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OfflineHofmann1943
explorer
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Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18828097 - 09/11/13 02:41 PM (10 years, 4 months ago)

just remove them believe us :wink:
you need as much GE as you can get.


--------------------
By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Trusted Cultivator
Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18828476 - 09/11/13 03:57 PM (10 years, 4 months ago)

Quote:

Drunk3n Duck said:
:shocked: not my labels! Lol y should I remove the foil? I don't see how it would inhibit ge it's not tight or anything.:takingnotes:



A better question would be what purpose does the foil serve now that the grains are sterile?

Your lids have filters on them....the foil is pointless.


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18828551 - 09/11/13 04:18 PM (10 years, 4 months ago)

Quote:

Drunk3n Duck said:
:shocked: not my labels! Lol y should I remove the foil? I don't see how it would inhibit ge it's not tight or anything.:takingnotes:




my labels lol thats what i keep dates, strain, how many times the strain has been g2g'd, ect.. from what i understand ge occurs when the higher temps from colonizing grain causes the built up CO2 to circulate and leave the jar while also allowing for O2 to come in. am i wrong? if not i dont see how a loose piece of foil would inhibit ge.


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18828565 - 09/11/13 04:20 PM (10 years, 4 months ago)

Keep it on then, you're not going to get better answers than "it isn't necessary" and "the possible downsides outweigh any potential benefits."


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InvisibleNice Ol Bud
Apprentice


Registered: 09/11/13
Posts: 162
Loc: Mystical Maze of Mushroom...
Re: trying agar again advice appreciated :) [Re: Mush4Brains]
    #18828911 - 09/11/13 05:22 PM (10 years, 4 months ago)

Might just want to follow a tek step by step.
Here you go! :laugh:

http://www.shroomery.org/9427/Grocery-Store-Agar-Tek


--------------------


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: Nice Ol Bud]
    #18829027 - 09/11/13 05:50 PM (10 years, 4 months ago)

thanks nice ol bud but i was more so questioning the pouring prep of petri dishes not individually sterilizing jars of agar as i have done this a couple of times and im trying new things :smile: and sorry not trying to argue i just like to know the reasoning behind the action. and i really dont see any possible downsides what does it hurt?


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18829033 - 09/11/13 05:52 PM (10 years, 4 months ago)

There are a lot of great videos on YouTube on how to pour petris.


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InvisibleNice Ol Bud
Apprentice


Registered: 09/11/13
Posts: 162
Loc: Mystical Maze of Mushroom...
Re: trying agar again advice appreciated :) [Re: Mush4Brains]
    #18829041 - 09/11/13 05:53 PM (10 years, 4 months ago)

Ya youtube should definitely help!

Here ya go!

How to Pour Agar


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Offlinetiltajoel
Stranger
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Registered: 09/30/10
Posts: 43
Loc: New York, NY; Seattle, WA Flag
Last seen: 1 year, 2 days
Re: trying agar again advice appreciated :) [Re: Nice Ol Bud]
    #18829375 - 09/11/13 07:06 PM (10 years, 4 months ago)

Think of gas as a fluid, which it is.

The flow will be higher when there are less barriers.


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OfflineDrunk3n Duck
Sir trips-a-lot


Registered: 10/04/11
Posts: 306
Last seen: 1 year, 4 months
Re: trying agar again advice appreciated :) [Re: tiltajoel]
    #18830329 - 09/11/13 10:14 PM (10 years, 4 months ago)

ya i know here is one i found really helpful.:smile: and ok ill try it with some of the jars i g2g here in the next few day they arent isolates or anything but i figure if it'll make that big of a difference it'll show between ones with foil vs ones with out.


--------------------
Everything i post is part of a online role-playing fantasy and is entirely untrue. The pictures i post have all been found online.
<-- MY BABY!


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OfflineHofmann1943
explorer
Male User Gallery


Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
Re: trying agar again advice appreciated :) [Re: Drunk3n Duck]
    #18831084 - 09/12/13 03:02 AM (10 years, 4 months ago)

Well here is an answer to your question.
Next time when inoculating your jars, remove foil from just one of them
you will see then why non of us keep the foil on. Labels can be written on the jar with marker like Faber-Castell multimarker the one that have little rubber on the back. This one can easily be removed by dish detergent.

Foil on the leads is like the mask on your face there is no need for them and you only get slower GE.

And yes you are right about GE it is exactly like that. Only problem is that CO2 stays under the foil for longer time, so practically you have area under the foil with air and more % of CO2.


--------------------
By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.


Extras: Filter Print Post Top
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