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Invisiblejolo
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Registered: 09/26/09
Posts: 166
Isolating and long term storage
    #18803163 - 09/05/13 12:25 PM (10 years, 4 months ago)

If I have a few isolates ready to test out, how do I do this for long term storage?

I'm inoculating master jars, then G2Ging them.

Do I go ahead and stick a little of the isolate in a master slant tube before I even know if I want to preserve it or not? Or, once the mushrooms grow out, if I like it, do I clone one of those mushrooms to agar then put into a master slant?

Or just make sure nothing bad gets on the agar plate during the transfer to the master jar, keep the plate and if it like it transfer a bit from the plate to a slant once I've seen how it performs?


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: Isolating and long term storage [Re: jolo]
    #18803200 - 09/05/13 12:36 PM (10 years, 4 months ago)

Quote:

jolo said:
If I have a few isolates ready to test out, how do I do this for long term storage?

I'm inoculating master jars, then G2Ging them.

Do I go ahead and stick a little of the isolate in a master slant tube before I even know if I want to preserve it or not?



Basically....or wait until you have a good performer and then go back to the original dish and make a slant then.

Either way....it's really up to you...


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Isolating and long term storage [Re: jolo]
    #18803207 - 09/05/13 12:38 PM (10 years, 4 months ago)

Quote:

jolo said:
Or just make sure nothing bad gets on the agar plate during the transfer to the master jar, keep the plate and if it like it transfer a bit from the plate to a slant once I've seen how it performs?




Transfer a small piece to your slant.

Use the rest of the plate to inoculate your jars. You can do this back to back, without ever leaving your SAB (if it's big enough).

If the isolate sucks, just PC the slant and empty the agar.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisiblejolo
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Registered: 09/26/09
Posts: 166
Re: Isolating and long term storage [Re: FrankHorrigan]
    #18803268 - 09/05/13 12:57 PM (10 years, 4 months ago)

Thanks guys!


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