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mthawkins
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What's up with the slow colonizing? Pics
#18806660 - 09/06/13 09:18 AM (10 years, 4 months ago) |
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I've had my WBS jars colonizing for about 3/4 weeks now. 2 out of the 7 jars have not shown any sign of myc except a lighter tint of seeds on the inoculation side. The other 5 are showing growth except 3 of those 5 are going VERY slow, as you can see in the pictures. What should I do to speed it up? I currently have them in a closed cabinet above my refrigerator.
Should I shake any?

-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
Edited by mthawkins (09/06/13 09:19 AM)
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klak1212
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18806683 - 09/06/13 09:25 AM (10 years, 4 months ago) |
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The jars that have that brown/yellow/gold sludge liquid against the glass are bacterial contaminated, empty them and clean them well.
The ones that have lots of mycellium and no sludge, shake them up by banging the jars on a bike tire, a shoe or phonebook to break up the mycellium and spread it throughout the jars.
If you see any signs of the liquid sludge stuff against the glass in any jars, you need to start over, you can't use them to spawn and the mycellium will not colonize those parts. The bacteria will quickly take over the jars and make it a mess.
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PhosCap
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18806685 - 09/06/13 09:26 AM (10 years, 4 months ago) |
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Put a thermometer in your cabinet so you know exactly what the temps are. Faster colonizing temps are around 78-83. I always like to keep mine around 78-80. As per the slow jars. The one on the right has bacterial problems, toss it. The one on the left possibly has problems aswell.
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mthawkins
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Re: What's up with the slow colonizing? Pics [Re: PhosCap]
#18806715 - 09/06/13 09:35 AM (10 years, 4 months ago) |
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Quote:
klak1212 said: The jars that have that brown/yellow/gold sludge liquid against the glass are bacterial contaminated, empty them and clean them well.
The ones that have lots of mycellium and no sludge, shake them up by banging the jars on a bike tire, a shoe or phonebook to break up the mycellium and spread it throughout the jars.
If you see any signs of the liquid sludge stuff against the glass in any jars, you need to start over, you can't use them to spawn and the mycellium will not colonize those parts. The bacteria will quickly take over the jars and make it a mess.
Wow. So only 2 of my 7 jars were successful? Would you say the only good ones are the ones at the far left? How would I be able to spawn bulk just 2 jars 
I just banged up my two big jars. The myc was hard as a brick and didn't break up, but I still gave it a good banging and rolling the jars. Nearly all of the jars have that discolor or liquid shit, even the one with the second most growth.
-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
Edited by mthawkins (09/06/13 09:41 AM)
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klak1212
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18806810 - 09/06/13 10:07 AM (10 years, 4 months ago) |
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Quote:
mthawkins said:
Quote:
klak1212 said: The jars that have that brown/yellow/gold sludge liquid against the glass are bacterial contaminated, empty them and clean them well.
The ones that have lots of mycellium and no sludge, shake them up by banging the jars on a bike tire, a shoe or phonebook to break up the mycellium and spread it throughout the jars.
If you see any signs of the liquid sludge stuff against the glass in any jars, you need to start over, you can't use them to spawn and the mycellium will not colonize those parts. The bacteria will quickly take over the jars and make it a mess.
Wow. So only 2 of my 7 jars were successful? Would you say the only good ones are the ones at the far left? How would I be able to spawn bulk just 2 jars 
I just banged up my two big jars. The myc was hard as a brick and didn't break up, but I still gave it a good banging and rolling the jars. Nearly all of the jars have that discolor or liquid shit, even the one with the second most growth.
The first 2 pictures with lots of healthy looking mycellium look great. Breaking up the mycellium and spreading it throughout will speed up colonization of those jars ten-fold. Keep an eye on them though, because chances are that if the other jars have that bacteria contam, the 2 healthy jars may just as well.
I would start over with the other jars though, but in my opinion, the mycelium will cease to grow and the sludge will spread further and get uglier.
Basic: Make a smaller tub with the 2 jars.
Advanced: Make a Still Air Box. Throw the contam jars out, clean them thoroughly and start new grain jars (working on better moisture content and better sterile procedure through boiling the grains and pressure cooking them for the recommended times, making sure the filters in the lids dont get wet). Grain to Grain transfer the 2 healthy jars in the still air box to the new jars you just made(as long as one of them has no contam present when they're at 100% colonization, you can use it to transfer to up to 10 new grain jars).
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mthawkins
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Re: What's up with the slow colonizing? Pics [Re: klak1212]
#18806968 - 09/06/13 11:00 AM (10 years, 4 months ago) |
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Klak, I feel like all of the jars, even the strong ones, have some discoloring. Pictures later. I did shake them up, but couldn't break up the myc. Just a big ball that I moved around lol. What would be the cause of this? I brought my seeds to a boil, pc at 15psi for 90 minutes. Should I clean out the inside of jars before adding seeds or something?
I don't have any more spores of this particular kind, so ill try g2g. My Lids have rtv and polyfill. Any step by step procedure to g2g?
-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
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dDrey
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18807058 - 09/06/13 11:29 AM (10 years, 4 months ago) |
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Don't trip, i noccd up mine on 8-10 and there only 90%.... SUPER SLOW... and more then half contamd.
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mthawkins
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Re: What's up with the slow colonizing? Pics [Re: dDrey]
#18807239 - 09/06/13 12:12 PM (10 years, 4 months ago) |
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That really sucks man. im going to try g2g and hope for the best. What's the proper way to shake the jars? Should I shake till the massive myc ball is broken up? All I did was move seeds around it, couldn't break it up.
-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
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klak1212
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18807316 - 09/06/13 12:32 PM (10 years, 4 months ago) |
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Hit it up against anything you can to break up the ball of mycelium, without breaking the glass jar. Anything rubber works great, bike tire, sole of a shoe/workboot, palm of your hand, or phonebook. You want it broken up good so all the mycelium grains are broken up, and then spread throughout the jar so they can colonize their surrounding grains. Then hope it recovers, and if it does you can use it for G2G as long as you see no contamination.
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mthawkins
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Re: What's up with the slow colonizing? Pics [Re: klak1212]
#18807332 - 09/06/13 12:37 PM (10 years, 4 months ago) |
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Thanks for the tip! Ill look up how to properly do g2g. How can I avoid this type of contam next tjme
-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
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klak1212
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18807349 - 09/06/13 12:42 PM (10 years, 4 months ago) |
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Quote:
mthawkins said: Thanks for the tip! Ill look up how to properly do g2g. How can I avoid this type of contam next tjme
I've never worked with WBS, but with my rye jars if the grains are too wet, that's a breeding ground for bacteria. Id like to say your jars are too wet, so maybe cut back on the soak time and also lengthen the amount of time you let the water steam/dry off before loading the jars.
As long as your pressure cooking (90 minutes, 15psi, build up pressure first for 10 mins then put rocker on)and sterile inoculation procedures are looked after (flame needle to red hot, wipe down innoc port with IPA, dont wipe needle), that should fix your problem.
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mthawkins
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Re: What's up with the slow colonizing? Pics [Re: klak1212]
#18841196 - 09/14/13 02:00 PM (10 years, 4 months ago) |
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I shook up my jars last week, and the main one that was colonizing well prior is now taking a long time. I'm rather disappointed with my first batch since its taken a month dealing with this now. I injected more jars a week or two ago and they're barely showing. 2/7 show a small amount near the bottom of the jars.
I have another syringe I plan on working with shortly.. Except I'm focusing on g2g and will inject 2cc into 5 jars instead of 7. Also, I will keep it warmer in my apartment.
-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
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mthawkins
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18848116 - 09/16/13 08:42 AM (10 years, 4 months ago) |
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Should I keep my apartment warm? I usually have the temp at 70 at night.. I have no fans. Could that be why? If so, ill just buy some fans lol
-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
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Stromrider
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18848121 - 09/16/13 08:45 AM (10 years, 4 months ago) |
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Anywhere between 70 and 80 degrees is fine
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mthawkins
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Re: What's up with the slow colonizing? Pics [Re: Stromrider]
#18848136 - 09/16/13 08:52 AM (10 years, 4 months ago) |
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Then it must be colder in my cabinet. I really need a thermometer. Like what I said in my previous post, I just don't understand why it's taking so incredibly long.. And since I shook its going even slower.
-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
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Stromrider
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18848158 - 09/16/13 09:00 AM (10 years, 4 months ago) |
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After you shake it takes the grains 24 to 48 hours just to recover and start growing again. You pretty confident that the moisture content is correct in your jars?
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Stromrider
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Re: What's up with the slow colonizing? Pics [Re: Stromrider]
#18848161 - 09/16/13 09:01 AM (10 years, 4 months ago) |
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They look a little wet in the first pics. That can slow or sometimes stall growth
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mthawkins
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Re: What's up with the slow colonizing? Pics [Re: Stromrider]
#18848181 - 09/16/13 09:07 AM (10 years, 4 months ago) |
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A lot of my jars got bacteria from, what I assume, me not drying it enough. I shook the jars over a week ago and it's barely doing anything. I inoculated 7 more jars over a week ago, and they're barely showing as well. A few have some growing on the bottom at least.
I have one more syringe that I'm going to work with, 2cc per jar. Dry seeds real well, then do g2g.
-------------------- “Never laugh at live dragons.” - J.R.R Tolkien
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Stromrider
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Re: What's up with the slow colonizing? Pics [Re: mthawkins]
#18848232 - 09/16/13 09:28 AM (10 years, 4 months ago) |
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Doesn't sound too good bud. Usually if you shake a 20 to 30% colonized jar they are done in a few days
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PrinceShroom
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Re: What's up with the slow colonizing? Pics [Re: Stromrider]
#18848238 - 09/16/13 09:31 AM (10 years, 4 months ago) |
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2 cc's per jar is not good. .75ml per is usually the best. If you over load the jar with spores you have competing genetics which can slow your jars down. I doubt thats the case for your slow jars I say they are a little wet is your issue
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  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
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