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OfflineMAEjin
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Weird spawn mass in syringe
    #18807893 - 09/06/13 03:47 PM (10 years, 4 months ago)

I have made my own syringes and used em successfully once but now that I wanna use em again, I am noticing there are what looks like frog spawn in there... that black stuff (spores??) is stuck in slimy translucent frog spawn like texture.
Should I use that stuff? What's happening.

The way I made those syringes is I picked a shroom cap, and dropped it into a 250ml mason jar. After it printed, I injected 100ml boiled cooled water in the jar and shook it. Siphoned it back into the syringe and that's it. I have had around 75% success with the first batch. I used 250ml tall jars with brf cakes pf tek.

Any idea what I might have done wrong?


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OfflineMAEjin
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Re: Weird spawn mass in syringe [Re: MAEjin]
    #18811984 - 09/07/13 03:29 PM (10 years, 4 months ago)

bumpâge!


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Offlinesmegzilla69
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Re: Weird spawn mass in syringe [Re: MAEjin]
    #18811992 - 09/07/13 03:35 PM (10 years, 4 months ago)

Pic would help but sounds like ur spores germinated


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OfflineKizzle
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Re: Weird spawn mass in syringe [Re: smegzilla69]
    #18812052 - 09/07/13 03:58 PM (10 years, 4 months ago)

:whathesaid:
Did you make them with tap water instead of distilled water?


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InvisibleHypnotoadCroaked
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Re: Weird spawn mass in syringe [Re: MAEjin]
    #18812054 - 09/07/13 03:59 PM (10 years, 4 months ago)

Quote:

MAEjin said:
I have made my own syringes and used em successfully once but now that I wanna use em again, I am noticing there are what looks like frog spawn in there... that black stuff (spores??) is stuck in slimy translucent frog spawn like texture.
Should I use that stuff? What's happening.




I had the EXACT same issue with a few self made syringes in the last month.  I used bottled filtered water and a drop of jet dry to reduce clumping.  I can safely say that spores have germinated within my syringes.  I tested them immediately after making the syringe and can confirm that they are clean on agar and grain, but since then they have definitely germinated.  Its funny looking because prior to germination, there was nothing visible.  Now there are black clumps that you described exactly.  I thought the water I used would be good enough to avoid this, but filtered and distilled are 2 different things.


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OfflineMAEjin
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Re: Weird spawn mass in syringe [Re: MAEjin]
    #18812612 - 09/07/13 07:01 PM (10 years, 4 months ago)

I used reverse osmosis water (1-2ppm) ... which is extremely close to distilled water!!!
So does that mean that all these syringes are bad and shouldn't/can't be used?
damniiiiT!! ;p
so using distilled water would fix that problem in the future?


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OfflineStromriderM
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Re: Weird spawn mass in syringe [Re: MAEjin]
    #18812631 - 09/07/13 07:06 PM (10 years, 4 months ago)

You can most certainly use them. You will see growth very quickly. It is like a lc


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InvisibleHypnotoadCroaked
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Re: Weird spawn mass in syringe [Re: Stromrider]
    #18812658 - 09/07/13 07:13 PM (10 years, 4 months ago)

Quote:

psillyshroomer said:
You can most certainly use them. You will see growth very quickly. It is like a lc



In my situation, I can believe that the spores have/will consume the few nutes in the water relatively quick and then "stall/hold".  If we do not ensure that we get live culture into our jars, they may show no growth.  The other thing may be that not all the spores germinated.  The clumping irritates me, because I can see it.....whereas before there was nothing visible to the naked eye.  I have jars ready for a PC run tomorrow morning, and I was going to g2g.....now I am interested in seeing how this setup goes.


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OfflineStromriderM
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Re: Weird spawn mass in syringe [Re: HypnotoadCroaked]
    #18812685 - 09/07/13 07:22 PM (10 years, 4 months ago)

Well this happened to me once and I shook the shit out of the syringe before I used it and I saw growth in 3 days


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OfflineMAEjin
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Re: Weird spawn mass in syringe [Re: Stromrider]
    #18829689 - 09/11/13 08:07 PM (10 years, 4 months ago)

I'll try it out then cause I have like 8 more syringes like that...
I'll post back the results here.
Thanks for the input once again guys!


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Offlinejonnymushroom
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Re: Weird spawn mass in syringe [Re: MAEjin]
    #18829707 - 09/11/13 08:10 PM (10 years, 4 months ago)

Ive had this many times and it worked out great.:smile:


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OfflineMAEjin
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Re: Weird spawn mass in syringe [Re: jonnymushroom]
    #18907514 - 09/29/13 03:10 PM (10 years, 3 months ago)

Ok so here are the results for those interested...
Out of 24 cakes, 3 went bad, but in a odd way!! 1 trichoderma, then 1 black and 1 red.. any ideas if I could still use those two cakes?

4 of the cakes colonized good enough,
9 of them partially colonized, i'll need to re inoculate.. is this doable? 2 weeks later?
8 cakes didn't even colonized at all...
I am trying with RO water this time, cause I am not sure if that is what I used last time but if it doesn't work, I am gonna move on to distilled water and see if it helps!



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Offlinespacechildo
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Re: Weird spawn mass in syringe [Re: MAEjin]
    #18907574 - 09/29/13 03:22 PM (10 years, 3 months ago)

Those pic's are priceless!
But, no, I wouldn't birthed anyone of them.

Also, don't re-inoculate. They probably stalled for a reason, make new ones :wink:

That pinkish in the middle must be the perfect example of lipstick mold!
Ask thmetyl if maybe he wants the pics for his Contams-thread :thumbup:


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OfflineKizzle
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Re: Weird spawn mass in syringe [Re: spacechildo]
    #18908219 - 09/29/13 06:15 PM (10 years, 3 months ago)

The water wouldn't be responsible for the contaminants.

I don't know why I didn't mention it before but your syringe making technique has some serious flaws. A mushroom cap it covered in contaminants especially on the top which mean the jar will also have relatively large amount of contaminants. So by adding the water to the jars like that it's soaking up all of the contaminants everywhere in the jar. Instead you should always transfer the spores to the sterile water and not vice versa.

Also to achieve the lowest possible contamination rate for your syringes you should use the limit the amount of spores used in each syringe. Using more spores than necessary means a higher risk of picking up contaminated spores than necessary.

One other thing, to avoid cross contamination each syringe should be made separately. By making the spore solution for all of them at once in the same water you're going to end up with multiple contaminated syringes where you would otherwise only have a single contaminated syringe.


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OfflineSockadin
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Re: Weird spawn mass in syringe [Re: Kizzle]
    #18908343 - 09/29/13 06:39 PM (10 years, 3 months ago)

:badshroom:


  So have you considered the Shot Glass Tek?

Printing is a really easy process, and can be done with ease.

Also did you clean your syringes before you made the print to syringe transfer? The reason we are all asking is that the molds are right in the inoculation sites. I get a pot of boiling water on the stove. I mean Boiling!!! Then I suck up the water into the syringe 3-4 times and squirt it out into the sink, not back in the pot. Then I Flame sterilize the needle, and alcohol swab a bottle of distilled water and poke my needle into the side of the bottle, suck up 10cc's and recap. This sterilized clean water can then be used to make my syringe from a good clean spore print inside my SAB.

  I wish you the best bud.


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