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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
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Re: incubating chamber got to 105 degrees F by accident [Re: SpitballJedi]
    #18804906 - 09/05/13 08:05 PM (10 years, 5 months ago)

It is still debated but I have left uninoculated jars for months at a time. I've never seen, smelled or had any idication of contams. The only thing that happens is they very slowly dry up.

I don't deny that endospores probably survive sterilization. That's not what I question.

I don't believe they actually recover in any amount of time that would be a threat to your jar...if the jar was properly PC'd and kept at good colonizing temps (86F is the max I've tried).

I left one jar out from each batch for a while, I saw no signs of bacteria after months of sitting at room temp or incubated.

Another argument against the endospore-recovery question is that a lot of people (including at least one TC I know of) prep their grains with no-soak preps. I have not heard of them having issues with bacteria because they did not germinate the endospores before PCing.

So...sure, endospores are there and dormant (damaged from the PC cycle) in your jar, I have no evidence that this is not true.

I just don't believe they will recover in time to pose a threat. Everything I've seen points to this being true.

My two cents on the topic :thumbup:


--------------------

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You should take a look. :hehehe:


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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18804929 - 09/05/13 08:12 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
It is still debated but I have left uninoculated jars for months at a time. I've never seen, smelled or had any idication of contams. The only thing that happens is they very slowly dry up.




That was exactly my experience.

Your arguments are very reasonable and I believe them to be true. Thx for indulging me.


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Edited by SpitballJedi (09/05/13 08:37 PM)


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Offlinenepter627
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Registered: 06/20/13
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Re: incubating chamber got to 105 degrees F by accident [Re: SpitballJedi]
    #18805234 - 09/05/13 09:21 PM (10 years, 5 months ago)

built my tit today after reading how fast myc colonizes in it.

turned out the submersible heater i bought wasnt working nor heating my water.

(cheap walmart heater)
so tomorrow im going to return it and buy one with an adjustable tempature gauge.


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: nepter627]
    #18805937 - 09/06/13 12:34 AM (10 years, 5 months ago)

Quote:

nepter627 said:
built my tit today after reading how fast myc colonizes in it.

turned out the submersible heater i bought wasnt working nor heating my water.

(cheap walmart heater)
so tomorrow im going to return it and buy one with an adjustable tempature gauge.




Make sure to get an air pump and an air stone/bubble wand.

It is very important to circulate that water, otherwise you will have hot spots in your incubator.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinespacechildo
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Registered: 01/24/13
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18806452 - 09/06/13 07:53 AM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
Quote:

nepter627 said:
built my tit today after reading how fast myc colonizes in it.

turned out the submersible heater i bought wasnt working nor heating my water.

(cheap walmart heater)
so tomorrow im going to return it and buy one with an adjustable tempature gauge.




Make sure to get an air pump and an air stone/bubble wand.

It is very important to circulate that water, otherwise you will have hot spots in your incubator.




This is probably why my tit failed! The heater kept turning off even though the water had turned cold.
It always failed after about a week.


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InvisibleMykes logos
homo nosce te ipsum
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Registered: 08/05/12
Posts: 1,108
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18806533 - 09/06/13 08:33 AM (10 years, 5 months ago)

i've always looked at it like it's a "race" between contams and myc.... so although leaving jars sitting un-inoculated isn't a big deal, I always try to nocc em up within a day or two. the contams are always there no matter what... you'd have to sterilize for like what, like 12 hours to completely kill off everything? so I try to go from plate to fruiting as fast as possible no matter what the situation is.. but I've rarely lost a jar over the years no matter what I was doing

I know a mycologist out west who soaks his rye 20-24 hours, then skips the boil altogether, and only sterilizes for 60 mins. After a few years of experimentation with many mediums, that's what he stuck with... but he also used wild rice and stuff like that a lot. there's a million ways to do it and they all work just the same. It's just like gardening or anything like this, ask 100 gardeners how to start seeds and you'll get 100 different answers..

What I really wanted to say tho is that I live in south FL and can set colonizing jars anywhere I want and anytime of the year without worrying about the temperature haha... ahhh living in the subtropics :sunny:


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OfflineCaptainPuffy
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Re: incubating chamber got to 105 degrees F by accident [Re: Mykes logos]
    #18806562 - 09/06/13 08:43 AM (10 years, 5 months ago)

I built one of Frank's TiTs to spec. The mycellium seem to grow like clockwork since I've switched to using one. Shake at one week from MS. 100% a week later.

I love my TiT!


Edited by CaptainPuffy (09/06/13 08:44 AM)


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InvisibleMykes logos
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Re: incubating chamber got to 105 degrees F by accident [Re: CaptainPuffy]
    #18806580 - 09/06/13 08:48 AM (10 years, 5 months ago)

Quote:

CaptainPuffy said:
I love my TiT!




haha... kudos to Frank for naming it that


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Offlinenepter627
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18808399 - 09/06/13 05:35 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
Quote:

nepter627 said:
built my tit today after reading how fast myc colonizes in it.

turned out the submersible heater i bought wasnt working nor heating my water.

(cheap walmart heater)
so tomorrow im going to return it and buy one with an adjustable tempature gauge.




Make sure to get an air pump and an air stone/bubble wand.

It is very important to circulate that water, otherwise you will have hot spots in your incubator.





so should i not turn it on until i have an airpump and and air stone?


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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: incubating chamber got to 105 degrees F by accident [Re: Mykes logos]
    #18808506 - 09/06/13 05:57 PM (10 years, 5 months ago)

Quote:

Mykes logos said:
Quote:

CaptainPuffy said:
I love my TiT!




haha... kudos to Frank for naming it that




TiT was around before Frank was a member


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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Offlinenepter627
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Re: incubating chamber got to 105 degrees F by accident [Re: SpitballJedi]
    #18809996 - 09/06/13 11:42 PM (10 years, 5 months ago)

so i got a pump and a bubble stone.

now my question is my water in the tub suppose to erupt every 30=90 seconds?

water doesnt seem to be splashing over it was at first but i took some water out.

now it just erupts  thats the only word i can think of to describe whats happening.


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: nepter627]
    #18810871 - 09/07/13 09:11 AM (10 years, 5 months ago)

Yep, I call it "the bloop" and it is how your water will be circulating.

No need to fill it so full it splashes. I keep my water about halfway up the side of my inner tote :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinenepter627
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Registered: 06/20/13
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18811413 - 09/07/13 12:25 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
Yep, I call it "the bloop" and it is how your water will be circulating.

No need to fill it so full it splashes. I keep my water about halfway up the side of my inner tote :thumbup:





okay great that means im doing something right, =]

and i releveled the water after the first splash and it has yet to splash so i think im good.


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Offlineghostinthemist
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Registered: 08/01/13
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Re: incubating chamber got to 105 degrees F by accident [Re: nepter627]
    #18855344 - 09/17/13 08:23 PM (10 years, 5 months ago)

IM BACK IN THE SADDLE AGAIN!


RUN AND HIDE!


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InvisibleDreaming Nomad
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Registered: 01/15/13
Posts: 908
Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18855422 - 09/17/13 08:45 PM (10 years, 5 months ago)

:somefunnyshit:

Hahahahahahahaha oh man, that is rich!


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They say curiosity killed the cat...
Fortunately, I am not a cat.


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