


Registered: 03/19/13
Posts: 42
Last seen: 8 years, 11 months
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a few quick questions, on grain, agar, etc?
#18821142 - 09/09/13 10:26 PM (10 years, 4 months ago) |
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Sorry I'm these can be found throughout the website but i'm done researching for the night and would like to ask a couple questions specific to my own experiences.
I'm having difficulty going from brf to grain, I've had successful colonization of agar plates, and of rye grain jars, but most are failures. Transfers are nightmares.
First, can you inoculate rye grain or wbs with a multi-spore syringe, or a syringe made from a print. I was under the impression that it needed to be from a living mycelial colony.
Second, my agar dishes that fail to colonize don't show any activity at all. They remain un-contaminated but don't show any myc growth. This has happened with MS syringes, spore print transfers. Any ideas, i close them with electrical tape, do they need a little bit of aeration (does parafilm provide that)?
Thank you so much if you are able to help!
-------------------- ---The mystics subjective experience of his identity with "the All" is the scientist's objective description of the ecological relationship of the organism/environment as a unified field - Alan Watts, The Joyous Cosmology An excellent, rather short read here----
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: a few quick questions, on grain, agar, etc? [Re: N2ocean] 1
#18821258 - 09/09/13 10:51 PM (10 years, 4 months ago) |
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Ofcourse you can inoculate grains with an MS syringe.
Colonizing dishes definitely need some gas exchange and yes that's exactly what parafilm does.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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N2ocean
Picture-er


Registered: 03/19/13
Posts: 42
Last seen: 8 years, 11 months
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Re: a few quick questions, on grain, agar, etc? [Re: mushmagic]
#18821265 - 09/09/13 10:56 PM (10 years, 4 months ago) |
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Thank you
-------------------- ---The mystics subjective experience of his identity with "the All" is the scientist's objective description of the ecological relationship of the organism/environment as a unified field - Alan Watts, The Joyous Cosmology An excellent, rather short read here----
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Dreaming Nomad
Psyborg



Registered: 01/15/13
Posts: 908
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Re: a few quick questions, on grain, agar, etc? [Re: N2ocean]
#18821330 - 09/09/13 11:15 PM (10 years, 4 months ago) |
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What do you mean by going from BRF to grain? And agar work, from what I gather, is more advanced and its probably best to stick with and master the basics first.
G2G transfers should be very easy and painless. What's causing your high rate of failure? Contamination? If so you just need to refine your sterile techniques.
1) Oh yeah! On my very first grow, I made my own syringe from a spore print and purified water and used it to noc up the jars. Now I just do G2G though because it's so much easier and faster.
2) I'm still learning myself, but the air may have something to do with stalled growth. My last batch of G2G jars all colonized very quickly and regularly, save one. This jar was going on 15 days and only 3-5% colonization, give or take, while all of the others hit 100% in 4-5 days. The jar only grew 3-4 tiny spots of super-dense, ultra slow irregular looking myc in all this time.
Finally I was like screw it, so I cracked the lid very slightly to give it some air. There mustve been a slight vacuum in the jar somehow, so air rushed in when I cracked it open. I closed it back right away and now it is exploding in regular, fast and rhizo growth.
Food for thought...
Feel free to hit me up with a msg if you want; I wouldn't mind sharing my experience
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They say curiosity killed the cat... Fortunately, I am not a cat.
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