Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinememberjockey
Travelling w/out moving
Female User Gallery


Registered: 11/07/11
Posts: 741
Loc: Deep South
Last seen: 2 years, 9 months
Agar question
    #18808076 - 09/06/13 04:29 PM (10 years, 5 months ago)

I took some spores I had stored and scraped them into a couple of dishes, I've transferred them several times due to contamination and I read where you could use baby food jars instead of petri dishes & I'm wondering if the jars I have are okay.  First I used the quilted jelly jars but they're too hard to see into.  I can see where the new one's may be harder to get the pieces out since the jar is deeper.  Also when do I know when they're ready to toss a piece into a jar.  It all looks like undesirable cottony sectors to me.
Thanks.:mushroom2:


--------------------


Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 2 months, 14 days
Re: Agar question [Re: memberjockey]
    #18809931 - 09/06/13 11:12 PM (10 years, 5 months ago)

I don't have experience with agar in jars but another shroomerite said that the additional headspace (airspace) was not a good idea, because it would take too long for the co2 to build up. That's why petri dishes are good, because there is very little air in them.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,811
Loc: Canada Flag
Trusted Cultivator
Re: Agar question [Re: invitro]
    #18810009 - 09/06/13 11:48 PM (10 years, 5 months ago)

If your growing pans (which I'm assuming you are) you may have to do a few transfers before you will see any sectors. Pan mycellium is very tomentose on agar compared to cubes. I found that sectors do show up after a while, but they won't look the way cubes do.

Looks like you got a contam in that one plate in the first pic, I would probably pitch that. The other two look ready to transfer. Can't really see through the jars, you'll only know when you open them.

I am not a huge fan of using 0.5 pint jars for agar. I have done it, but if your looking for something improvised, I like the pp5 mini round container. Easier to see through,easier to manipulate the lid, takes less space in the fridge.



Lids are easier to deal with, but can get loose after a few PC cycles. Pretty much the only drawback. Still I get a pack of 8 for $4 at the grocery store.


Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: Agar question [Re: Pastywhyte]
    #18810134 - 09/07/13 12:52 AM (10 years, 5 months ago)

Why are there a bunch of sudsy bubbles coming out of your agar/mycilium. I don't even... :confused:


Extras: Filter Print Post Top
Offlinememberjockey
Travelling w/out moving
Female User Gallery


Registered: 11/07/11
Posts: 741
Loc: Deep South
Last seen: 2 years, 9 months
Re: Agar question [Re: Pastywhyte]
    #18810786 - 09/07/13 08:31 AM (10 years, 5 months ago)

At first every single plate had contamination I've been transferring the good pieces to new plates and to jars.  I just cut a piece of the mycelium out and transfer it to another container and they seem to be doing okay.  I found some glass petri dishes for about $1.50 a piece I guess I'll have to buy some, I thought it would be easier to sterilize the agar in little reusable jars.  I'll make up some more plates too and compare the growth on both.  Do I use it when it's covered the top of the agar & do I use  just the outer pieces or cut up the whole thing?  Thank you for taking the time to write maybe I could send you a print when I have some.:heart:


--------------------


Edited by memberjockey (09/07/13 08:32 AM)


Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 2 months, 14 days
Re: Agar question [Re: memberjockey]
    #18810812 - 09/07/13 08:47 AM (10 years, 5 months ago)

Quote:

memberjockey said:
  Do I use it when it's covered the top of the agar & do I use  just the outer pieces or cut up the whole thing?  Thank you for taking the time to write maybe I could send you a print when I have some.:heart:




The RR video on petri dishes says to use the center of the petri dish once it's colonized and throw out the outer rim because that is where most of the contam spores will land.  I have found this to be the case in my experience, but I've only done it a few times.  I've tried using the whole dish when no contams are present and cutting that up and putting it into a grain jar.  The contam rate on those jars was very high!  Also RR recommends making the xfer before the whole plate is colonized, i.e. before the outer rim is colonized because the mycelium is more vigorous I think.  Sounds like a good practice since the outer part isn't used anyhow.  I leave the outer 1.5 cm of agar and take the inner part.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,811
Loc: Canada Flag
Trusted Cultivator
Re: Agar question [Re: invitro]
    #18810977 - 09/07/13 09:39 AM (10 years, 5 months ago)

Agreed. That culture that I posted is as far as I let them go before inoculation.


Extras: Filter Print Post Top
Offlinememberjockey
Travelling w/out moving
Female User Gallery


Registered: 11/07/11
Posts: 741
Loc: Deep South
Last seen: 2 years, 9 months
Re: Agar question [Re: Pastywhyte]
    #18811902 - 09/07/13 03:05 PM (10 years, 5 months ago)

Thanks a lot I appreciate the help I've got a stack of plastic sterile plates & I'm sterilizing some agar right now, btw that's not suds in the dish it's condensation.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Agar question sill_o_cin 4,006 4 11/21/01 05:23 PM
by sill_o_cin
* Agar questions-I may have goofed blahblahblah 3,295 6 01/11/02 02:58 PM
by ar393
* Re: Attn: ShroomGod, agar questions vivid 2,693 9 01/12/00 03:49 PM
by Anonymous
* Re: agar questions Anonymous 1,409 6 05/30/00 03:07 PM
by Anonymous
* Agar Questions cantara 5,218 6 08/09/01 06:22 AM
by ar393
* Agar Question TerribleInstict 1,381 4 11/10/01 11:40 PM
by anima
* Agar Questions - Fluffy cloud like Growth indicaz 1,954 9 01/30/03 01:01 PM
by Anonymous
* Spores on agar question .More info plz tripndicular 4,373 5 09/13/02 05:27 PM
by tripndicular

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
531 topic views. 23 members, 165 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 12 queries.