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Invisiblebulkgrownoob
Registered: 03/21/11
Posts: 345
Isolating a strain from grain jar *PICS*
    #18807493 - 09/06/13 01:34 PM (10 years, 5 months ago)



It's a bit harder to see in the first pic, but in the 2nd pic you can clearly see some individual rhizo strands poking out a good distance above the grains. If I was to take a scalpel and very carefully slice off 1 single strand from that area and transfer it to agar, would I automatically have an isolate off the bat? And would the 1 strand have a hard time surviving alone in the Petri dish or would it be just fine?


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OfflinePussyFart
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Re: Isolating a strain from grain jar *PICS* [Re: bulkgrownoob]
    #18807501 - 09/06/13 01:36 PM (10 years, 5 months ago)

Quote:

bulkgrownoob said:
It's a bit harder to see in the first pic, but in the 2nd pic you can clearly see some individual rhizo strands poking out a good distance above the grains. If I was to take a scalpel and very carefully slice off 1 single strand from that area and transfer it to agar, would I automatically have an isolate off the bat?



Not likely....

Quote:

bulkgrownoob said:
And would the 1 strand have a hard time surviving alone in the Petri dish or would it be just fine?



Chances are it would grow out and sector...


Edited by PussyFart (09/06/13 01:36 PM)


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Invisiblebulkgrownoob
Registered: 03/21/11
Posts: 345
Re: Isolating a strain from grain jar *PICS* [Re: PussyFart]
    #18807687 - 09/06/13 02:34 PM (10 years, 5 months ago)

Thanks for the quick reply. I assumed that 1 individual strand from the jar would be just like taking a wedge from a sector on agar but I guess not. I'm sure it would at least get me real close to an isolate though but I really don't know too much about agar so I could be wrong


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OfflinePussyFart
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Re: Isolating a strain from grain jar *PICS* [Re: bulkgrownoob]
    #18807737 - 09/06/13 02:51 PM (10 years, 5 months ago)

It would get you close, but there would most likely still be sectors....


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