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mathieu
Humble newb



Registered: 08/30/12
Posts: 211
Loc: Texas
Last seen: 2 years, 11 months
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Possible red contam on popcorn GLC jars
#18811000 - 09/07/13 09:49 AM (10 years, 4 months ago) |
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Hello e'rry buddy... I could use some help, either confirmation & consolation or happy news.
I've colonized 4x pint jars of popcorn, each knocked up with 1CC B+ MS 18 days ago. (figured I could break one during extraction, fk up another one's innoc, have one stall, and still have one left over )
I realize that there are better grains (generally rye, or grass seed if you read The Gospel According to Violet), but I wanted to be sure I had more color visibility into contams for my first GLC run. Please take a look at the first image and let me know if I'm kosher to carry on? I've included my entire process up until now-- if you find any issues please give me a heads up?
I know popcorn is prone to some degree of yellow-red tone coloration, so this shade of red left over from undigested cartenoids is perhaps within reason? I shook this jar a couple days sooner than the others, and it's showing just a bit more reddening here and there which makes me nervous. The reddening is exclusive to the kernels themselves, and does not spread onto the myc. the one I'm worried about is #3 / 4 GLC jars; #4 is near 100% and totally white at this point. If there is any similar redness going on, it's covered up in pretty. All jars have a little too much water, with a couple small droplets that form in the bottom against the myc @ night when they're not evaporated to humidity during the light cycle. I'll make sure to dry the popcorn more thoroughly next time-- I let myself be in a rush that day which was stupid.
Jar #3 - in question

Jar #4 - looks to be 

MY ENTIRE PROCESS UP TO NOW
Sterilization
- store bought, extra-buttered popcorn soaked for 24~30 hrs to germinate. (kidding-- it's kettle corn) (kidding, it's caramel corn)
- simmered for 1hr until they could be broken through with a fingernail @ moderate force
- dried for 30 minutes in freshly-bleached towels
- pressed in newspaper to check remaining moisture
- loaded 1/2 full into pint jars (TL-style lids, with EZ felt filters.
- PC'd for 100 minutes @ 15psi
- cooled in the PC & STAYED LEFT THE PC FOR ~48 hrs before getting knocked up (I know this isn't ideal, but it's what happened
)
Inoculation
- room sprayed top to bottom with lysol, fan turned off & door closed for ~18 hours prior to work
/showprofile.php?User=229579]TL style[/url], come to think) with hair covered in a freshly laundered hoodie
- inoculation conducted in a TL-style SAB), which was cleaned sprayed again with lysol and then wiped down with a shop towel soaked in 70% rubbing alcohol. inserted 4x jars thru tyvek arm holes
- showered & wore a bandana over my mouth (also TL style come to think)
- rewiped SAB and jars with alcohol (30 min after 1st)
- I used a fresh, refrigerated 10cc B MS syringe sourced from a sponsor (rhymes with "Whore Perks"); syringe and 18g needle came factory wrapped & sterile (as claimed)
- Flamed the needle red-hot with a lighter, allowed to cool & reflamed between each inoc
- SAB full of alcohol fumes exploded, further sterilizing the syringe, popcorn, and my face (this did not actually happen)
- inoculated jars with ~1CC each, in order 1-4 to track any contam mistakes during inoc / slight differences in amount of solution/visible spores deposited (minimal difference; please note that #3 is the one in question, #4 = pristine white).
Colonization
- jars were left 100% undisturbed (for 8 hours before I got all oogly in the morning
). The spores all ended up @ the bottom of the jars for the most part and colonized from there prior to the shake
- jars given a 12/12 indirect light/dark cycle @ 85F with a standard (non-ideal) incandescent light in the closet.
- spores showed visible germination @ 5 days
- shook jars @ 20% colonization
- jars opened for cat to smell and bat @ 21% colonization (kidding again)
- 1 jar which was shook particularly hard showed blue-green spots w/in 24 hrs-- they never spread and quickly got covered up in rebounding myc: seem to have just been bruised myc (confirmed in RR's notes)
- a couple jars were put on the windowsill for natural light as a test; they seem to have colonized a little faster, but truth be told they were a wee bit ahead anyway.
- small water dropplets now forming @ the bottom of the jar during dark cycle; no discoloration and confirmed liquid
-------------------- Exemplify.
Edited by mathieu (09/07/13 10:04 AM)
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Re: Possible red contam on popcorn GLC jars [Re: mathieu]
#18811066 - 09/07/13 10:14 AM (10 years, 4 months ago) |
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That looks like normal colonized popcorn to me, and popcorn is the ONLY grain I use so I am very familiar with what it is supposed to look like. I see no contam
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Re: Possible red contam on popcorn GLC jars [Re: LiquidGlass]
#18811068 - 09/07/13 10:15 AM (10 years, 4 months ago) |
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The jar should be more full though . . .
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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Jimmyhunter1000
That guy.



Registered: 06/15/12
Posts: 777
Loc: Massachusetts, USA.
Last seen: 5 years, 9 months
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Re: Possible red contam on popcorn GLC jars [Re: LiquidGlass]
#18811081 - 09/07/13 10:21 AM (10 years, 4 months ago) |
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Looks good to me. I'm certain that's just a bit of the color of the kernel showing through the Myc.
-------------------- There isn't a trail too long or too wide.
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mathieu
Humble newb



Registered: 08/30/12
Posts: 211
Loc: Texas
Last seen: 2 years, 11 months
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Re: Possible red contam on popcorn GLC jars [Re: Jimmyhunter1000]
#18811083 - 09/07/13 10:22 AM (10 years, 4 months ago) |
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Quote:
LiquidGlass said: That looks like normal colonized popcorn to me, and popcorn is the ONLY grain I use so I am very familiar with what it is supposed to look like. I see no contam
fantastic-- thanks for the hope, Liquid!
Quote:
Jimmyhunter1000 said: Looks good to me. I'm certain that's just a bit of the color of the kernel showing through the Myc.
I don't remember the corn quite being that red; does the myc leeching out nutrients seem to cause that? I'm also cautious that the other jars aren't quite as red...
Contaminated 0 Healthy 2
I have noticed that partially colonized popcorn kernels seem to lose a little bit of their bright yellow vitality. Moving forward in early grows I think I'll keep a spare jar that hasn't been knocked up to the side; if the "control" jar contams, it's likely that the same thing is going on in the knocked up jars, regardless of myc coverage...
-------------------- Exemplify.
Edited by mathieu (09/07/13 10:24 AM)
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Sockadin



Registered: 01/03/10
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Last seen: 2 months, 21 days
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Re: Possible red contam on popcorn GLC jars [Re: mathieu]
#18814208 - 09/08/13 07:36 AM (10 years, 4 months ago) |
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Its good>>>>>
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mathieu
Humble newb



Registered: 08/30/12
Posts: 211
Loc: Texas
Last seen: 2 years, 11 months
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Re: Possible red contam on popcorn GLC jars [Re: Sockadin]
#18814277 - 09/08/13 08:14 AM (10 years, 4 months ago) |
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sweeet. 3-0... I'm extracting GLC from 3x jars & inoculating today. I'm going for a particularly dry PF block & rehydrating with ~30CC each. should speed things along.
-------------------- Exemplify.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
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Re: Possible red contam on popcorn GLC jars [Re: mathieu]
#18816184 - 09/08/13 06:11 PM (10 years, 4 months ago) |
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That's normal. It's from the mushroom mycelium colonizing it, presumably from the enzymes it produces. I don't think the effect is limited to just popcorn spawn but that's definitely where it's most noticeable.
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mathieu
Humble newb



Registered: 08/30/12
Posts: 211
Loc: Texas
Last seen: 2 years, 11 months
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Re: Possible red contam on popcorn GLC jars [Re: Kizzle]
#18816225 - 09/08/13 06:23 PM (10 years, 4 months ago) |
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Kizz, may I just say that those are some pretty soul-crushing contam pics in your signature link...
a pitch-black hockey puck cake looks viable next to some of those lol.
-------------------- Exemplify.
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