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Offlinekrypto2000
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Would an ionizer create a positive pressure?
    #18818832 - 09/09/13 12:55 PM (10 years, 5 months ago)

I know they make the air electrostatic and thus cause things to stick to the surfaces which can aid in cleaning, which can be useful for mycology work. However I'm wondering if I should run it in my grow room. I currently don't have a way to cool the room and because I don't have screens on one of the windows (and the one that does is near a trash can), I'm trying to create a positive pressure in the room to for contaminant spores,dust, etc. out to minimize the necessecity to clean it on a regular basis. I would think an ionzer would create a positive pressure, but I don't really know. I don't want to run something in there that doesn't. I'm going to put a fan and an air purifyer in there which may be suitable enough, but the more electronics the more heat so I'm trying to keep it to a minimum.


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InvisiblebodhisattaMDiscordReddit
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Re: Would an ionizer create a positive pressure? [Re: krypto2000]
    #18818956 - 09/09/13 01:43 PM (10 years, 5 months ago)

I think you're over engineering this one. Fruiting isn't sterile(you could do it outside), and as far as a dirty room goes a good sterile technique is all you need. With a glove-box and sterile tek down you could work in a moldy room.


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Offlinekrypto2000
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Re: Would an ionizer create a positive pressure? [Re: bodhisatta]
    #18819162 - 09/09/13 02:45 PM (10 years, 5 months ago)

Well this is my bedroom/growroom and I also have a rabbit in the house (who I will no longer allow in the grow room). I'm currently cleaning it top to bottom, it's filled with dust and so is the house so I'm trying to make sure there's more pressure in this room to push the heat outwards and also prevent the rabbit hair, dust, and spores from accumulating in it. In the winter I'll just put a heater in there which will easily accomplish the same thing. I have practiced sterility and no matter what I cannot get an indoor grow that doesn't contaminate with trich. Outdoors I'm doing just fine, but indoors it's just not happening. Dust (and I have to assume spores) accumulate in my glove box even, it's rediculous. I'm pretty sure this place is built from trich spores w/maybe a little bit of wood and lead thrown in.


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InvisiblebodhisattaMDiscordReddit
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Re: Would an ionizer create a positive pressure? [Re: krypto2000]
    #18819193 - 09/09/13 02:54 PM (10 years, 5 months ago)

Quote:

krypto2000 said:
I cannot get an indoor grow that doesn't contaminate with trich.




It's not your room it's something you're doing. I use my glovebox in a moldy bathroom (since it's the only place I can keep draft free). Unless you're in a level 5 clean room there's going to be millions of spores on every surface around no matter how clean you are. There's 10's of factors that could contribute to your indoor trich problem but a clean room isn't going to help you since it will still have an incomprehensible number of rogue spores.

Surely my glove box is loaded with rogue spores but that's why we wipe them down with warm soapy water so that the spores stick to that. The box is not sterile at all but the spores don't fall on your work due to the still air which is a sterile technique.

I have a cat with long hair that sheds like mad and a wife with long hair but no problem is to be had here with trich except rarely after a 3-4th flush on a tray.


Edited by Trusted cuItivator (09/09/13 02:56 PM)


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Offlinekrypto2000
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Re: Would an ionizer create a positive pressure? [Re: bodhisatta]
    #18819224 - 09/09/13 03:02 PM (10 years, 5 months ago)

Hmm, well I'm continuing to try. Right now I've got some agar cultures going so at least I can start with a clean wedge. I have been using spores from trade or prints I've made myself for awhile, so it could be that is where the trich is coming from. There's just so many variables it's hard to pinpoint the culprit.


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Re: Would an ionizer create a positive pressure? [Re: krypto2000]
    #18819344 - 09/09/13 03:36 PM (10 years, 5 months ago)

agar is a smart choice. Spores are usually contaminated and could very well be the source of your woes.


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InvisibleSpitballJedi
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Re: Would an ionizer create a positive pressure? [Re: SpitballJedi]
    #18819366 - 09/09/13 03:43 PM (10 years, 5 months ago)

If you are having a lot of trouble, you may want to start a journal. Leave the journal open so we can post replies to help you. Load it with pics and details of your process. Journals and logs are standard scientific process.

If you already had a journal, I would just look at it and see if I notice anything  that might help. Because it's a journal, you can actually control who can post on it.

My journals have helped me help others help me. Just a thought


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InvisibleSpitballJedi
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Re: Would an ionizer create a positive pressure? [Re: krypto2000]
    #18819427 - 09/09/13 04:03 PM (10 years, 5 months ago)

You need a pretty big machine to create positive pressure in a room. The machine has to bring in air from outside the room, otherwise you would just be blowing the air around.

I have a big hepa filter i run in my room for a few hours before i to SAB work. I turn it off when I'm ready to start working.

It's probably not necessary because a SAB is really all I need. But it makes me feel better because I live in a dusty house.


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Offlinekrypto2000
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Re: Would an ionizer create a positive pressure? [Re: SpitballJedi]
    #18819451 - 09/09/13 04:07 PM (10 years, 5 months ago)

I do have a Hepa filter I could run in there as well, can't hurt. What's your procedure for doing that, just start it ~3 hours before SAB work and then turn it off a few minutes before? And I haven't made a journal on here before, is everyone allowed to see it by default? I'd love to start one if I could get constructive feedback from others along the way, that sounds like a great idea.


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InvisibleSpitballJedi
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Re: Would an ionizer create a positive pressure? [Re: krypto2000]
    #18819496 - 09/09/13 04:18 PM (10 years, 5 months ago)

I turn it on  2-3 hours before my SAB work and turn it off once I sit down and start work.

Everyone can see your journal by default, but you can change it easily. I make mine readable only by me until i get it done, then I edit it so others can read and reply.

If you open your journal and then click "access" at the top, you can even mange specifically who you want to be able to read and/or reply.


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Offlinekrypto2000
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Re: Would an ionizer create a positive pressure? [Re: SpitballJedi]
    #18819549 - 09/09/13 04:30 PM (10 years, 5 months ago)

How do you view others journals?


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InvisibleSpitballJedi
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Re: Would an ionizer create a positive pressure? [Re: krypto2000]
    #18819562 - 09/09/13 04:33 PM (10 years, 5 months ago)

You can click on the name and scroll down and click "journal" or some people, like me, have a little book icon under name and ratings and stuff.


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Offlinekrypto2000
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Re: Would an ionizer create a positive pressure? [Re: SpitballJedi]
    #18819591 - 09/09/13 04:40 PM (10 years, 5 months ago)

Ah, cool. Well I have a question or two for you on your SAB procedure if you don't mind. You don't mention putting things in the SAB before beginning work, do you just use your hands and put them in so it never touches the wet towel? I would think otherwise the towel would contaminate anything you set on it. Also do you use gloves or sleeves or anything or just wash your hands beforehand? I normally wipe mine with alcohol, wait for it to dry, and then put my things in there. I'll flame sterilize them before putting them in and right after inside the box (which likely creates a current, but what else am I supposed to do? Alcohol doesn't sterilize things.). Also I wash my hands/arms, then whipe with alcohol, and use gloves, but no sleeves, and then whipe the gloves with alcohol as well. I allow the alcohol to evaporate and then stick my hands in the box to begin my work.


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OfflinePussyFart
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Re: Would an ionizer create a positive pressure? [Re: krypto2000]
    #18819609 - 09/09/13 04:44 PM (10 years, 5 months ago)

The point of a SAB is to create a still air environment, nothing it it has to be sterile, or even sanitary for that matter.

Just let the air settle before going to work.

I use my gloved hands and an alcohol soaked paper towel to wipe everything down before it goes into the sab, then I close up the box, wait a minute or 2, and start working.


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InvisibleSpitballJedi
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Re: Would an ionizer create a positive pressure? [Re: PussyFart]
    #18819674 - 09/09/13 05:00 PM (10 years, 5 months ago)

My SAB journal is really just on how to make one. I'm actually working on a "How I use my SAB" journal.

But basically, the towel is a little damp, I wipe my jars and stuff while in the SAB. I wear tyvek sleeves and examination gloves with IPA.

Jars sit on the towel, petri dishes sit on a little wire rack. I don't wait for IPA to evaporate.

Like NH said, still air is the point. Having a little dampness helps trap things, but still air is really the main goal.

I use a light solution of soap and water for the inside and IPA for wiping gloved hands and jars and stuff.

My sleaves are currently the whole top half of a hooded tyvek suit, but I'm about to order some sleeves.

I do the whole shower, brush teath, mouthwash, and mask thing too.

Still air is the main goal, the other stuff is just extra precaution

Feel free to post comments or questions in my journal as well


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Edited by SpitballJedi (09/09/13 05:01 PM)


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Offlinekrypto2000
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Re: Would an ionizer create a positive pressure? [Re: SpitballJedi]
    #18819826 - 09/09/13 05:37 PM (10 years, 5 months ago)

Well what confuses me is for instance I use a box cutter blade to transfer my petris. So I can wipe the blade and then just set it on the bottom of the SAB? If things settle towards the bottom how is it that they don't settle all over the blade? Don't you want your tools to be sterile?


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OfflinePussyFart
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Re: Would an ionizer create a positive pressure? [Re: krypto2000]
    #18819835 - 09/09/13 05:39 PM (10 years, 5 months ago)

Yes you want your tools to be sterile, this is why we flame sterilize the tool outside of the box, and then bring it in while it is still hot.

But what are you wiping them with?

Because whatever it is it sure isn't sterile....chemicals sanitize, heat sterilizes.


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Re: Would an ionizer create a positive pressure? [Re: PussyFart]
    #18819982 - 09/09/13 06:20 PM (10 years, 5 months ago)

:whathesaid:

I have this wire rack from home depot or bed bath and beyond or somewhere.

I set petris on it for work. I flame sterilize a scalpel outside my SAB and stick in the edge of the agar to cool it.

I try not to set my scalpel down till I'm done using it. It takes a little dexterity tho.

This pic of the rack was taken outside the SAB so you can see it.



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Edited by SpitballJedi (09/09/13 06:23 PM)


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Re: Would an ionizer create a positive pressure? [Re: SpitballJedi]
    #18821449 - 09/09/13 11:56 PM (10 years, 5 months ago)

If you insist on a positive pressure room, depending on the size of crack under your doors, etc. Install a vet large squirrel fan to blow mass amounts of air into the room from an outside source.


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Offlinekrypto2000
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Re: Would an ionizer create a positive pressure? [Re: maddchef]
    #18822681 - 09/10/13 10:02 AM (10 years, 5 months ago)

Wouldn't that create a lot of turbulence and be bad for the mushies since it'd be pulling in spores from outside?


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