Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDeadPhan
Male User Gallery

Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
I think I may have found my contam source.
    #18803556 - 09/05/13 02:28 PM (10 years, 4 months ago)

Not wearing a facial mask in front of flowhood.  :facepalm:  I had a successful run at grain to grains once because I either spaced or didn't have a mask.  I think I recall being very careful though and holding my breath and when breathing turning my head to the side. After I took a break and started some innoculations again I think I felt like my flowhood was so bad assbecause of my short run of 100%success I considered certain measures as overkill.
I was having some bad runs.  So good runs but usually always had at least one contam in a batch of g2g's. I kept thinking spawn or from somewhere on the lid. Well, all I know now is I have clean agar and left some dishes out the other night with lid off and am waiting to see if they produce contam. 
I'm not certain yet but I also did a 2 batches of ms jar innocs as well. The first batch without a mask and I lost about half to contam.  The most recent batch, although a week or so behind, has no signs of contam as of yet and that was with a mask.  I'm knocking on wood here, but, feeling foolish, as well as relieved that my naïveté might have been the sole cause. 
I kept thinking somehow my filter got dirty and that was really bumming me out.  Anyway, hopefully I'm right about this and I can get back on track to yummy sterility!  :justcantwait:


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
Trusted Cultivator
Re: I think I may have found my contam source. [Re: DeadPhan]
    #18803559 - 09/05/13 02:30 PM (10 years, 4 months ago)

I don't see how your breath would make it past the flow of sterile air....

If that is the case, maybe you should work closer to the front of the filter...

:shrug:  Just sayin...


Edited by PussyFart (09/05/13 02:30 PM)


Extras: Filter Print Post Top
OfflineDeadPhan
Male User Gallery

Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: I think I may have found my contam source. [Re: PussyFart]
    #18803577 - 09/05/13 02:35 PM (10 years, 4 months ago)

My flow is a little on the slower side of things.  I posted a video a while back of my flow test. It's more of a lil past 45 degrees. I was told that is not so much a bad thing. But I'm thinking maybe on the subject of breath or any movement in the room possibly?  Ill try to find my video.


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
OfflineBlake_Shroom
Stranger
 User Gallery


Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 2 months
Re: I think I may have found my contam source. [Re: PussyFart]
    #18803591 - 09/05/13 02:38 PM (10 years, 4 months ago)

If I don't wear tyvek sleeves I get a lot bacterial contams. If your breath is an issue, wear a mask. Also, make sure your nose is behind the jar in respect to the flow hood when sniffing the jars to do a grain to grain


Extras: Filter Print Post Top
OfflineDeadPhan
Male User Gallery

Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: I think I may have found my contam source. [Re: DeadPhan]
    #18803598 - 09/05/13 02:39 PM (10 years, 4 months ago)

My original post concerning my laminar flow. I have since gotten a legit 3m prefilter immediately following this post and it didn't change the flow at all.  http://www.shroomery.org/forums/showflat.php/Number/16980874#16980874


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
OfflineDeadPhan
Male User Gallery

Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: I think I may have found my contam source. [Re: Blake_Shroom]
    #18803601 - 09/05/13 02:40 PM (10 years, 4 months ago)

Quote:

Blake_Shroom said:
If I don't wear tyvek sleeves I get a lot bacterial contams. If your breath is an issue, wear a mask. Also, make sure your nose is behind the jar in respect to the flow hood when sniffing the jars to do a grain to grain




Hmm. I've never wore tyvek sleeves.


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
OfflineDeadPhan
Male User Gallery

Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: I think I may have found my contam source. [Re: DeadPhan]
    #18803639 - 09/05/13 02:50 PM (10 years, 4 months ago)

Also I always work right against the filter. Maybe I'm wrong. If there is a contam in an MS syringe would the whole syringe not produce contams?  It's odd because its a reliable vendor I got from, and I got contams from every single of the five varietys I inoculated with. But from all of them some came out clean. The latest run of innoc's are clean.  Well soon I should be able to grow out this agar into mono's and just make masters from that and see what happens. And then back to g2g's. this really has me kinda stumped As I had a good run back around a year ago.  Granted it was around October and into December.  I'm wondering if the cold weather helped with contams in the air.  But if that was the case, then my flowhood isn't up to par anyway as it should work just fine in any climate and environment, no?


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Flowhood prefilter Wheedhead! 1,092 6 08/11/03 10:45 PM
by hyper_dermic
* Prefilter for HEPA ? MrMaddHatter 1,155 5 06/08/05 03:41 PM
by MrMaddHatter
* Safe to uses this as a prefilter? NTTG_Hunter 756 4 10/07/03 10:58 AM
by NTTG_Hunter
* prefilter for hepa? cant figure out blower, have read faq. kilgore_trout 1,116 4 08/26/04 07:26 PM
by kilgore_trout
* Big-time contam problem.Pleeeez help
( 1 2 all )
Numba9 2,804 22 08/17/01 05:59 AM
by ShadowTH
* Adding prefilter to flowhood ozzysmygod 795 9 05/26/09 08:06 PM
by ozzysmygod
* grow box/light exposure and source DexTek 1,053 5 08/21/02 09:54 AM
by apatheticgoddess
* whats the best way to get contams out of carpet? victimlesstribe 1,781 13 01/29/04 10:57 AM
by MycoFactory

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
237 topic views. 44 members, 250 guests and 21 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.008 seconds on 14 queries.