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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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I think I may have found my contam source.
#18803556 - 09/05/13 02:28 PM (10 years, 4 months ago) |
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Not wearing a facial mask in front of flowhood. I had a successful run at grain to grains once because I either spaced or didn't have a mask. I think I recall being very careful though and holding my breath and when breathing turning my head to the side. After I took a break and started some innoculations again I think I felt like my flowhood was so bad assbecause of my short run of 100%success I considered certain measures as overkill. I was having some bad runs. So good runs but usually always had at least one contam in a batch of g2g's. I kept thinking spawn or from somewhere on the lid. Well, all I know now is I have clean agar and left some dishes out the other night with lid off and am waiting to see if they produce contam. I'm not certain yet but I also did a 2 batches of ms jar innocs as well. The first batch without a mask and I lost about half to contam. The most recent batch, although a week or so behind, has no signs of contam as of yet and that was with a mask. I'm knocking on wood here, but, feeling foolish, as well as relieved that my naïveté might have been the sole cause. I kept thinking somehow my filter got dirty and that was really bumming me out. Anyway, hopefully I'm right about this and I can get back on track to yummy sterility!
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: I think I may have found my contam source. [Re: DeadPhan]
#18803559 - 09/05/13 02:30 PM (10 years, 4 months ago) |
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I don't see how your breath would make it past the flow of sterile air....
If that is the case, maybe you should work closer to the front of the filter...
Just sayin...
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (09/05/13 02:30 PM)
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: I think I may have found my contam source. [Re: PussyFart]
#18803577 - 09/05/13 02:35 PM (10 years, 4 months ago) |
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My flow is a little on the slower side of things. I posted a video a while back of my flow test. It's more of a lil past 45 degrees. I was told that is not so much a bad thing. But I'm thinking maybe on the subject of breath or any movement in the room possibly? Ill try to find my video.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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Blake_Shroom
Stranger



Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 2 months
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Re: I think I may have found my contam source. [Re: PussyFart]
#18803591 - 09/05/13 02:38 PM (10 years, 4 months ago) |
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If I don't wear tyvek sleeves I get a lot bacterial contams. If your breath is an issue, wear a mask. Also, make sure your nose is behind the jar in respect to the flow hood when sniffing the jars to do a grain to grain
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: I think I may have found my contam source. [Re: DeadPhan]
#18803598 - 09/05/13 02:39 PM (10 years, 4 months ago) |
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My original post concerning my laminar flow. I have since gotten a legit 3m prefilter immediately following this post and it didn't change the flow at all. http://www.shroomery.org/forums/showflat.php/Number/16980874#16980874
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: I think I may have found my contam source. [Re: Blake_Shroom]
#18803601 - 09/05/13 02:40 PM (10 years, 4 months ago) |
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Quote:
Blake_Shroom said: If I don't wear tyvek sleeves I get a lot bacterial contams. If your breath is an issue, wear a mask. Also, make sure your nose is behind the jar in respect to the flow hood when sniffing the jars to do a grain to grain
Hmm. I've never wore tyvek sleeves.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: I think I may have found my contam source. [Re: DeadPhan]
#18803639 - 09/05/13 02:50 PM (10 years, 4 months ago) |
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Also I always work right against the filter. Maybe I'm wrong. If there is a contam in an MS syringe would the whole syringe not produce contams? It's odd because its a reliable vendor I got from, and I got contams from every single of the five varietys I inoculated with. But from all of them some came out clean. The latest run of innoc's are clean. Well soon I should be able to grow out this agar into mono's and just make masters from that and see what happens. And then back to g2g's. this really has me kinda stumped As I had a good run back around a year ago. Granted it was around October and into December. I'm wondering if the cold weather helped with contams in the air. But if that was the case, then my flowhood isn't up to par anyway as it should work just fine in any climate and environment, no?
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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