Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies   Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18815210 - 09/08/13 01:13 PM (10 years, 5 months ago)

Alcohol lamp is a good thing

and glovebox

after glovebox my success went up a lot, before that I got a lot of trich from openair innocs
(got lots of spores in my house)

no contams now in any jars, before it was a bit random...

heat needle redhot between each jar (cross sterilize) , no need to wipe it(can cause contam), but I wipe jars with alcohol pads before innoc (if I don't have alufoil over them) , i.e. LC jars with innoc ports

agar is another good thing, syringes can contam easily / be contamed when you buy it (bacteria i.e.)
bacteria will be noticeable with an unpleasant odor/almost transparent mycellium in the jars instead of bright white
and easy to spot on agar.... although not always easy to isolate away from

after first success I think agar is important (and making sporeprints for future study... place caps on alufoil, put something over them for 24hrs or so - cup or tupperware box i.e.)
cloning without agar I didn't have much luck with, contamed with bacteria
but agar is easy, then you get contam-free syringes (agar->LC i.e.  or agar->grains, or agar->syringe)

when/if you make a glovebox, don't attach gloves to it (still air box)
spray the box with alcohol, wear gloves, heat the needle outside the glovebox till redhot, bring it in
same with inoculation loops, heat redhot outside the glovebox, bring it in
glovebox/stillair box is almost essential IMO, can be hidden under the bed
(but having it on a shelf is even better, less spores there than on the ground)


Edited by lessismore (09/08/13 01:21 PM)


Extras: Filter Print Post Top
OfflineShroomPuncher
The FunGuy
 User Gallery


Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 7 months, 11 days
Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18815215 - 09/08/13 01:14 PM (10 years, 5 months ago)

Here's the jar I'm keeping my eye on.




Bottom



It seems to be colonizing still but there's an orange spot on the mycelium. I think you can see it in the pic.



Here's a smelly jar:





All the smelly jars are developing somewhat like this one. The smell and dark spots remain.


--------------------
Hunting Psillies?
Want a sig or avatar made? Message me. [Prints in exchange would be nice, but not required]

Disclaimer: I in no way engage in violent acts against mushrooms, nor do I support or advocate such behavior. :mushroom2:


Extras: Filter Print Post Top
OfflineShroomPuncher
The FunGuy
 User Gallery


Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 7 months, 11 days
Re: Psilocybe Cubensis B+ (First grow) [Re: lessismore]
    #18815221 - 09/08/13 01:15 PM (10 years, 5 months ago)

Quote:

mio said:
Alcohol lamp is a good thing

and glovebox

after glovebox my success went up a lot, before that I got a lot of trich from openair innocs
(got lots of spores in my house)

no contams now in any jars, before it was a bit random...

heat needle redhot between each jar (cross sterilize) , no need to wipe it(can cause contam), but I wipe jars with alcohol pads before innoc (if I don't have alufoil over them) , i.e. LC jars with innoc ports

agar is another good thing, syringes can contam easily / be contamed when you buy it (bacteria i.e.)
bacteria will be noticeable with an unpleasant odor/almost transparent mycellium in the jars instead of bright white

after first success I think agar is important
cloning without agar I didn't have much luck with, contamed with bacteria
but agar is easy, then you get contam-free syringes (agar->LC i.e.  or agar->grains, or agar->syringe)





Good tips. Thanks! The mycelium in my jar doesn't seem pure white. What do you think?


--------------------
Hunting Psillies?
Want a sig or avatar made? Message me. [Prints in exchange would be nice, but not required]

Disclaimer: I in no way engage in violent acts against mushrooms, nor do I support or advocate such behavior. :mushroom2:


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 14 days
Trusted Cultivator
Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18815237 - 09/08/13 01:20 PM (10 years, 5 months ago)

Sounds to me like you got some major problems there, I would really revisit the teks and try and figure out why.
This is what they should look like.


Except I prefer the jelly jars instead of standard wide mouth. I always have faster colonization times with Jelly Jars.

Also, I like to mix my BRF and Verm dry then add water. I don't know if this is the right way to do it, but I never have contamination issues.


Extras: Filter Print Post Top
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18815253 - 09/08/13 01:24 PM (10 years, 5 months ago)

Most spores start as grey


Be it cubensis spores, trich - and bacteria will look grey too

let it grow out, sniff the jar if it looks 100% colonized, trow it out/PC it if it doesn't look 100% colonized

never sniff jars that aren't 100% colonized

you will be able to tell if there are bacteria/mold in, by sniffing the jars when 100% colonized

moldy/sour/sweet/rotten , unpleasant smell is bad jar
good jars smell like fresh mushrooms, are bright white usually after 100% colonization, and smell so good you want to smell them again

bad jars smell a bit bad, almost hurt the nose, you don't feel like smelling it again

bad jars often smell so bad you can smell it outside the jars by being near them, but not always, bacteria has a faint smell to it sometimes (therefore give cakes a good sniff after 100% colonization, sniff again if in doubt, don't birth cakes you are in doubt about)


Extras: Filter Print Post Top
OfflineShroomPuncher
The FunGuy
 User Gallery


Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 7 months, 11 days
Re: Psilocybe Cubensis B+ (First grow) [Re: lessismore]
    #18815265 - 09/08/13 01:28 PM (10 years, 5 months ago)

I mean I've walked through all my steps, double checked, everything. I was pretty clean and took more steps for sterilization than most of the teks. I'm thinking something happened during inoculation.. maybe I was too slow, candle soot, maybe I 'over-sterilized' equipment and messed with it too much in the process.


I'm just glad I'm able to restart tomorrow. I'll only have enough for about 2 jars but all in all, that should be enough for me.


Quote:

mio said:
Most spores start as grey


Be it cubensis spores, trich - and bacteria will look grey too

let it grow out, sniff the jar if it looks 100% colonized, trow it out/PC it if it doesn't look 100% colonized

never sniff jars that aren't 100% colonized

you will be able to tell if there are bacteria/mold in, by sniffing the jars when 100% colonized

moldy/sour/sweet/rotten , unpleasant smell is bad jar
good jars smell like fresh mushrooms, are bright white usually after 100% colonization, and smell so good you want to smell them again

bad jars smell a bit bad, almost hurt the nose, you don't feel like smelling it again

bad jars often smell so bad you can smell it outside the jars by being near them




Thank you for the advice! Good stuff. :thumbup:


--------------------
Hunting Psillies?
Want a sig or avatar made? Message me. [Prints in exchange would be nice, but not required]

Disclaimer: I in no way engage in violent acts against mushrooms, nor do I support or advocate such behavior. :mushroom2:


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 14 days
Trusted Cultivator
Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18815305 - 09/08/13 01:41 PM (10 years, 5 months ago)

Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.

Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.


Extras: Filter Print Post Top
OfflineShroomPuncher
The FunGuy
 User Gallery


Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 7 months, 11 days
Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
    #18815324 - 09/08/13 01:47 PM (10 years, 5 months ago)

Quote:

Sockadin said:
Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.

Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.





They are, but I was worried about puncturing the aluminum foil. And honestly, I'm extremely broke and a PC isn't an option. I've seen quite a few people grow with good results and without a pressure cooker. No doubt it's more efficient, but it's not even an option right now, sadly.


--------------------
Hunting Psillies?
Want a sig or avatar made? Message me. [Prints in exchange would be nice, but not required]

Disclaimer: I in no way engage in violent acts against mushrooms, nor do I support or advocate such behavior. :mushroom2:


Extras: Filter Print Post Top
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18815360 - 09/08/13 01:59 PM (10 years, 5 months ago)

Quote:

ShroomPuncher said:
Quote:

Sockadin said:
Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.

Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.





They are, but I was worried about puncturing the aluminum foil. And honestly, I'm extremely broke and a PC isn't an option. I've seen quite a few people grow with good results and without a pressure cooker. No doubt it's more efficient, but it's not even an option right now, sadly.




pftek is possible without a PC, but you will get more luck with a PC

$77 for a PC here in most stores, and $60 in many stores

won't be 15 psi at that price likely, but 12 psi is better than a pot

you can also wait, remember to buy the largest PC you can get when you get money, it can't be too large (grain jars are quarts size, and you often need 8 of them)
and good for pasteurizing too, there it needs to be as large as possible as well (straw/coir)
a good 15psi pc is $150+ (presto i.e.) iirc

PC is the most important thing in this hobby

the larger it is the more time you save, PC in the evening, innoc in the morning :wink:
would be nice if I could PC 12 pfjars at once, or 8 grain jars at once, much easier than PCing 2-3 times
and much easier for straw too, have to pasteurize 2-3 times with the small one I got for enough bulk sub

the seal is bad on cheap PCs too often... breaks in no time, then you need to buy a new seal
and if the seal breaks, you just lost all your PCed jars... they will contam, and you won't know the seal broke always


Edited by lessismore (09/08/13 02:05 PM)


Extras: Filter Print Post Top
Offlinetlien
Male

Registered: 10/07/12
Posts: 378
Last seen: 9 years, 4 months
Re: Psilocybe Cubensis B+ (First grow) [Re: lessismore]
    #18815635 - 09/08/13 03:32 PM (10 years, 5 months ago)

I'm in Scandinavia, so there is probably, and I'm not an expert, significantly less microbiology in the environment compared to, say, Florida, due to the relatively low temperature and humidity.

However, I made 12 jars, and only one of them became contaminated.

I steamed them for 90 minutes, I used lots of alcohol on my hands and tools, I used a lighter to sterilize the syringe. I showered and put on clean clothes when I worked.

However, I do not have a pressure cooker, flow hood, glove box, or anything like that.

And I used glasses with double foil layers, no lids.

I even put the glasses in a shopping bag and took them with me on an hour long bus trip the two three days later.

And still, only 1/12 was contaminated.

Since you went WAY beyond my approach, it leads me to suspect your syringe contents were contaminated to begin with.

Did you receive the syringe, or a print? Did you use a fresh and sterile, out-of-the-pack syringe and needle?

But I dunno, if you can get a pressure cooker cheaply, might be worth a shot, this is generally a pretty cheap hobby, one pressure cooker shouldn't break the budget, I know a lot of people are enthusiastic about them, it's just that I had no problems in my own subjective experience leading me to doubt they are crucial.


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 14 days
Trusted Cultivator
Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
    #18815681 - 09/08/13 03:45 PM (10 years, 5 months ago)

Quote:

tlien said:
I'm in Scandinavia, so there is probably, and I'm not an expert, significantly less microbiology in the environment compared to, say, Florida, due to the relatively low temperature and humidity.

However, I made 12 jars, and only one of them became contaminated.

I steamed them for 90 minutes, I used lots of alcohol on my hands and tools, I used a lighter to sterilize the syringe. I showered and put on clean clothes when I worked.

However, I do not have a pressure cooker, flow hood, glove box, or anything like that.

And I used glasses with double foil layers, no lids.

I even put the glasses in a shopping bag and took them with me on an hour long bus trip the two three days later.

And still, only 1/12 was contaminated.

Since you went WAY beyond my approach, it leads me to suspect your syringe contents were contaminated to begin with.

Did you receive the syringe, or a print? Did you use a fresh and sterile, out-of-the-pack syringe and needle?

But I dunno, if you can get a pressure cooker cheaply, might be worth a shot, this is generally a pretty cheap hobby, one pressure cooker shouldn't break the budget, I know a lot of people are enthusiastic about them, it's just that I had no problems in my own subjective experience leading me to doubt they are crucial.





Jars with out lids? Wow that is awesome. Good Looking oUt.

Here is the thing, I see so many people spend
$12-15 per syringe
$5-8  on 12  1/2 pint jars.
$3-4 Bucks on Perlite
$4-5 Bucks on Verm
$2-3 On BRF
Why not drop another $35 on a PC... Best thing I ever bought.

Imagine spending all that money and loosing all 12 jars. Versus investing another 35 and have a really good chance at fruiting so you can make your own isolate or MS print.


Extras: Filter Print Post Top
InvisibleKjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
    #18815691 - 09/08/13 03:47 PM (10 years, 5 months ago)

It was a syringe directly from a sponsor if I'm not mistaken.

Regarding Scandinavian climate; I was thinking the same thing. I mean, I haven't been doing this for long enough to have consistent results, but thus far I haven't even seen contams in the jars where I openly invited for it. During my first inoculation I knocked up the last jar (after doing the rest with proper precautions) in the kitchen in open air, with the used syringe and water directly from the faucet. No flaming, no precautions, sloppy as hell.. performed exactly the same as the rest of my jars.

And yeah; I can't find mason jars with proper wide shoulders, not do I have a PC, so I am using double foil and steam as well.


Extras: Filter Print Post Top
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
    #18815778 - 09/08/13 04:09 PM (10 years, 5 months ago)

Putting drinking glasses with brf in in shopping basket sounds like a bad idea(to the poster mentioning this..)

don't shake pfjars, you disturb the dry verm layer

move them carefully after innoc, and also before innoc (after pc)

I've gotten contams before from tilting my pfjars... trich

prefer to put them on a shelf, they tolerate light fine, it helps during all growth stages


Extras: Filter Print Post Top
OfflineShroomPuncher
The FunGuy
 User Gallery


Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 7 months, 11 days
Re: Psilocybe Cubensis B+ (First grow) [Re: lessismore]
    #18815820 - 09/08/13 04:23 PM (10 years, 5 months ago)

Yup, it was from a sponsor and the needle was out-of-package.


Here's another pic I found on my camera. Don't even remember which jar it is.





And this is the lamp I'll be using for tomorrow.




Better than a candle and now I don't have shaky hands to blame.


And good mention on the tilting. I may have tilted my jars very slightly, but probably not enough to even to disturb the dry verm layer. And I think I went a little thick with my dry verm, too.


--------------------
Hunting Psillies?
Want a sig or avatar made? Message me. [Prints in exchange would be nice, but not required]

Disclaimer: I in no way engage in violent acts against mushrooms, nor do I support or advocate such behavior. :mushroom2:


Extras: Filter Print Post Top
InvisibleKjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18815943 - 09/08/13 05:03 PM (10 years, 5 months ago)

Denatured alcohol is green over there? Haha it makes it look like something out of a bad horror movie. "Ah, my zombie blood is ready!"

It's pinkish red over here... Anyways, the ghettoburner looks to be all good, and I see you have tested it already =)


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 14 days
Trusted Cultivator
Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
    #18815954 - 09/08/13 05:05 PM (10 years, 5 months ago)

IM so lost. Are you innoc another batch? You're problem is in the PC and/or the syringe.


Extras: Filter Print Post Top
InvisibleKjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin] * 1
    #18815964 - 09/08/13 05:09 PM (10 years, 5 months ago)

He is testing the remaining 2-3 ml in the syringe to see if he gets identical results.

I don't think the problem stems from lack of PC as the contamination is happening on the inoculation sites. I think it was introduced by the needle or the spore solution.


Extras: Filter Print Post Top
Offlineheratogwea
 User Gallery


Registered: 05/05/11
Posts: 607
Last seen: 3 years, 9 months
Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
    #18815988 - 09/08/13 05:14 PM (10 years, 5 months ago)

Quote:

Sockadin said:
Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.

Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.




Crap? I have 100% success with steaming so far. Only contams have been due to a bad syringe.


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 14 days
Trusted Cultivator
Re: Psilocybe Cubensis B+ (First grow) [Re: heratogwea]
    #18815999 - 09/08/13 05:20 PM (10 years, 5 months ago)

Quote:

heratogwea said:
Quote:

Sockadin said:
Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.

Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.




Crap? I have 100% success with steaming so far. Only contams have been due to a bad syringe.





Crap! If you are successful, you can print and make your own syringe.

All I'm saying is that if your having trouble with contam's, and your as sterile as this guy maybe it is the method of sterlitity.

We can all wash our hand before we eat. Doesn't mean our hands are clean.


Extras: Filter Print Post Top
Offlineheratogwea
 User Gallery


Registered: 05/05/11
Posts: 607
Last seen: 3 years, 9 months
Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
    #18816007 - 09/08/13 05:22 PM (10 years, 5 months ago)

Quote:

Sockadin said:
Quote:

heratogwea said:
Quote:

Sockadin said:
Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.

Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.




Crap? I have 100% success with steaming so far. Only contams have been due to a bad syringe.





Crap! If you are successful, you can print and make your own syringe.

All I'm saying is that if your having trouble with contam's, and your as sterile as this guy maybe it is the method of sterlitity.

We can all wash our hand before we eat. Doesn't mean our hands are clean.




True. I also don't use a sab, flow hood, or anything like that. I'm just quick and extra clean.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | Next >  [ show all ]

Shop: PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies   Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Psilocybe Cubensis Species (READ for GOOD info)
( 1 2 all )
DannyBoy 58,655 29 11/24/09 02:17 PM
by Roadkill
* Acadian Coast psilocybe cubensis. LearyfanS 8,333 4 04/16/02 05:20 PM
by Roadkill
* Georgia Psilocybe Cubensis Jammer 5,844 19 01/05/10 08:54 PM
by cowfodder
* What Psilocybe Cubensis do you guys enjoy?
( 1 2 all )
Blaxican707 9,355 39 12/27/02 11:58 PM
by Zen Peddler
* psilocybe cubensis...best strain? NewShroomerGuy 30,783 5 08/27/01 11:04 AM
by puscle
* The Foolproof Psilocybe Cubensis tek.. Help noisycricket 3,635 4 06/08/02 06:45 AM
by Workman
* Best tek for Psilocybe cubensis? Most nutrients? PHIPHTIE 2,792 2 04/09/03 09:53 PM
by Raedon
* simple cubensis growing technique PANICNOT 2,657 3 04/05/03 01:41 PM
by PANICNOT

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
12,013 topic views. 26 members, 195 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.008 seconds on 14 queries.