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OfflineStromriderM
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18815755 - 09/08/13 04:02 PM (10 years, 5 months ago)

Definitely use a liner next time and definitely don't make an lc. Lc's are too sketchy man. Get you some agar and petri dishes.

Hell you'd be better off taking tissue straight to grain than you would lc


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OfflineBlackieChan
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Re: My First Grow [Monotub Log] [Re: Stromrider]
    #18815797 - 09/08/13 04:15 PM (10 years, 5 months ago)

Quote:

psillyshroomer said:
Definitely use a liner next time and definitely don't make an lc. Lc's are too sketchy man. Get you some agar and petri dishes.

Hell you'd be better off taking tissue straight to grain than you would lc




I was thinking I'd make one of those master cultures with karo or honey, and make some working cultures from them. I like using syringes and the idea of innoculating 40 jars at once sounds awesome to me. What is it about them that you don't like? I haven't heard anything negative yet.


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OfflineStromriderM
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18815816 - 09/08/13 04:20 PM (10 years, 5 months ago)

Well the main thing is that if an Lc is contamed you can not see it.

Also all you really need is one coloinzed grain jar then you can use it to make 10 to 15 more jars doing g2g transfers. It is a super fast way to increase your stock culture. Even faster than inoculating with a lc


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OfflineBlackieChan
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Re: My First Grow [Monotub Log] [Re: Stromrider]
    #18815842 - 09/08/13 04:31 PM (10 years, 5 months ago)

Quote:

psillyshroomer said:
Well the main thing is that if an Lc is contamed you can not see it.




I thought if you used all clear ingredients you could see it? I assume the main contaminates are green and black? I also hate ordering online and paying shipping, not mention I'm very careful (paranoid) and buy all my supplies in cash from separate stores, so I am wanting to avoid buying petri dishes and dextrose/agar online. Any suggestions where to buy locally?
Quote:

psillyshroomer said:
Also all you really need is one coloinzed grain jar then you can use it to make 10 to 15 more jars doing g2g transfers. It is a super fast way to increase your stock culture. Even faster than inoculating with a lc






yeah that's what I did with this grow. I took 4 jars into 40. They colonized pretty fast, not sure on time because I'm too busy to check every day. Which is why the set and forget strategy was exactly what I needed. I just remember checking and about half the jars were done and the other half were about 80%.

One jar did get trich in a small spot, i shook it and it recolonized in a day but i destroyed it anyway to be safe. The last thing i wanted was to make a tub, have it grow a bunch of trich while I was away, and cover my house in spores.

Now I'm wondering if I should take my 4 remaining fully colonized jars left over and make another 40? Or if I want to wait and use a clone. I'm not too familiar with strain isolation but I think the community is pretty in unison about the benefits so I want to try it out.


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OfflineStromriderM
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18815861 - 09/08/13 04:39 PM (10 years, 5 months ago)

Taking tissue straight to grain can work but you have to have excellent sterile procedure. There is a mushroom cultivation shop near where I live in Asheville NC where I buy all my agar supplies. He has a website and can ship you stuff discretely. You can also call him and do it over the phone


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OfflineBlackieChan
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Re: My First Grow [Monotub Log] [Re: Stromrider]
    #18815875 - 09/08/13 04:43 PM (10 years, 5 months ago)

Quote:

psillyshroomer said:
Taking tissue straight to grain can work but you have to have excellent sterile procedure. There is a mushroom cultivation shop near where I live in Asheville NC where I buy all my agar supplies. He has a website and can ship you stuff discretely. You can also call him and do it over the phone




what's his website?


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OfflineStromriderM
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18815886 - 09/08/13 04:46 PM (10 years, 5 months ago)

His website is fungiasheville. Com

I think I also forgot to mention that working with your culture on agar is fun as hell


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OfflineMidnight Cyclone
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Re: My First Grow [Monotub Log] [Re: Stromrider]
    #18817000 - 09/08/13 10:03 PM (10 years, 5 months ago)

Yeah, you learn pretty quickly that side pins can be a pain and IMO are generally best to avoid.

Quote:

With my next grow I'm going to use liners.




:thumbup:

Quote:

I will be taking a live tissue sample from a large mushroom and making some liquid culture.




Just be careful with LCs. You have to test them to make sure they're not contaminated. Test them on a small number of jars as to eliminate the potential of ruining an entire batch of jars.

You should look into agar man. Agar is best practice and will give you better results grow to grow if you can learn to isolate and whatnot. I say you should learn agar for the sole purpose of knowing you have a clean set of inoculant. Then you don't have to worry about contams from any sort of syringe or LC.

I'm still not into agar, and I do use LCs. Just saying you must be pretty sterile while using an LC and also making sure you're on the right track to success with agar to look forward to.

Keep up the fresh air and the updates :thumbup:


Edited by Midnight Cyclone (09/08/13 10:07 PM)


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OfflineBlackieChan
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Re: My First Grow [Monotub Log] [Re: Midnight Cyclone]
    #18817093 - 09/08/13 10:41 PM (10 years, 5 months ago)

This came out of no where today!

I'm so sad!


What do I do?


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OfflineMidnight Cyclone
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18817105 - 09/08/13 10:44 PM (10 years, 5 months ago)

Definitely trich.

So contamination was the culprit.

Quote:

What do I do?




Quarantine that tub in hopes of not spreading trich to your others.


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OfflineBlackieChan
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Re: My First Grow [Monotub Log] [Re: Midnight Cyclone]
    #18817226 - 09/08/13 11:14 PM (10 years, 5 months ago)

I harvested the tub and threw it out. I actually dumped the cake in my back yard just to see what will happen to it. I put the cubes I harvested in my dehydrator.


Now a bunch of other tubs are pinning before full colonization. If my whole crop is contaminated that is really going to suck. Idk what I did wrong. I followed the damion coir tek exactly. It might have been that one of my cats pawed the polyfil out of a hole in the tub. I didn't notice trich till after that. who knows.


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Offlinedruranium
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18817358 - 09/08/13 11:43 PM (10 years, 5 months ago)

I'm surprised my cat hasn't attacked the polyfill 'toys' on my tub.  Man, that sucks.  Well at least you got a little bit of fruit out of it and you live to try again another day. 
I used Frank's tek to pasteurize the coir/verm and knock on wood no contam yet.  Had a small 1st flush and just letting it rest before dunking and seeing what happens on flush 2


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OfflineStromriderM
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Re: My First Grow [Monotub Log] [Re: druranium]
    #18817790 - 09/09/13 04:16 AM (10 years, 5 months ago)

You guys it doesn't matter if the polyfill falls out of the holes you the cat paws it out or whatever. Properly pasteurized substrate is contam resistant. When mine starts to pin I leave one of the holes open. I also take the lid off daily to peek.

Blackie just water that sub that you threw in the back yard daily and you should still get some nice fruits off of it


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InvisiblePastywhyteMDiscord
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18817822 - 09/09/13 04:45 AM (10 years, 5 months ago)

Quote:

BlackieChan said:
I started with a spore print of berma. Transfered in open air to a syringe. I inoculated 4 quart jars of organic rye berries. Once fully colonized I g2g the 4 into 40 jars in a glovebox I made.





Open air = fail.

Why would you make your syringe in open air if you had a SAB for G2G? Anyway be sure to use your SAB for that kind of work and you will have better success. Also agar is the shit, get it going ASAP :super:


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OfflineStromriderM
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Re: My First Grow [Monotub Log] [Re: Pastywhyte]
    #18817824 - 09/09/13 04:47 AM (10 years, 5 months ago)

:whathesaid:


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OfflineBlackieChan
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Re: My First Grow [Monotub Log] [Re: Pastywhyte]
    #18817856 - 09/09/13 05:23 AM (10 years, 5 months ago)

Quote:

Pastywhyte said:

Open air = fail.

Why would you make your syringe in open air if you had a SAB for G2G? Anyway be sure to use your SAB for that kind of work and you will have better success. Also agar is the shit, get it going ASAP :super:




Hadn't made the glove box yet.


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OfflineStromriderM
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18817884 - 09/09/13 05:47 AM (10 years, 5 months ago)

I don't like your sig blackie. :tongue:


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Offlinedruranium
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Re: My First Grow [Monotub Log] [Re: Stromrider]
    #18819065 - 09/09/13 02:15 PM (10 years, 5 months ago)

Ha ha, yeah the NSA probably has a special algorithm to seek out drug pics and scan the exif for coordinates.  It's already come out that they are sharing info with the DEA. 
Man, all this stuff that used to be ridiculed as 'paranoid tinfoil hat talk' is reality now. 

This is a good free mac app that strips exif:
Imageoptim

Good luck w/ your outdoor bed blackie!


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OfflineBlackieChan
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Re: My First Grow [Monotub Log] [Re: druranium]
    #18819370 - 09/09/13 03:46 PM (10 years, 5 months ago)

Quote:

druranium said:
Ha ha, yeah the NSA probably has a special algorithm to seek out drug pics and scan the exif for coordinates.  It's already come out that they are sharing info with the DEA. 
Man, all this stuff that used to be ridiculed as 'paranoid tinfoil hat talk' is reality now. 

This is a good free mac app that strips exif:
Imageoptim

Good luck w/ your outdoor bed blackie!




another good mac app is opening the picture in preview and taking a screen shot. haha.


and an update, I currently have two tubs in fruiting with an oscillating fan blowing on them.


Where do you think I went wrong making the substrate? The jars colonized perfectly and I tested every single jar by shaking and letting it recolonizing before spawning with it because I was in no hurry.

When preparing the substrate I put a brick of coir into a 5 gallon bucket, added 2 quarts of vermiculite, put the lid on, boiled 4.5 quarts of water, dumped in the bucket and snapped the lid on tight. After 45 minutes I stirred the bucket up and added a digital thermometer on a long wire that I could leave the read-out on top of the bucket. I waited till between 80-90 degrees. I then wiped my tub down with IPA and dumped 3/4 of the bucket, emptied 5 spawn jars into it, mixed it in well with no gloves, even it out, dumped the rest of the substrate, evened it out, and put the lid on. I don't understand what I did wrong. 


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Edited by BlackieChan (09/09/13 03:51 PM)


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OfflinePussyFart
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Re: My First Grow [Monotub Log] [Re: BlackieChan]
    #18819383 - 09/09/13 03:49 PM (10 years, 5 months ago)

Quote:

BlackieChan said:
another good mac app is opening the picture in preview and taking a screen shot. haha.



That is not only for mac, there is a PrtScrn button on every keyboard that does the same thing.


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