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Offlinesocraticd
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Socraticd's experiments - a.k.a, a newbie's grow log
    #18816966 - 09/08/13 09:54 PM (10 years, 5 months ago)

So, I've been lurking for awhile and have posted a few questions as I start to dip my toes in to grain spawn with WBS and such.  Have done a small grow on pf cakes with a lions mane LC syringe I ordered, and grew out a pre-made blue oyster log.

Three of the PF lion's mane cakes in a PMP I constructed:


The blue oyster log I grew out in June:


I took prints off both of those grows, before I really started learning that with edibles, it'll be a LOT better for me to be working with clones and isolates on agar.  But, taking the prints and making a couple spore syringes was good practice anyway, and I'll experiment with the MS inoculations and maybe I'll get lucky and get a good strain to work with.


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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18817012 - 09/08/13 10:08 PM (10 years, 5 months ago)

:asianofapproval:


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18817015 - 09/08/13 10:11 PM (10 years, 5 months ago)

There were actually 5 cakes made with the LC of Lion's Mane, two of them I attempted to spawn into probably 5 lbs of unsupplemented sawdust from hardwood fuel pellets a'la Lipa's pasteurization tek.  There's a post somewhere about being curious of very very slow lion's mane growth, which I now suspect was likely a combination of too wet sawdust and not nearly high enough spawn ratio.  I've let that bag sit for quite some time and believe it's been colonizing for about 6 weeks now.  It's actually bouncing back, albeit slowly, so I'll grab a picture of it tomorrow.

Fast forward a bit and I finally got around to inoculating some WBS jars with the spores referenced from the two grows above.  The blue oyster prints were VERY weak, as I waited too long to put a couple fruits on foil and got very light prints, almost not detectable.  I made a single syringe out of the two prints anyway, not 100% sure I got ANY spores in it.  The Lion's Mane print made three dark (well, cloudy, since LM spores are white) syringes. 

Inoculated 7 WBS jars (which I posted about being too wet, a short while back) on 8/10.  2 of them with the seriously weak blue oyster syringe, 5cc each (that certainly didn't help my wet WBS issue...) and 5 with one of the Lion's Mane syringes, 2cc each.

I didn't have much hope for the oysters, but 4 weeks later I had this (after a shake about a week ago when they were looking pretty well colonized already).



The LM jars have been looking slow, but I believe are colonized reasonably well and hopefully will thicken up soon?  The one on the far right has been shaken up to see if this helps strengthen the remaining colonization.  Still learning a lot, here.



The one other thing I'm concerned about, is the blackening of a decent amount of the bird seed in most of the jars.  I believe this is where some of the excess water was found.  Looks like the bird seed discolored.  Possibly bacterial?  The mycelium isn't really messing with it too much, so my guess is the excess moisture became a bacterial wonderland and now there's a battle in these jars?



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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18817037 - 09/08/13 10:17 PM (10 years, 5 months ago)

Which more or less brings me to today.  After essentially lurking and digesting information in here NON STOP.  Edibles forum, advanced, cultivation, etc etc etc... 

I've acquired a couple bales of straw from Lowes to try my hand with straw buckets/bags for the oysters, and spawned those two oyster jars above to this bucket.  3 gallon bucket with probably 2.75 gallons of straw (underestimated how much I needed when I was cutting it up).  "pasteurized"/cleaned with hot water and bleach (4 cups bleach to ten gallons hot water... probably overkill on the bleach) for 4.5 hours or so in a cooler.  Mixed in the grain spawn after breaking it up really well and stuffed it into a garbage bag in the bucket that I put 12 1.5" holes in.  I'll let this colonize for two weeks. and then cut slits in the bag where the holes are.  Should I put any holes in the bottom of the bag in case there's standing water down there?  or just leave it completely sealed until 2 weeks from now?  poke small holes in the bag now?  or wait?



The little baggie next to it was the leftovers of spawn and straw that was stuck to the sides of the cooler, and the stuff that was on my kitchen floor when I was done.  I figured I'd throw it in a grocery bag and see if it does anything.  Worst case, it ends up in the trash just like it would have if I had cleaned up normally...


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18817061 - 09/08/13 10:25 PM (10 years, 5 months ago)

Last one for tonight.  I also grabbed 4 LC syringes from a vendor, to expand my experiments.  King Oyster, Pink Oyster, Blue Oyster (assuming my experiment with the print from the previous grow would fail), and Ghost Fungus. 

As you can see I upgraded my jar lids to RTV and tyvek (LOVE those lids!).  Found out my capacity on the 23qt Presto is 10 quart jars.  Inoculated 4 jars with pink oyster, 3 with blue oyster, 2 with king oyster, and 1 ghost fungus.  I'll be keeping up with this thread as things progress and as I have more questions, which are sure to come up.



Next up, agar!  ;-)


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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18817383 - 09/08/13 11:51 PM (10 years, 5 months ago)

:thumbup: Nice


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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: skullphuxxx]
    #18818104 - 09/09/13 08:03 AM (10 years, 5 months ago)

You're on a roll mate!  Nice to see the progress


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: forrest]
    #18850792 - 09/16/13 07:16 PM (10 years, 5 months ago)

So this weekend I busted my agar cherry.  First time through so it was a little awkward at first.  I think the fact that I used a growler for my agar vessel didn't help in that, as it was a little on the heavy side and tough to maneuver in my SAB.




This is the workspace all set up.  The growler contains a mixture of 10g malt extract, 9g agar, 500ml water.  Pressure cooked at 15psi for 20 minutes and allowed to cool until I could handle it with no real heat concerns.  However, due to the amount of condensation on the plates, maybe I should let it cool a bit more next time?  Or is the condensation really nothing to worry about?


The work area after I'd poured about 17 plates (some of them were a little thick, so I can likely stretch the 500ml out to a full sleeve's worth of 25 plates next time. Though maybe I'll make 600ml to be safe?)



The plates cooling





I then inoculated 3 - 4 plates each with a couple drops of the liquid culture syringes mentioned from the post with the WBS the jars above.  Those jars have shown ZERO growth in a week, which is very very weird.  Not a single one of them has any signs of growth.  Here are the finished plates.




The WBS jars with no growth are puzzling me.  So, to see if it was just that batch of jars, I made 8 pf tek jars and inoculated 2 jars with about 1-2 cc of liquid culture, and then the above mentioned plates.  I'm trying to figure out if it is the syringes that were bad, or if I somehow screwed up the whole batch of 10 WBS jars?  The jars weren't completely room temp, but they weren't even remotely something i would condsier "hot" when i inoculated.  I'd have rated them at lukewarm.  They got 2cc each of LC from the syringes, which has only been out of the fridge for ten minutes.  Maybe that is a no-no?  I would think at worst that would only have stunted them by a couple days?  Thoughts?


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18850803 - 09/16/13 07:17 PM (10 years, 5 months ago)

I guess between the pf jars and the agar plates, I'm hoping to get an answer on whether the syringes were bunk or if i messed up the WBS jars.  I left the syringes at room temps for three days prior to inoculating the pf jars and plates, to try to eliminate the "fridge" variable...  :rolleyes:


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Offlinegeneralsherman55
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18851137 - 09/16/13 08:37 PM (10 years, 5 months ago)

i love this thread! i spent way too much time reading threads here today looking for a cheap way to clone my chicken of the woods sample (which might give me mild poisoning)

found a tutorial on how to make a still air box

but I'm still sort of unsure where to get the rest of the materials

agar (someone suggested from an asian market)

pretri dishes

and then im wondering if i should go to a thrift store to look for a pressure cooker

and then where to get needles.

im all over the place right now with possibilites but im sort of unsure how to proceed especially since I'm broke.  Ideally id like to get the clone started while my mushrooms are still good. (theyre chilling in the vegetable crisper in the fridge right now)


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OfflineForresterM
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18852012 - 09/17/13 12:09 AM (10 years, 5 months ago)

Quote:

socraticd said:
There were actually 5 cakes made with the LC of Lion's Mane, two of them I attempted to spawn into probably 5 lbs of unsupplemented sawdust from hardwood fuel pellets a'la Lipa's pasteurization tek.  There's a post somewhere about being curious of very very slow lion's mane growth, which I now suspect was likely a combination of too wet sawdust and not nearly high enough spawn ratio.  I've let that bag sit for quite some time and believe it's been colonizing for about 6 weeks now.  It's actually bouncing back, albeit slowly, so I'll grab a picture of it tomorrow.




I think you're probably right on the the substrate too wet thing.  Especially if you followed Lipa's tek, which calls for more water than pellets.  As I'm currently learning, Lion's mane hates too wet of a substrate.  Lipa's tek calls for what 6:7 pellet:water ratio?  I'm already to a 1:1 ratio and that's even been too wet lately.  Of course pellets can vary...


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: Forrester]
    #18853560 - 09/17/13 01:34 PM (10 years, 5 months ago)

Quote:

Forrester said:
I think you're probably right on the the substrate too wet thing.  Especially if you followed Lipa's tek, which calls for more water than pellets.  As I'm currently learning, Lion's mane hates too wet of a substrate.  Lipa's tek calls for what 6:7 pellet:water ratio?  I'm already to a 1:1 ratio and that's even been too wet lately.  Of course pellets can vary...





Yeah, its actually progressing and co solidating a bit now, just has been EXTREMELY slow.  Probably 2 months since spawning to sawdust now.  Combination of too much moisture and too low a spawn ratio.  Once the wbs jars fully colonize I'll do another couple of sawdust bags with a little less water and more spawn, and imagine it'll go more smoothly. 


Anyone have any feedback on my oyster straw bucket. I'm 9 days put after spawning and wondering of I should poke holes in the bag at the bucket holes or not.  Right now its more or less completely sealed up, with no holes in the bag and the top tied off.  Thoughts?


(I wish this forum got half as much traffic as cultivation does.. )


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: Forrester]
    #18853568 - 09/17/13 01:35 PM (10 years, 5 months ago)

Quote:

generalsherman55 said:
i love this thread! i spent way too much time reading threads here today looking for a cheap way to clone my chicken of the woods sample (which might give me mild poisoning)

found a tutorial on how to make a still air box

but I'm still sort of unsure where to get the rest of the materials

agar (someone suggested from an asian market)

pretri dishes

and then im wondering if i should go to a thrift store to look for a pressure cooker

and then where to get needles.

im all over the place right now with possibilites but im sort of unsure how to proceed especially since I'm broke.  Ideally id like to get the clone started while my mushrooms are still good. (theyre chilling in the vegetable crisper in the fridge right now)





Thanks!  Glad you like it.  I'm hoping that other newbies followong along with me can learn from my questions and mistakes.


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Offlinegeneralsherman55
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18854128 - 09/17/13 03:43 PM (10 years, 5 months ago)

Quote:

socraticd said:
Quote:

generalsherman55 said:
i love this thread! i spent way too much time reading threads here today looking for a cheap way to clone my chicken of the woods sample (which might give me mild poisoning)

found a tutorial on how to make a still air box

but I'm still sort of unsure where to get the rest of the materials

agar (someone suggested from an asian market)

pretri dishes

and then im wondering if i should go to a thrift store to look for a pressure cooker

and then where to get needles.

im all over the place right now with possibilites but im sort of unsure how to proceed especially since I'm broke.  Ideally id like to get the clone started while my mushrooms are still good. (theyre chilling in the vegetable crisper in the fridge right now)





Thanks!  Glad you like it.  I'm hoping that other newbies followong along with me can learn from my questions and mistakes.





yeah i have been hunting for a while now, id still say i am an amateur identifier but hunting is a really big passion of mine


BUT


now i really want to get into cultivation because growing shit in my backyard and producing bulk mushrooms sounds fanbleedingtastic


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OfflineForresterM
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #18855950 - 09/17/13 10:46 PM (10 years, 5 months ago)

Quote:

socraticd said:
Anyone have any feedback on my oyster straw bucket. I'm 9 days put after spawning and wondering of I should poke holes in the bag at the bucket holes or not.  Right now its more or less completely sealed up, with no holes in the bag and the top tied off.  Thoughts?





Definitely cut x's / poke holes now.  You should do this from the beginning with oysters.  They need gas exchange at the very least, for colonization.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: Forrester]
    #18871640 - 09/21/13 11:11 AM (10 years, 5 months ago)

Well, the grocery store baggie with the leftover straw/spawn from my oyster bucket experiment was contam'd with blue/green stuff amongst the white clumps of what I imagine were mycelium...  So, that got tossed.

The bucket was left with no holes until a few days ago, and when I cut slits in the holes i didn't really see any signs of colonization, and it smells kinda...  fermented.  I'm thinking it's possible that the substrate was too wet (I squeezed as much liquid as I could out of the straw, but who knows...) and that combined with the lack of any GE whatsoever stalled things?  I'll give it a couple more weeks to see if anything happens, and then dissect it if it doesn't seem to be producing anything  I figure that's my best shot at learning from it.

The agar plates from last week did pretty well. Only one or two possible contams out of 16 plates.  No growth from any of the LCs I was testing, however.  Fortunately, the vendor is happy to send me replacements, so I should be able to get those all going again in the next week.

My only complaint with last week's plates was a LOT of condensation on them.  It doesn't seem to have increased contamination or anything, just makes them hard to work with and see into. 

Did 14 more plates this week. I made 600ml of agar and only got 14 plates out of it.  Was confused as hell since i got 16 out of 500ml last week, but realized i poured them a LOT thicker than I thought, once I was done.  Oh well, it's all a journey, right?



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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19060669 - 10/30/13 10:06 PM (10 years, 3 months ago)

Have been ignoring this thread, unfortunately, even thought I've made some significant improvements in my technique and equipment. 

Picked up a 56lb bag of rye since my results with WBS were inconsistent, and built a new glovebox and have been doing experiments with agar lately.  Trying to learn how to pour more consistent plates, eliminate condensation, and find out through experimentation what my contamination rates are like when i do various things with the plates once poured:

- leave them un-parafilmed in the box for upwards of a week or two to evap condensation, then inoculate and observe condensation
- pour half a sleeve and tie off the other half of the sleeve, then pour the other half a sleeve a week or so later
- take wedges from one colonized or partially colonized plate and expand to several plates

seems as if i can leave the plates for any period of time, poured and not parafilm'd in my sealed SAB without any additional risk of contamination, from the 30 or 40 plates i've tested this with.  maybe 1 contam'd plate out of approximately that many, which I'll gladly accept compared to having tons of condensation on the plates between when i pour them and when i use them.

the half-sleeve experiment went well, also.  out of 7 or 8 plates only  1 contam, which could have been due to leaving them in the SAB without parafilm for two weeks, as well.  Either way, good news for me to be able to pour half a stack for whatever reason, if I'm careful about how i remove them from the sleeve.

first attempt to expand mycelium was a contaminant disaster and i had essentially a 100% contam rate on 10 plates or so being expanded from 3 starter plates.  I attribute this to having to constantly pull my arms out of the SAB to flame sterilize the exacto "scalpel" between cutting and transferring wedges.  I've since tried thoroughly alcohol wiping the blade between transfers and it seems as if my contam rates there are significantly lower, but this latest batch of transfers from a fully colonized reishi plate to 10 new plates will give me more info on how that worked out.

Some lions mane grain spawn is WAY overdue to be mixed into a sawdust bag, and a couple lions mane sawdust bags are PROBABLY ready to start fruiting, which means i need to get off my ass and get my greenhouse set up in the basement.  One lions mane sawdust block is WAY WAY past due to be fruited and is now thick mycelium everywhere aand has turned pink in a decent number of spots... 

Like I said, pics within the next day or two, since all threads are worthless without those, huh?

Hopefully some new syringes on the way to get working with, since I appear to have been screwed over by a non-sponsor vendor on king oyster, blue oyster, pink oyster, and ghost fungus...  Ordered king oyster, enoki, and pearl oyster from The Spore Depot today, so I imagine I'll get those quickly and will be rockin' and rollin' with some new stuff soon.  :-)


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Offlinekushrooms
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19060776 - 10/30/13 10:24 PM (10 years, 3 months ago)

Can't wait to see your pictures. Everything looks great, those Oyster you grew look delicious!

I know I've had contam issues with agar and glove box, I just have to be as careful as possible.

I use a 32oz beer bottle to pour the agar and try to do it quickly and as soon as it hardens I wrap them completely shut. Then stack them. I was surprised when I checked on them and I didn't find contam but the agar had totally evaporated from the Y-Plates lol I didn't know they could do that.


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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19061092 - 10/30/13 11:21 PM (10 years, 3 months ago)

This is a weird thought i've had but since i no longer use a glove box i havent tried it.

My idea is to put a small slit in the side of the gb, just big enough to slip the tip of your scalpel through, then on the other side put your lantern which you can then flame sterilize without moving your arms in and out of the box.

Might be counter productive because at that point you'd have a flame moving air around a small hole in the still air box.. :shrug: but i would much rather flame sterilize than trust alcohol before doing agar work.


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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: kushrooms]
    #19061129 - 10/30/13 11:29 PM (10 years, 3 months ago)

Ugh, condensation issues... having the same problem lately.  I really, really, really wish I had a flow hood.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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