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OfflineDeadPhan
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My first time making agar transfers. Pain in the ass.
    #18820280 - 09/09/13 07:20 PM (10 years, 4 months ago)

I'm not sure where I was going wrong.  I followed doc t's no pour agar tek and even made 17 jars from them instead of 15. I think my jars are too deep. When I tried to cut out a small square from a sector it was so hard to pick the piece up with the exacto knife.  Then, once I did, it was just as hard dropping the piece onto the clean plate I was transferring it to.  I even tried innoc loop a few times.  It was no easier or harder than using the exacto. To be honest, it was easier in certain respects but not to cut a nice sector. I had to use both in some cases just to get the damn agar off one or the other.  Is the thickness a factor in this situation?  Or do I just need to get my technique of cutting and lifting better?


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InvisibleSpitballJedi
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Re: My first time making agar transfers. Pain in the ass. [Re: DeadPhan]
    #18820300 - 09/09/13 07:24 PM (10 years, 4 months ago)

It takes a little practice. It seems slippery to pick up and then sticky to get off.

I use a stab and scoop motion to pick it up.

Jars were a PITA. petris are a little easier because they are shallow and you can get a good angle.

You really only need the agar to be deep enough to cover the bottom. The culture grows on top no matter how deep it is.


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InvisibleUhurungus
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Re: My first time making agar transfers. Pain in the ass. [Re: SpitballJedi]
    #18820709 - 09/09/13 08:40 PM (10 years, 4 months ago)

once you get your wedge on your knife, you can just stick it in the transfer jar/dish with a kind of stab and drag motion.  like you are cutting into the new agar only you have a wedge on the very tip.  it usually stays fine.  this works well even with larger peices as long as you do a good job flame sterilizing.


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OfflineDeadPhan
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Re: My first time making agar transfers. Pain in the ass. [Re: Uhurungus]
    #18822652 - 09/10/13 09:55 AM (10 years, 4 months ago)

Yea.  I was trying but those little buggers just wouldn't come off after sticking n most the time.  Does it matter if the mycelium surface is facing up down or sideways on new transfer plate or it will grow out the same regardless?

Also, when I start taking wedges and moving to grain will it matter if there is more uncolonized agar from it being deep on the wedge than mycelium that I'm transferring to the grain jar?


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Edited by DeadPhan (09/10/13 09:57 AM)


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InvisibleUhurungus
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Re: My first time making agar transfers. Pain in the ass. [Re: DeadPhan]
    #18823225 - 09/10/13 12:24 PM (10 years, 4 months ago)

does it matter?  if you want to split hairs... but we are talking 1 day difference or something stupid small.  could that mean the difference between contam or not?  sure... it could i suppose.  in my experience, if you have a sterilized knife, a nutritious medium, clean cultures, clean dishes, parafilm or the like to wrap and flowhood or sab, you wont have to worry about the actual transfering.

as long as your grain is sterilized properly and your dish/culture is clean it doesn't matter if you put an entire 100mm dish that is only 10% colonized in the grain.  everything is still clean, everything is still nutritious.  the mycelium will jump.  do this process 10 times and you'll look back and laugh at your former self. 

agar is easy.  it's just intimidating to beginners.

good luck


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InvisibleFrankHorrigan
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Re: My first time making agar transfers. Pain in the ass. [Re: Uhurungus]
    #18823241 - 09/10/13 12:28 PM (10 years, 4 months ago)

Practice practice practice.

No pour jars are difficult to work with due to the weird angle you have to reach in at. I recommend real, disposable petris.


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OfflineOregonMushys
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Re: My first time making agar transfers. Pain in the ass. [Re: FrankHorrigan]
    #18823263 - 09/10/13 12:36 PM (10 years, 4 months ago)

I was going to go ahead and start some petris but im worried because i dont have a flowhood. Am i trippin out a bit too much? Should i just go ahead and try agar in my SAB?


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InvisibleFrankHorrigan
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Re: My first time making agar transfers. Pain in the ass. [Re: OregonMushys]
    #18823271 - 09/10/13 12:39 PM (10 years, 4 months ago)

Quote:

OregonMushys said:
I was going to go ahead and start some petris but im worried because i dont have a flowhood. Am i trippin out a bit too much? Should i just go ahead and try agar in my SAB?




It's all I've ever done, for years.

Do it.

Agar is the best teacher for your sterile practice and contams on agar won't waste your time like it will on other media. Don't think of it as a foe to be conquered :lol:


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Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: My first time making agar transfers. Pain in the ass. [Re: OregonMushys]
    #18823272 - 09/10/13 12:39 PM (10 years, 4 months ago)

Yes, you should.


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OfflineDeadPhan
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Re: My first time making agar transfers. Pain in the ass. [Re: Pestile]
    #18824633 - 09/10/13 06:25 PM (10 years, 4 months ago)

Thanks for the advice guys.  I'm sure ill get better at it. I wasn't expecting it to be the least bit complicated so I was a lil taken aback. Anyway, also wondering, does it matter if the transfer piece just barely touches the new plate?  I felt inclined to push the transfer prices into the agar a little.  I'm guessing this is unnecessary though and the ,ycelium will grow just fine onto the new agar eve if it just falls off the scalpel onto the top?


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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InvisibleSpitballJedi
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Re: My first time making agar transfers. Pain in the ass. [Re: DeadPhan]
    #18824657 - 09/10/13 06:32 PM (10 years, 4 months ago)

Yep, it'll grow just fine.


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OfflinebEelzeBosS
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Re: My first time making agar transfers. Pain in the ass. [Re: SpitballJedi]
    #18824711 - 09/10/13 06:44 PM (10 years, 4 months ago)

No pour jars suck. I tried them because I messed up pouring my first set of petris. Didn't like them at all, so went back to petris and once you get the pour successful they are so much easier to work with. The only thing that I disagree with most on is I like glass petris.


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OfflineBlake_Shroom
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Re: My first time making agar transfers. Pain in the ass. [Re: bEelzeBosS]
    #18824726 - 09/10/13 06:48 PM (10 years, 4 months ago)

I might invest in glass petris, but I really do hate cleaning them.

Parafilm is another drawback to Petris, but a $40 roll will last you forever. I've gone through 2000 petris and there's barely a dent in the roll.


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OfflinebEelzeBosS
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Re: My first time making agar transfers. Pain in the ass. [Re: Blake_Shroom]
    #18824815 - 09/10/13 07:07 PM (10 years, 4 months ago)

I just use glad wrap, works fine and cheap


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OfflineDeadPhan
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Re: My first time making agar transfers. Pain in the ass. [Re: bEelzeBosS]
    #18827896 - 09/11/13 01:52 PM (10 years, 4 months ago)

K.  How long can I keep fully colonized agar dishes before I transfer to grain?  In the fridge obviously.


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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OfflineBlake_Shroom
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Re: My first time making agar transfers. Pain in the ass. [Re: DeadPhan]
    #18828045 - 09/11/13 02:30 PM (10 years, 4 months ago)

A while. Ive done it for a month. I wouldn't wait too long though. If u need long term storage then make some slants


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OfflineDeadPhan
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Re: My first time making agar transfers. Pain in the ass. [Re: Blake_Shroom]
    #18829413 - 09/11/13 07:12 PM (10 years, 4 months ago)

I plan too.  Are there any poor man slant teks out there.  Not sure how long it will be before I can afford some tubes. Considering agar is on its way, I guess I could.  Know of any good deals if not?


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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