Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineN2ocean
Picture-er
Male User Gallery

Registered: 03/19/13
Posts: 42
Last seen: 8 years, 11 months
a few quick questions, on grain, agar, etc?
    #18821142 - 09/09/13 10:26 PM (10 years, 4 months ago)

Sorry I'm these can be found throughout the website but i'm done researching for the night and would like to ask a couple questions specific to my own experiences.

I'm having difficulty going from brf to grain, I've had successful colonization of agar plates, and of rye grain jars, but most are failures.  Transfers are nightmares.

First, can you inoculate rye grain or wbs with a multi-spore syringe, or a syringe made from a print.  I was under the impression that it needed to be from a living mycelial colony.

Second, my agar dishes that fail to colonize don't show any activity at all.  They remain un-contaminated but don't show any myc growth.  This has happened with MS syringes, spore print transfers.  Any ideas, i close them with electrical tape, do they need a little bit of aeration (does parafilm provide that)?

Thank you so much if you are able to help!


--------------------
---The mystics subjective experience of his identity with "the All" is the scientist's objective description of the ecological relationship of the organism/environment as a unified field - Alan Watts, The Joyous Cosmology  An excellent, rather short read here----


Extras: Filter Print Post Top
Invisiblemushmagic
supporting radical habits
Male User Gallery


Registered: 03/21/12
Posts: 5,372
Loc: Candyland
Re: a few quick questions, on grain, agar, etc? [Re: N2ocean] * 1
    #18821258 - 09/09/13 10:51 PM (10 years, 4 months ago)

Ofcourse you can inoculate grains with an MS syringe.

Colonizing dishes definitely need some gas exchange and yes that's exactly what parafilm does.


--------------------


Trade list in journal (partially under construction; more to be added)


Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.


Extras: Filter Print Post Top
OfflineN2ocean
Picture-er
Male User Gallery

Registered: 03/19/13
Posts: 42
Last seen: 8 years, 11 months
Re: a few quick questions, on grain, agar, etc? [Re: mushmagic]
    #18821265 - 09/09/13 10:56 PM (10 years, 4 months ago)

Thank you :smile:


--------------------
---The mystics subjective experience of his identity with "the All" is the scientist's objective description of the ecological relationship of the organism/environment as a unified field - Alan Watts, The Joyous Cosmology  An excellent, rather short read here----


Extras: Filter Print Post Top
InvisibleDreaming Nomad
Psyborg
Male User Gallery


Registered: 01/15/13
Posts: 908
Re: a few quick questions, on grain, agar, etc? [Re: N2ocean]
    #18821330 - 09/09/13 11:15 PM (10 years, 4 months ago)

What do you mean by going from BRF to grain? And agar work, from what I gather, is more advanced and its probably best to stick with and master the basics first.

G2G transfers should be very easy and painless. What's causing your high rate of failure? Contamination? If so you just need to refine your sterile techniques.

1) Oh yeah! On my very first grow, I made my own syringe from a spore print and purified water and used it to noc up the jars. Now I just do G2G though because it's so much easier and faster.

2) I'm still learning myself, but the air may have something to do with stalled growth. My last batch of G2G jars all colonized very quickly and regularly, save one. This jar was going on 15 days and only 3-5% colonization, give or take, while all of the others hit 100% in 4-5 days. The jar only grew 3-4 tiny spots of super-dense, ultra slow irregular looking myc in all this time.

Finally I was like screw it, so I cracked the lid very slightly to give it some air. There mustve been a slight vacuum in the jar somehow, so air rushed in when I cracked it open. I closed it back right away and now it is exploding in regular, fast and rhizo growth.

Food for thought...

Feel free to hit me up with a msg if you want; I wouldn't mind sharing my experience :cool:


--------------------
They say curiosity killed the cat...
Fortunately, I am not a cat.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* New to the boards, and a quick question to start ! Anonymous 1,376 4 03/11/02 01:18 AM
by 2shrewmen
* Quick questions RE: Practicalities of Agar pouring Heruuka 1,592 5 10/10/02 02:22 AM
by Anno
* quick question for the millet heads ... keyeghost 562 1 09/06/02 05:26 PM
by mickey_rourke
* quick question. Legoulash 636 7 11/21/02 10:49 PM
by Skikid16
* Please help w/3 quick questions Chemical_Smile 1,151 9 10/13/01 01:16 PM
by CACA
* quick question...... Orbita6974 762 13 07/17/02 04:02 PM
by Orbita6974
* Quick question about quart/pint jars liquidplaya 2,543 18 09/20/01 06:03 PM
by darkfly
* quick question azazel 554 7 03/27/02 05:10 PM
by azazel

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
293 topic views. 26 members, 237 guests and 23 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.01 seconds on 14 queries.