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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
trying agar again advice appreciated :)
    #18813709 - 09/08/13 01:08 AM (10 years, 5 months ago)

well ive tried agar a couple of times and it didn't work out so well:shrug:... but ive decided to try again since i have a bad ass flow hood now :laugh:. i figured this time i would try pouring into some pre sterilized plates as well as trying again at no pour. for the no pour i mixed 200ml of water with 2g of elme and 2g agar and placed into various small jars then pc'd for 45 min and let cool. prepping to pour the agar i mixed 500ml water with 10g elme and 9g agar into an empty liquor bottle and pc'd for 15 min with lid loose on the bottle but no filter in lid. after about 30-45 min i removed bottle from pc and poured a total of 11 petri dishes with it but i think i filled them a little high...:shrug: lol i then wrapped them in micro pore tape...that was a bad idea lol next time i will let them cool in front of the flow hood and then wrap them. :smile: but other than that is there anything i maybe should do or try differently the next time i make a batch of agar? and which culture should i attempt to isolate for my newly made agar PE, PB, B+ GT, Orissa, or AA+? i'll log the iso for advice too :smile: i've started a new grow with PE, PB, and AA+. AA+ deffinately looks to be doing the best of the ms from each. :vaped:


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflineHofmann1943
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18813984 - 09/08/13 03:27 AM (10 years, 5 months ago)

hahahahah yea i know how frustrated agars can be.
Here is a couple of tips for you as a friend.

First thing first

Wash all plates well then put them in plastic bag for sterilization of some bigger jar the point is while sterilizing them non of boiling water should break in them only steam.

When making agar use some kind of Jar or bottle that wont leak all over the place when pouring agar in to petri dishes. Yes make the lid loose.
4% remember that it means on 96 ml of distillate water 4g of dry matter

2g malt
2g agar agar

measure your plates how much water is enough to fill it to the middle. Then you will know how much of agar you will need for how much dishes.

Use dishes not bigger than 10 cm in diameter but use deeper dishes 1.5 - 2 cm
don't fill them more than a half way up.

put agar and dishes in pc for 45 minutes. After that leave them to chill.
When you can hold agar in your hand and not catch 2o burns put agar and plates in front of flow hood. Flow hood don't work well in dusty or filthy room, if you work in that kind of environment use glove box.
If using glove box use alcohol a lot but don't use any other chemicals like aerosol or asepsol. Wipe bottle, Plastic bag, or jar with plates and box.
wipe the gloves really good. Now wait let the alcohol evaporate, leaving the box just a little bit open. now close the box pour the agar in the plates. plates should be one on another so you don't get much condensation, but watch out once plates are full (full to the middle :smile: ) you don't move them too much if agar is spilt or touching the cover don't use that plate. Now let them chill. if using micelium from other agar, jar or mushroom body put Hydrogen H2O2 3% about 8 -12 drops don't spray the hydrogen. If using spores don't use H2O2 at all. Don't use any kind of tape put them in some clean place with light or without.

for isolation use the fastest and the most rizomorphic peaces for transfer, expect rizo after 4-5th transfer with some cultures, and after first transfer with some other.

here is one post so you don't get scared when see mold in your plate :smile: good luck mate.
http://www.shroomery.org/forums/showflat.php/Number/13686884/page/1


--------------------
By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: Hofmann1943]
    #18815951 - 09/08/13 05:05 PM (10 years, 5 months ago)

couldnt i just clean the petri dishes then wrap them in foil to pc?


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflinePussyFart
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Registered: 04/08/12
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18815959 - 09/08/13 05:07 PM (10 years, 5 months ago)

Quote:

Drunk3n Duck said:
couldnt i just clean the petri dishes then wrap them in foil to pc?



If they are glass dishes sure.

Plastic ones will melt.


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: PussyFart]
    #18816100 - 09/08/13 05:46 PM (10 years, 5 months ago)

damn :mad: lol so i have to put them into a jar to resterilize them if i read the comment from hoffman correctly or into a bag like a spawn bag? that would that stop them from melting?


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflinePussyFart
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck] * 1
    #18816115 - 09/08/13 05:51 PM (10 years, 5 months ago)

No...throw them away.

Plastic dishes are sterilized by gamma radiation, and cannot really be resterilized...they are chap and disposable.

By them by the 500 case to save money.


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: PussyFart]
    #18816411 - 09/08/13 07:18 PM (10 years, 5 months ago)

ok then thanks that sux cuz i was more so asking in reguards to the dishes i didnt use but i kept them in the bag and tied off the end of the bag, i figued it was better than nothing :shrug: o well. also does it matter which side of the dish i pour into?


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflinePussyFart
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Registered: 04/08/12
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18816453 - 09/08/13 07:33 PM (10 years, 5 months ago)

Pour into the smaller half.

The smaller half is the bottom, the lid slips over it.


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: PussyFart]
    #18816783 - 09/08/13 09:10 PM (10 years, 5 months ago)

that was wat i had figured but i just wanted to check lol so which would you choose to isolate? B+ AA+ GT PB PE or Orissa


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflinePussyFart
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck] * 1
    #18816791 - 09/08/13 09:12 PM (10 years, 5 months ago)

Quote:

Drunk3n Duck said:
so which would you choose to isolate? B+ AA+ GT PB PE or Orissa



All of them.....

Find good genetics in all of them....


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: PussyFart]
    #18816934 - 09/08/13 09:46 PM (10 years, 5 months ago)

i doubt i have enough petri dishes atm. that is the plan over time, most diffinately. what would be the best way to save the culture, just tape up a petri with the iso and keep refrigerated or go ahead and get actual tubes to store cultures? and from what i understand its common to sector over 5 transfers to get get an iso from ms so that would already be 1/4 of my dishes for just one iso. or should i just wait for fruits from my current grow to choose clones that way i would have to sector less and save some dishes. possibly get 3 isolates from 9 plates or so. what do yall think?


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflinePussyFart
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18816953 - 09/08/13 09:51 PM (10 years, 5 months ago)

Quote:

Drunk3n Duck said:
from what i understand its common to sector over 5 transfers to get get an iso from ms so that would already be 1/4 of my dishes for just one iso.



No, it is common to make 5 transfers before sectoring even occurs, unless cloning from tissue.

You cannot ever know for sure how many transfers it will take to get a non sectoring culture.

Quote:

Drunk3n Duck said:
or should i just wait for fruits from my current grow to choose clones that way i would have to sector less and save some dishes. possibly get 3 isolates from 9 plates or so. what do yall think?



Wait and clone a good looking fruit or 2.

Again, there is no way to know how many transfers it is going to take.

I can almost guarantee you will need more dishes.


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: PussyFart]
    #18818523 - 09/09/13 11:06 AM (10 years, 5 months ago)

Ok thanks that clears things up a lot :smile: and that's what I'll do then hopefully out of about 20 plates I can get 3 isolates from this current grow. I'm just super excited For these AA+ lol


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflineHofmann1943
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Registered: 03/05/09
Posts: 409
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18819516 - 09/09/13 04:22 PM (10 years, 5 months ago)

is that albino A+?
If so oooo man did i have pain in the ass with them. Maybe i catch some bad isolates but even after 3 years of trying i could not get strong mic from it. Very little fruits and after 2-3 flush i goes all bad (mold or bacteria or just stop to pin from no reason)

But definitely worth a try It is one of the best cubes I ever eaten.

When cloning don't clone a clone if you don't want mutants :feelsweirdman:
But they work the same i did not notice any difference, maybe little more subject to mold but i can't swear on that at least for the first few clones of clones but after that genetics just go to hell I think. But the potency is the same.


--------------------
By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.


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Offlinedruranium
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Registered: 09/10/12
Posts: 68
Last seen: 1 year, 6 months
Re: trying agar again advice appreciated :) [Re: Hofmann1943]
    #18820037 - 09/09/13 06:31 PM (10 years, 5 months ago)

This thread is a big help  :thumbup:

Getting ready to start some agar work for the first time...


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OfflineHofmann1943
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Re: trying agar again advice appreciated :) [Re: druranium]
    #18821770 - 09/10/13 02:07 AM (10 years, 5 months ago)

one more thing

it is pure speculation but you should try to sterilize at least one plastic plate to make sure. I do believe Notahacker420 but If i were you I would try one to see it for my self that is just me :smile:

I don't use plastic plates and all i know is PP (polypropilen) will not melt, and PE (Polyetilen) will melt but thickness of the plate is also very important.
just put one in some bowl so you don't mess up your PC.


--------------------
By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: Hofmann1943]
    #18825733 - 09/10/13 10:35 PM (10 years, 5 months ago)

these are from the 8th and are 11 after inoculation from ms. :smile: :dancingbear:

and how did my prep sound for the agar should that work out ok?


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflineMush4Brains
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Registered: 07/31/13
Posts: 4,419
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18825744 - 09/10/13 10:37 PM (10 years, 5 months ago)

Take that foil off.


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OfflinePussyFart
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Re: trying agar again advice appreciated :) [Re: Mush4Brains]
    #18825830 - 09/10/13 11:03 PM (10 years, 5 months ago)

Quote:

Mush4Brains said:
Take that foil off.




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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: PussyFart]
    #18827126 - 09/11/13 10:02 AM (10 years, 5 months ago)

:shocked: not my labels! Lol y should I remove the foil? I don't see how it would inhibit ge it's not tight or anything.:takingnotes:


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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