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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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trying agar again advice appreciated :)
#18813709 - 09/08/13 01:08 AM (10 years, 4 months ago) |
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well ive tried agar a couple of times and it didn't work out so well ... but ive decided to try again since i have a bad ass flow hood now . i figured this time i would try pouring into some pre sterilized plates as well as trying again at no pour. for the no pour i mixed 200ml of water with 2g of elme and 2g agar and placed into various small jars then pc'd for 45 min and let cool. prepping to pour the agar i mixed 500ml water with 10g elme and 9g agar into an empty liquor bottle and pc'd for 15 min with lid loose on the bottle but no filter in lid. after about 30-45 min i removed bottle from pc and poured a total of 11 petri dishes with it but i think i filled them a little high... lol i then wrapped them in micro pore tape...that was a bad idea lol next time i will let them cool in front of the flow hood and then wrap them. but other than that is there anything i maybe should do or try differently the next time i make a batch of agar? and which culture should i attempt to isolate for my newly made agar PE, PB, B+ GT, Orissa, or AA+? i'll log the iso for advice too i've started a new grow with PE, PB, and AA+. AA+ deffinately looks to be doing the best of the ms from each.
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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Hofmann1943
explorer



Registered: 03/05/09
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18813984 - 09/08/13 03:27 AM (10 years, 4 months ago) |
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hahahahah yea i know how frustrated agars can be. Here is a couple of tips for you as a friend.
First thing first
Wash all plates well then put them in plastic bag for sterilization of some bigger jar the point is while sterilizing them non of boiling water should break in them only steam.
When making agar use some kind of Jar or bottle that wont leak all over the place when pouring agar in to petri dishes. Yes make the lid loose. 4% remember that it means on 96 ml of distillate water 4g of dry matter
2g malt 2g agar agar
measure your plates how much water is enough to fill it to the middle. Then you will know how much of agar you will need for how much dishes.
Use dishes not bigger than 10 cm in diameter but use deeper dishes 1.5 - 2 cm don't fill them more than a half way up.
put agar and dishes in pc for 45 minutes. After that leave them to chill. When you can hold agar in your hand and not catch 2o burns put agar and plates in front of flow hood. Flow hood don't work well in dusty or filthy room, if you work in that kind of environment use glove box. If using glove box use alcohol a lot but don't use any other chemicals like aerosol or asepsol. Wipe bottle, Plastic bag, or jar with plates and box. wipe the gloves really good. Now wait let the alcohol evaporate, leaving the box just a little bit open. now close the box pour the agar in the plates. plates should be one on another so you don't get much condensation, but watch out once plates are full (full to the middle ) you don't move them too much if agar is spilt or touching the cover don't use that plate. Now let them chill. if using micelium from other agar, jar or mushroom body put Hydrogen H2O2 3% about 8 -12 drops don't spray the hydrogen. If using spores don't use H2O2 at all. Don't use any kind of tape put them in some clean place with light or without.
for isolation use the fastest and the most rizomorphic peaces for transfer, expect rizo after 4-5th transfer with some cultures, and after first transfer with some other.
here is one post so you don't get scared when see mold in your plate good luck mate. http://www.shroomery.org/forums/showflat.php/Number/13686884/page/1
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: Hofmann1943]
#18815951 - 09/08/13 05:05 PM (10 years, 4 months ago) |
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couldnt i just clean the petri dishes then wrap them in foil to pc?
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18815959 - 09/08/13 05:07 PM (10 years, 4 months ago) |
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Quote:
Drunk3n Duck said: couldnt i just clean the petri dishes then wrap them in foil to pc?
If they are glass dishes sure.
Plastic ones will melt.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: PussyFart]
#18816100 - 09/08/13 05:46 PM (10 years, 4 months ago) |
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damn lol so i have to put them into a jar to resterilize them if i read the comment from hoffman correctly or into a bag like a spawn bag? that would that stop them from melting?
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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PussyFart
Retired Cultivation Extrodinaire



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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck] 1
#18816115 - 09/08/13 05:51 PM (10 years, 4 months ago) |
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No...throw them away.
Plastic dishes are sterilized by gamma radiation, and cannot really be resterilized...they are chap and disposable.
By them by the 500 case to save money.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: PussyFart]
#18816411 - 09/08/13 07:18 PM (10 years, 4 months ago) |
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ok then thanks that sux cuz i was more so asking in reguards to the dishes i didnt use but i kept them in the bag and tied off the end of the bag, i figued it was better than nothing o well. also does it matter which side of the dish i pour into?
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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PussyFart
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18816453 - 09/08/13 07:33 PM (10 years, 4 months ago) |
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Pour into the smaller half.
The smaller half is the bottom, the lid slips over it.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: PussyFart]
#18816783 - 09/08/13 09:10 PM (10 years, 4 months ago) |
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that was wat i had figured but i just wanted to check lol so which would you choose to isolate? B+ AA+ GT PB PE or Orissa
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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PussyFart
Retired Cultivation Extrodinaire



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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck] 1
#18816791 - 09/08/13 09:12 PM (10 years, 4 months ago) |
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Quote:
Drunk3n Duck said: so which would you choose to isolate? B+ AA+ GT PB PE or Orissa
All of them.....
Find good genetics in all of them....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: PussyFart]
#18816934 - 09/08/13 09:46 PM (10 years, 4 months ago) |
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i doubt i have enough petri dishes atm. that is the plan over time, most diffinately. what would be the best way to save the culture, just tape up a petri with the iso and keep refrigerated or go ahead and get actual tubes to store cultures? and from what i understand its common to sector over 5 transfers to get get an iso from ms so that would already be 1/4 of my dishes for just one iso. or should i just wait for fruits from my current grow to choose clones that way i would have to sector less and save some dishes. possibly get 3 isolates from 9 plates or so. what do yall think?
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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PussyFart
Retired Cultivation Extrodinaire



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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18816953 - 09/08/13 09:51 PM (10 years, 4 months ago) |
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Quote:
Drunk3n Duck said: from what i understand its common to sector over 5 transfers to get get an iso from ms so that would already be 1/4 of my dishes for just one iso.
No, it is common to make 5 transfers before sectoring even occurs, unless cloning from tissue.
You cannot ever know for sure how many transfers it will take to get a non sectoring culture.
Quote:
Drunk3n Duck said: or should i just wait for fruits from my current grow to choose clones that way i would have to sector less and save some dishes. possibly get 3 isolates from 9 plates or so. what do yall think?
Wait and clone a good looking fruit or 2.
Again, there is no way to know how many transfers it is going to take.
I can almost guarantee you will need more dishes.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: PussyFart]
#18818523 - 09/09/13 11:06 AM (10 years, 4 months ago) |
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Ok thanks that clears things up a lot and that's what I'll do then hopefully out of about 20 plates I can get 3 isolates from this current grow. I'm just super excited For these AA+ lol
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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Hofmann1943
explorer



Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18819516 - 09/09/13 04:22 PM (10 years, 4 months ago) |
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is that albino A+? If so oooo man did i have pain in the ass with them. Maybe i catch some bad isolates but even after 3 years of trying i could not get strong mic from it. Very little fruits and after 2-3 flush i goes all bad (mold or bacteria or just stop to pin from no reason)
But definitely worth a try It is one of the best cubes I ever eaten.
When cloning don't clone a clone if you don't want mutants  But they work the same i did not notice any difference, maybe little more subject to mold but i can't swear on that at least for the first few clones of clones but after that genetics just go to hell I think. But the potency is the same.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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druranium
Farmer


Registered: 09/10/12
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Last seen: 1 year, 5 months
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Re: trying agar again advice appreciated :) [Re: Hofmann1943]
#18820037 - 09/09/13 06:31 PM (10 years, 4 months ago) |
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This thread is a big help
Getting ready to start some agar work for the first time...
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Hofmann1943
explorer



Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
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Re: trying agar again advice appreciated :) [Re: druranium]
#18821770 - 09/10/13 02:07 AM (10 years, 4 months ago) |
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one more thing
it is pure speculation but you should try to sterilize at least one plastic plate to make sure. I do believe Notahacker420 but If i were you I would try one to see it for my self that is just me 
I don't use plastic plates and all i know is PP (polypropilen) will not melt, and PE (Polyetilen) will melt but thickness of the plate is also very important. just put one in some bowl so you don't mess up your PC.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: Hofmann1943]
#18825733 - 09/10/13 10:35 PM (10 years, 4 months ago) |
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these are from the 8th and are 11 after inoculation from ms. 
  and how did my prep sound for the agar should that work out ok?
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18825744 - 09/10/13 10:37 PM (10 years, 4 months ago) |
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Take that foil off.
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PussyFart
Retired Cultivation Extrodinaire



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Re: trying agar again advice appreciated :) [Re: Mush4Brains]
#18825830 - 09/10/13 11:03 PM (10 years, 4 months ago) |
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Quote:
Mush4Brains said: Take that foil off.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: PussyFart]
#18827126 - 09/11/13 10:02 AM (10 years, 4 months ago) |
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not my labels! Lol y should I remove the foil? I don't see how it would inhibit ge it's not tight or anything.
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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Hofmann1943
explorer



Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18828097 - 09/11/13 02:41 PM (10 years, 4 months ago) |
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just remove them believe us you need as much GE as you can get.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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PussyFart
Retired Cultivation Extrodinaire



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Loc: Orbiting Earth
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18828476 - 09/11/13 03:57 PM (10 years, 4 months ago) |
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Quote:
Drunk3n Duck said:
not my labels! Lol y should I remove the foil? I don't see how it would inhibit ge it's not tight or anything.
A better question would be what purpose does the foil serve now that the grains are sterile?
Your lids have filters on them....the foil is pointless.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18828551 - 09/11/13 04:18 PM (10 years, 4 months ago) |
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Quote:
Drunk3n Duck said:
not my labels! Lol y should I remove the foil? I don't see how it would inhibit ge it's not tight or anything.
my labels lol thats what i keep dates, strain, how many times the strain has been g2g'd, ect.. from what i understand ge occurs when the higher temps from colonizing grain causes the built up CO2 to circulate and leave the jar while also allowing for O2 to come in. am i wrong? if not i dont see how a loose piece of foil would inhibit ge.
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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Mush4Brains
LOOL HACKED!!!

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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18828565 - 09/11/13 04:20 PM (10 years, 4 months ago) |
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Keep it on then, you're not going to get better answers than "it isn't necessary" and "the possible downsides outweigh any potential benefits."
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Nice Ol Bud
Apprentice


Registered: 09/11/13
Posts: 162
Loc: Mystical Maze of Mushroom...
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Re: trying agar again advice appreciated :) [Re: Mush4Brains]
#18828911 - 09/11/13 05:22 PM (10 years, 4 months ago) |
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Might just want to follow a tek step by step. Here you go! 
http://www.shroomery.org/9427/Grocery-Store-Agar-Tek
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
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Re: trying agar again advice appreciated :) [Re: Nice Ol Bud]
#18829027 - 09/11/13 05:50 PM (10 years, 4 months ago) |
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thanks nice ol bud but i was more so questioning the pouring prep of petri dishes not individually sterilizing jars of agar as i have done this a couple of times and im trying new things and sorry not trying to argue i just like to know the reasoning behind the action. and i really dont see any possible downsides what does it hurt?
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18829033 - 09/11/13 05:52 PM (10 years, 4 months ago) |
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There are a lot of great videos on YouTube on how to pour petris.
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Nice Ol Bud
Apprentice


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Re: trying agar again advice appreciated :) [Re: Mush4Brains]
#18829041 - 09/11/13 05:53 PM (10 years, 4 months ago) |
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Ya youtube should definitely help!
Here ya go!
How to Pour Agar
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tiltajoel
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Re: trying agar again advice appreciated :) [Re: Nice Ol Bud]
#18829375 - 09/11/13 07:06 PM (10 years, 4 months ago) |
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Think of gas as a fluid, which it is.
The flow will be higher when there are less barriers.
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Drunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
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Re: trying agar again advice appreciated :) [Re: tiltajoel]
#18830329 - 09/11/13 10:14 PM (10 years, 4 months ago) |
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ya i know here is one i found really helpful. and ok ill try it with some of the jars i g2g here in the next few day they arent isolates or anything but i figure if it'll make that big of a difference it'll show between ones with foil vs ones with out.
-------------------- Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
  <-- MY BABY!
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Hofmann1943
explorer



Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
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Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
#18831084 - 09/12/13 03:02 AM (10 years, 4 months ago) |
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Well here is an answer to your question. Next time when inoculating your jars, remove foil from just one of them you will see then why non of us keep the foil on. Labels can be written on the jar with marker like Faber-Castell multimarker the one that have little rubber on the back. This one can easily be removed by dish detergent.
Foil on the leads is like the mask on your face there is no need for them and you only get slower GE.
And yes you are right about GE it is exactly like that. Only problem is that CO2 stays under the foil for longer time, so practically you have area under the foil with air and more % of CO2.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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