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memberjockey
Travelling w/out moving



Registered: 11/07/11
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Agar question
#18808076 - 09/06/13 04:29 PM (10 years, 5 months ago) |
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I took some spores I had stored and scraped them into a couple of dishes, I've transferred them several times due to contamination and I read where you could use baby food jars instead of petri dishes & I'm wondering if the jars I have are okay. First I used the quilted jelly jars but they're too hard to see into. I can see where the new one's may be harder to get the pieces out since the jar is deeper. Also when do I know when they're ready to toss a piece into a jar. It all looks like undesirable cottony sectors to me.  Thanks.
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invitro


Registered: 05/03/13
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Last seen: 2 months, 14 days
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I don't have experience with agar in jars but another shroomerite said that the additional headspace (airspace) was not a good idea, because it would take too long for the co2 to build up. That's why petri dishes are good, because there is very little air in them.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Agar question [Re: invitro]
#18810009 - 09/06/13 11:48 PM (10 years, 5 months ago) |
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If your growing pans (which I'm assuming you are) you may have to do a few transfers before you will see any sectors. Pan mycellium is very tomentose on agar compared to cubes. I found that sectors do show up after a while, but they won't look the way cubes do.
Looks like you got a contam in that one plate in the first pic, I would probably pitch that. The other two look ready to transfer. Can't really see through the jars, you'll only know when you open them.
I am not a huge fan of using 0.5 pint jars for agar. I have done it, but if your looking for something improvised, I like the pp5 mini round container. Easier to see through,easier to manipulate the lid, takes less space in the fridge.

Lids are easier to deal with, but can get loose after a few PC cycles. Pretty much the only drawback. Still I get a pack of 8 for $4 at the grocery store.
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krypto2000
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Registered: 12/05/06
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Why are there a bunch of sudsy bubbles coming out of your agar/mycilium. I don't even...
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memberjockey
Travelling w/out moving



Registered: 11/07/11
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At first every single plate had contamination I've been transferring the good pieces to new plates and to jars. I just cut a piece of the mycelium out and transfer it to another container and they seem to be doing okay. I found some glass petri dishes for about $1.50 a piece I guess I'll have to buy some, I thought it would be easier to sterilize the agar in little reusable jars. I'll make up some more plates too and compare the growth on both. Do I use it when it's covered the top of the agar & do I use just the outer pieces or cut up the whole thing? Thank you for taking the time to write maybe I could send you a print when I have some.
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Edited by memberjockey (09/07/13 08:32 AM)
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 2 months, 14 days
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Quote:
memberjockey said: Do I use it when it's covered the top of the agar & do I use just the outer pieces or cut up the whole thing? Thank you for taking the time to write maybe I could send you a print when I have some.
The RR video on petri dishes says to use the center of the petri dish once it's colonized and throw out the outer rim because that is where most of the contam spores will land. I have found this to be the case in my experience, but I've only done it a few times. I've tried using the whole dish when no contams are present and cutting that up and putting it into a grain jar. The contam rate on those jars was very high! Also RR recommends making the xfer before the whole plate is colonized, i.e. before the outer rim is colonized because the mycelium is more vigorous I think. Sounds like a good practice since the outer part isn't used anyhow. I leave the outer 1.5 cm of agar and take the inner part.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Agar question [Re: invitro]
#18810977 - 09/07/13 09:39 AM (10 years, 5 months ago) |
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Agreed. That culture that I posted is as far as I let them go before inoculation.
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memberjockey
Travelling w/out moving



Registered: 11/07/11
Posts: 741
Loc: Deep South
Last seen: 2 years, 9 months
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Thanks a lot I appreciate the help I've got a stack of plastic sterile plates & I'm sterilizing some agar right now, btw that's not suds in the dish it's condensation.
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